MDCK cell cultures as an epithelial in vitro model: Cytoskeleton and tight junctions as indicators for the definition of age-related stages by confocal microscopy

Pharmaceutical Research

Volumn 15, Issue 7, 1998, Pages 964-971

  Rothen Rutishauser, Barbara ^a   Krämer, Stefanie D ^b   Braun, Annette ^a   Günthert, Maja ^a   Wunderli Allenspach, Heidi ^a  

^a ETH ZURICH   (Switzerland)

^b SANOFI   (France)

Author keywords

Age related changes; Confocal laser scanning microscopy; Cytoskeleton; In vitro epithelial cell model; MDCK cells; Tight junctions

Indexed keywords

ACTIN; TUBULIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL PROLIFERATION; CELL STRAIN; CELL STRUCTURE; CELL VOLUME; CONTROLLED STUDY; CYTOSKELETON; DOG; ELECTRIC RESISTANCE; EPITHELIUM CELL; GROWTH CURVE; IMAGE RECONSTRUCTION; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; KIDNEY CELL; LASER MICROSCOPY; MODEL; NONHUMAN; PRIORITY JOURNAL; SIGNAL PROCESSING; TIGHT JUNCTION;

ANIMALS; CELL AGING; CELL DIVISION; CELLS, CULTURED; CYTOSKELETON; DOGS; EPITHELIAL CELLS; FLUORESCENT ANTIBODