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Volumn 29, Issue 5, 1999, Pages 1532-1542

Immunogene therapy of murine fibrosarcoma using IL-15 gene with high translation efficiency

Author keywords

Alternative cDNA; Gene therapy; IL 15; Meth A tumor

Indexed keywords

COMPLEMENTARY DNA; INTERLEUKIN 15; MESSENGER RNA; OKT 4; OKT 8;

EID: 0032950172     PISSN: 00142980     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-4141(199905)29:05<1532::AID-IMMU1532>3.0.CO;2-Z     Document Type: Article
Times cited : (26)

References (56)
  • 1
    • 0030026798 scopus 로고    scopus 로고
    • Prevention abnormalities in signal; transduction of T cells in cancer: The promise of cytokine gene therapy
    • Zier, K., Gansbacher, B. and Salvadori, S., Prevention abnormalities in signal; transduction of T cells in cancer: the promise of cytokine gene therapy. Immunol Today 1996. 17: 39-45.
    • (1996) Immunol Today , vol.17 , pp. 39-45
    • Zier, K.1    Gansbacher, B.2    Salvadori, S.3
  • 3
    • 0028153727 scopus 로고
    • Augmention of antitumor immunity by tumor cells transduced with a retroviral vector carrying the interleukin-2 and interferon-gamma cDNA
    • Rosenthal, F. M., Cronin, K., Bannerji, R., Golde, D. W. and Gansbacher, B., Augmention of antitumor immunity by tumor cells transduced with a retroviral vector carrying the interleukin-2 and interferon-gamma cDNA. Blood 1994. 83: 1289-1298.
    • (1994) Blood , vol.83 , pp. 1289-1298
    • Rosenthal, F.M.1    Cronin, K.2    Bannerji, R.3    Golde, D.W.4    Gansbacher, B.5
  • 5
    • 0029877716 scopus 로고    scopus 로고
    • Antitumor effects of granulocyte-macrophage colony-stimulating factor production by melanoma cells
    • Armstrong, C. A., Botella, R., Galloway, T. H., Murray, N., Kramp, J. M., Song, I.S. and Ansel, J. C., Antitumor effects of granulocyte-macrophage colony-stimulating factor production by melanoma cells. Cancer Res. 1996. 56: 2191-2198.
    • (1996) Cancer Res. , vol.56 , pp. 2191-2198
    • Armstrong, C.A.1    Botella, R.2    Galloway, T.H.3    Murray, N.4    Kramp, J.M.5    Song, I.S.6    Ansel, J.C.7
  • 6
    • 0027262224 scopus 로고
    • Cytokine secretion by genetically modified nonimmunogenic murine fibrosarcoma. Tumor inhibition by IL-2 but not tumor necrosis factor
    • Karp, S. E., Farber, A., Salo, J. C., Hwu, P., Jaffe, G., Asher, A. L., Shiloni, E., Restifo, N. P., Mule, J. J. and Rosenberg, S. A., Cytokine secretion by genetically modified nonimmunogenic murine fibrosarcoma. Tumor inhibition by IL-2 but not tumor necrosis factor. J. Immunol. 1993. 150: 896-908.
    • (1993) J. Immunol. , vol.150 , pp. 896-908
    • Karp, S.E.1    Farber, A.2    Salo, J.C.3    Hwu, P.4    Jaffe, G.5    Asher, A.L.6    Shiloni, E.7    Restifo, N.P.8    Mule, J.J.9    Rosenberg, S.A.10
  • 7
    • 0031171209 scopus 로고    scopus 로고
    • Immunostimulatory cytokines in somatic cells and gene therapy of cancer
    • Mackensen, A., Lindemann, A. and Mertelsmann, R. J., Immunostimulatory cytokines in somatic cells and gene therapy of cancer. Cytokine Growth Factor Rev. 1997. 8: 119-128.
    • (1997) Cytokine Growth Factor Rev. , vol.8 , pp. 119-128
    • Mackensen, A.1    Lindemann, A.2    Mertelsmann, R.J.3
  • 8
    • 0030940081 scopus 로고    scopus 로고
    • The anti-human tumor effect and generation of human cytotoxic T cells in SCID mice given human peripheral blood lymphocytes by the in vivo transfer of the Interleukin-6 gene using adenovirus vector
    • Tanaka, F., Abe, M., Akiyoshi, T., Nomura, T., Sugimachi, K., Kishimoto, T., Suzuki, T. and Okada, M., The anti-human tumor effect and generation of human cytotoxic T cells in SCID mice given human peripheral blood lymphocytes by the in vivo transfer of the Interleukin-6 gene using adenovirus vector. Cancer Res. 1997. 57: 1335-1343.
    • (1997) Cancer Res. , vol.57 , pp. 1335-1343
    • Tanaka, F.1    Abe, M.2    Akiyoshi, T.3    Nomura, T.4    Sugimachi, K.5    Kishimoto, T.6    Suzuki, T.7    Okada, M.8
  • 9
    • 0026710683 scopus 로고
    • Genetic modification of a murine fibrosarcoma to produce interleukin 7 stimulates host cell infiltration and tumor immunity
    • McBride, W. H., Thacker, J. D., Comora, S., Economou, J. S., Kelley, D., Hogge, D., Dubinett, S. M. and Dougherty, G. J., Genetic modification of a murine fibrosarcoma to produce interleukin 7 stimulates host cell infiltration and tumor immunity. Cancer Res. 1992. 52: 3931-3937.
    • (1992) Cancer Res. , vol.52 , pp. 3931-3937
    • McBride, W.H.1    Thacker, J.D.2    Comora, S.3    Economou, J.S.4    Kelley, D.5    Hogge, D.6    Dubinett, S.M.7    Dougherty, G.J.8
  • 11
    • 0028270141 scopus 로고
    • The role of IL-2 secreted from genetically modified tumor cells in the establishment of antitumor immunity
    • Bannerji, R., Arroyo, C. D., Cordon-Cardo, C. and Gilboa, E., The role of IL-2 secreted from genetically modified tumor cells in the establishment of antitumor immunity. J. Immunol. 1994. 152: 2324-2332.
    • (1994) J. Immunol. , vol.152 , pp. 2324-2332
    • Bannerji, R.1    Arroyo, C.D.2    Cordon-Cardo, C.3    Gilboa, E.4
  • 15
    • 0028363377 scopus 로고
    • The interleukin (IL) 2 receptor β chain is shared by IL-2 and a cytokine, provisionally designated IL-T, that stimulates T-cell proliferation and the induction of lymphokine-activated killer cells
    • Bamford, R. N., Grant, A. J., Burton, J. D., Peters, C., Kury, G., Goldman, C. K., Brennan, J., Roessler, J. and Waldmann, T. A., The interleukin (IL) 2 receptor β chain is shared by IL-2 and a cytokine, provisionally designated IL-T, that stimulates T-cell proliferation and the induction of lymphokine-activated killer cells. Proc. Natl. Acad. Sci. USA 1994. 91: 4940-4944.
    • (1994) Proc. Natl. Acad. Sci. USA , vol.91 , pp. 4940-4944
    • Bamford, R.N.1    Grant, A.J.2    Burton, J.D.3    Peters, C.4    Kury, G.5    Goldman, C.K.6    Brennan, J.7    Roessler, J.8    Waldmann, T.A.9
  • 17
    • 0028818320 scopus 로고
    • Endogenous production of interleukin 15 by activated human monocytes is critical for optimal production of interferon-gamma by natural killer cells in vitro
    • Carson, W. E., Ross, M. E., Baiocchi, R. A., Marien, M. J., Boiani, N., Grabstein, K. and Caligiuri, M. A., Endogenous production of interleukin 15 by activated human monocytes is critical for optimal production of interferon-gamma by natural killer cells in vitro. J. Clin. Invest. 1995. 96: 2578-2582.
    • (1995) J. Clin. Invest. , vol.96 , pp. 2578-2582
    • Carson, W.E.1    Ross, M.E.2    Baiocchi, R.A.3    Marien, M.J.4    Boiani, N.5    Grabstein, K.6    Caligiuri, M.A.7
  • 18
    • 0028798403 scopus 로고
    • IL-15 has stimulatory activity for the induction of cell proliferation and differentiation
    • Armitage, R. J., Macduff, B. M., Eisenman, J., Paxton, R. and Grabstein, K. H., IL-15 has stimulatory activity for the induction of cell proliferation and differentiation. J. Immunol. 1995. 154: 483-490.
    • (1995) J. Immunol. , vol.154 , pp. 483-490
    • Armitage, R.J.1    Macduff, B.M.2    Eisenman, J.3    Paxton, R.4    Grabstein, K.H.5
  • 20
    • 0030714613 scopus 로고    scopus 로고
    • Interleukin-15 preferentially promotes the growth of intestinal intraepitherial lymphocytes bearing γδ T cell receptor in mice
    • Inagaki-Ohara, K., Nishimura, H., Mitani, A. and Yoshikai, Y., Interleukin-15 preferentially promotes the growth of intestinal intraepitherial lymphocytes bearing γδ T cell receptor in mice. Eur. J. Immunol. 1997. 27: 2885-2891.
    • (1997) Eur. J. Immunol. , vol.27 , pp. 2885-2891
    • Inagaki-Ohara, K.1    Nishimura, H.2    Mitani, A.3    Yoshikai, Y.4
  • 23
    • 0028825961 scopus 로고
    • Interleukin 15 induction of lymphokine-activated killer cell function against autologous tumor cells in melanoma patient lymphocytes by a CD18-dependent, perforin-related mechanism
    • Gamero, A. M., Ussery, D., Reintgen, D. S., Puleo, C. A. and Djeu, J. Y., Interleukin 15 induction of lymphokine-activated killer cell function against autologous tumor cells in melanoma patient lymphocytes by a CD18-dependent, perforin-related mechanism. Cancer Res. 1995. 55: 4988-4994.
    • (1995) Cancer Res. , vol.55 , pp. 4988-4994
    • Gamero, A.M.1    Ussery, D.2    Reintgen, D.S.3    Puleo, C.A.4    Djeu, J.Y.5
  • 24
    • 0030453771 scopus 로고    scopus 로고
    • Induction by interleukin-15 of human killer cell activity against lung cancer cell lines and its regulatory mechanisms, Jan. J.
    • Takeuchi, E., Yanagawa, H., Yano, S., Haku, T. and Sone, S., Induction by interleukin-15 of human killer cell activity against lung cancer cell lines and its regulatory mechanisms, Jan. J. Cancer Res. 1996. 87: 1251-1258.
    • (1996) Cancer Res. , vol.87 , pp. 1251-1258
    • Takeuchi, E.1    Yanagawa, H.2    Yano, S.3    Haku, T.4    Sone, S.5
  • 25
    • 0031563157 scopus 로고    scopus 로고
    • IL-15 mediates anti-tumor effects after cyclophosphamide injection of tumorbearing mice and enhances adoptive immunotherapy: The potential role of NK cell subpopulations
    • Evans, R., Fuller, J. A., Christianson, G., Krupke, D. M. and Troutt, A. B., IL-15 mediates anti-tumor effects after cyclophosphamide injection of tumorbearing mice and enhances adoptive immunotherapy: the potential role of NK cell subpopulations. Cell. Immunol. 1997. 179: 66-73.
    • (1997) Cell. Immunol. , vol.179 , pp. 66-73
    • Evans, R.1    Fuller, J.A.2    Christianson, G.3    Krupke, D.M.4    Troutt, A.B.5
  • 27
    • 0029946063 scopus 로고    scopus 로고
    • IL-15: A pleiotropic cytokine with diverse receptor/signaling pathways whose expression is controlled at multiple levels
    • Tagaya, Y., Bamford, R. N., DeFilippis, A. P. and Waldmann, T. A., IL-15: a pleiotropic cytokine with diverse receptor/signaling pathways whose expression is controlled at multiple levels. Immunity 1996. 4: 329-336.
    • (1996) Immunity , vol.4 , pp. 329-336
    • Tagaya, Y.1    Bamford, R.N.2    DeFilippis, A.P.3    Waldmann, T.A.4
  • 28
    • 0030639208 scopus 로고    scopus 로고
    • Regulation of IL-15 secretion via the leader peptide of two IL-15 isoforms
    • Onu, A., Pohl, T., Krause, H. and Bulfone-Paus, S., Regulation of IL-15 secretion via the leader peptide of two IL-15 isoforms. J. Immunol. 1997. 158: 255-262.
    • (1997) J. Immunol. , vol.158 , pp. 255-262
    • Onu, A.1    Pohl, T.2    Krause, H.3    Bulfone-Paus, S.4
  • 29
    • 0030008255 scopus 로고    scopus 로고
    • Interleukin (IL) 15/IL-T production by the adult T-cell leukemia cell line Hut-102 is associated with a human T-cell lymphotrophic virus type I R region/IL-15 fusion message that lacks many upstream AUGs that normally attenuate IL-15 mRNA translation
    • Bamford, R. N., Battiata, A. P., Burton, J. D., Sharma, H. and Waldmann, T. A., Interleukin (IL) 15/IL-T production by the adult T-cell leukemia cell line Hut-102 is associated with a human T-cell lymphotrophic virus type I R region/IL-15 fusion message that lacks many upstream AUGs that normally attenuate IL-15 mRNA translation. Proc. Natl. Acad. Sci. USA 1996. 93: 2897-2902.
    • (1996) Proc. Natl. Acad. Sci. USA , vol.93 , pp. 2897-2902
    • Bamford, R.N.1    Battiata, A.P.2    Burton, J.D.3    Sharma, H.4    Waldmann, T.A.5
  • 30
    • 0029933669 scopus 로고    scopus 로고
    • IL-15: The role of translational regulation in their expression
    • Bamford, R. N., Battiata, A. P. and Waldmann, T. A., IL-15: The role of translational regulation in their expression. J. Leukoc. Biol. 1996. 54: 476-480.
    • (1996) J. Leukoc. Biol. , vol.54 , pp. 476-480
    • Bamford, R.N.1    Battiata, A.P.2    Waldmann, T.A.3
  • 31
    • 0030938636 scopus 로고    scopus 로고
    • Expression of two interleukin-15 mRNA isoforms in human tumors does not correlate with secretion: Role of different signal peptides
    • Mezza, R., Gaggero, A., Neglia, F., Basso, S., Soforzini, S., Pereno, R., Azzarone, B. and Ferrini, S., Expression of two interleukin-15 mRNA isoforms in human tumors does not correlate with secretion: role of different signal peptides. Eur. J. Immunol. 1997. 27: 1049-1054.
    • (1997) Eur. J. Immunol. , vol.27 , pp. 1049-1054
    • Mezza, R.1    Gaggero, A.2    Neglia, F.3    Basso, S.4    Soforzini, S.5    Pereno, R.6    Azzarone, B.7    Ferrini, S.8
  • 32
    • 0032080666 scopus 로고    scopus 로고
    • The 5′ untranslated region, signal peptide, and the coding sequence of the carboxyl terminus of IL-15 participate in its multifaceted translational control
    • Bamford, R. N., DeFilippis, A. P., Azimi, N., Kurys, G. and Waldmann, T. A., The 5′ untranslated region, signal peptide, and the coding sequence of the carboxyl terminus of IL-15 participate in its multifaceted translational control. J. Immunol. 1998. 160: 4418-4426.
    • (1998) J. Immunol. , vol.160 , pp. 4418-4426
    • Bamford, R.N.1    DeFilippis, A.P.2    Azimi, N.3    Kurys, G.4    Waldmann, T.A.5
  • 34
    • 0030047144 scopus 로고    scopus 로고
    • Identification of a novel interleukin-15 (IL-15) transcript isoform generated by alternative splicing in human small cell lung cancer cell lines
    • Meazza, R., Verdiani, S., Biassoni, R., Coppolecchia, M., Gaggero, A., Orengo, A. M., Colombo, M. P., Azzarone, B. and Ferrini, S., Identification of a novel interleukin-15 (IL-15) transcript isoform generated by alternative splicing in human small cell lung cancer cell lines. Oncogene 1996. 12: 2187-2192.
    • (1996) Oncogene , vol.12 , pp. 2187-2192
    • Meazza, R.1    Verdiani, S.2    Biassoni, R.3    Coppolecchia, M.4    Gaggero, A.5    Orengo, A.M.6    Colombo, M.P.7    Azzarone, B.8    Ferrini, S.9
  • 35
    • 0031974726 scopus 로고    scopus 로고
    • Translational efficiency is up-regulated by alternative exon in murine IL-15 mRNA
    • Nishimura, H., Washizu, J., Nakamura, N., Enomoto, A. and Yoshikai, Y., Translational efficiency is up-regulated by alternative exon in murine IL-15 mRNA. J. Immunol. 1998. 160: 936-942.
    • (1998) J. Immunol. , vol.160 , pp. 936-942
    • Nishimura, H.1    Washizu, J.2    Nakamura, N.3    Enomoto, A.4    Yoshikai, Y.5
  • 37
    • 0031051547 scopus 로고    scopus 로고
    • Abnormal development of intestinal intraepithelial lymphocytes and peripheral natural killer cells in mice lacking the IL-2 receptor beta chain
    • Suzuki, H., Duncan, G. S., Takimoto, H. and Mak, T. W., Abnormal development of intestinal intraepithelial lymphocytes and peripheral natural killer cells in mice lacking the IL-2 receptor beta chain. J. Exp. Med. 1997. 185: 499-505.
    • (1997) J. Exp. Med. , vol.185 , pp. 499-505
    • Suzuki, H.1    Duncan, G.S.2    Takimoto, H.3    Mak, T.W.4
  • 38
    • 0027237666 scopus 로고
    • Selective long-term elimination of natural killer cells in vivo by an anti-interleukin 2 receptor beta chain monoclonal antibody in mice
    • Tanaka, T., Kitamura, F., Nagasaka, Y., Kuida, K., Suwa, H. and Miyasaka, M., Selective long-term elimination of natural killer cells in vivo by an anti-interleukin 2 receptor beta chain monoclonal antibody in mice. J. Exp. Med. 1993. 178: 1103-1107.
    • (1993) J. Exp. Med. , vol.178 , pp. 1103-1107
    • Tanaka, T.1    Kitamura, F.2    Nagasaka, Y.3    Kuida, K.4    Suwa, H.5    Miyasaka, M.6
  • 40
    • 0030981706 scopus 로고    scopus 로고
    • The host-tumor immune conflict: From immunosuppression to resistance and destruction
    • Chouaib, S., Asselin-Paturel, C., Mami-Chouaib, F., Caignard, A. and Blay, J. Y., The host-tumor immune conflict: from immunosuppression to resistance and destruction. Immunol. Today 1997. 18: 493-497.
    • (1997) Immunol. Today , vol.18 , pp. 493-497
    • Chouaib, S.1    Asselin-Paturel, C.2    Mami-Chouaib, F.3    Caignard, A.4    Blay, J.Y.5
  • 41
    • 0027056895 scopus 로고
    • Alterations in signal transduction molecules in T lymphocytes from tumorbearing mice
    • Mizoguchi, H., O'Shea, J. J., Longo, D. L., Loeffler, C. M., McVicar, D. W., Ochoa, A. C., Alterations in signal transduction molecules in T lymphocytes from tumorbearing mice. Science 1992. 258: 1795-1798.
    • (1992) Science , vol.258 , pp. 1795-1798
    • Mizoguchi, H.1    O'Shea, J.J.2    Longo, D.L.3    Loeffler, C.M.4    McVicar, D.W.5    Ochoa, A.C.6
  • 44
    • 0030584925 scopus 로고    scopus 로고
    • Molecular basis of T cell dysfunction in cancer is influenced by the paracrine secretion of tumor-derived IL-2
    • Salvadori, S. and Zier, K., Molecular basis of T cell dysfunction in cancer is influenced by the paracrine secretion of tumor-derived IL-2. J. Immunol. 1996. 156: 2927-2932.
    • (1996) J. Immunol. , vol.156 , pp. 2927-2932
    • Salvadori, S.1    Zier, K.2
  • 45
    • 0028045991 scopus 로고
    • Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors
    • Salvadori, S., Gansbacher, B., Pizzimenti, A. M. and Zier, K. S., Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors. J. Immunol. 1994. 153: 5176-5182.
    • (1994) J. Immunol. , vol.153 , pp. 5176-5182
    • Salvadori, S.1    Gansbacher, B.2    Pizzimenti, A.M.3    Zier, K.S.4
  • 46
    • 0028965830 scopus 로고
    • Activated macrophages induce structural abnormalities of the T cell receptor-CD3 complex
    • Aoe, T., Okamoto, Y. and Saito, T., Activated macrophages induce structural abnormalities of the T cell receptor-CD3 complex. J. Exp. Med. 1995. 181: 1881-1886.
    • (1995) J. Exp. Med. , vol.181 , pp. 1881-1886
    • Aoe, T.1    Okamoto, Y.2    Saito, T.3
  • 47
    • 0028979704 scopus 로고
    • Rapid turnover of the CD3 zeta chain independent of the TCR-CD3 complex in normal T cells
    • Ono, S., Ohno, H., Saito, T., Rapid turnover of the CD3 zeta chain independent of the TCR-CD3 complex in normal T cells. Immunity 1995. 2: 639-644.
    • (1995) Immunity , vol.2 , pp. 639-644
    • Ono, S.1    Ohno, H.2    Saito, T.3
  • 48
    • 0028244396 scopus 로고
    • Involvement of the jak-3 Janus kinase in signalling by interleukin 2 and 4 in lymphoid and myeloid cells
    • Witthuhn, B. A., Silvennoinen, O., Miura, O., Lai, K. S., Cwik, C., Liu, E. T., Ihle, J. N., Involvement of the Jak-3 Janus kinase in signalling by interleukin 2 and 4 in lymphoid and myeloid cells. Nature 1994. 370: 153-157.
    • (1994) Nature , vol.370 , pp. 153-157
    • Witthuhn, B.A.1    Silvennoinen, O.2    Miura, O.3    Lai, K.S.4    Cwik, C.5    Liu, E.T.6    Ihle, J.N.7
  • 50
    • 0028881246 scopus 로고
    • Mechanism of inhibition of eosinophil activation by transforming growth factor-beta. Inhibition of Lyn, MAP, Jak2 kinases and STAT1 nuclear factor
    • Pazdrak, K., Justement, L. and Alam, R., Mechanism of inhibition of eosinophil activation by transforming growth factor-beta. Inhibition of Lyn, MAP, Jak2 kinases and STAT1 nuclear factor. J. Immunol. 1995. 155: 4454-4458.
    • (1995) J. Immunol. , vol.155 , pp. 4454-4458
    • Pazdrak, K.1    Justement, L.2    Alam, R.3
  • 51
    • 0032080605 scopus 로고    scopus 로고
    • IL-15 enhances the response of human γδ T cells to nonpeptide microbial antigens
    • Garcia, V. E., Jullien, D., Song, M., Uyemura, K., Shuai, K., Morita, C. T. and Modlin, R. I., IL-15 enhances the response of human γδ T cells to nonpeptide microbial antigens. J. Immunol. 1998. 160: 4322-4329.
    • (1998) J. Immunol. , vol.160 , pp. 4322-4329
    • Garcia, V.E.1    Jullien, D.2    Song, M.3    Uyemura, K.4    Shuai, K.5    Morita, C.T.6    Modlin, R.I.7
  • 53
    • 0030049469 scopus 로고    scopus 로고
    • Interleukin-15 induces adhesion receptor redistribution in T lymphocytes
    • Nieto, M., del Pozo, M. A. and Sanchez-Madris, F., Interleukin-15 induces adhesion receptor redistribution in T lymphocytes. Eur. J. Immunol. 1996. 26: 1302-1307.
    • (1996) Eur. J. Immunol. , vol.26 , pp. 1302-1307
    • Nieto, M.1    Del Pozo, M.A.2    Sanchez-Madris, F.3
  • 54
    • 0031571801 scopus 로고    scopus 로고
    • IL-15 functions as a potent autocrine regulator of macrophage proinflammatory cytokine production: Evidence for differential receptor subunit utilization associated with stimulation or inhibition
    • Alleva, D. G., Kaser, S. B., Monroy, M. A., Fenton, M. J. and Beller, D. I., IL-15 functions as a potent autocrine regulator of macrophage proinflammatory cytokine production: evidence for differential receptor subunit utilization associated with stimulation or inhibition. J. Immunol. 1997. 159: 2941-2951.
    • (1997) J. Immunol. , vol.159 , pp. 2941-2951
    • Alleva, D.G.1    Kaser, S.B.2    Monroy, M.A.3    Fenton, M.J.4    Beller, D.I.5
  • 56
    • 0023277545 scopus 로고
    • Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction
    • Chomczynski, P. and Sacchi, N., Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 1987. 162: 156-159.
    • (1987) Anal. Biochem. , vol.162 , pp. 156-159
    • Chomczynski, P.1    Sacchi, N.2


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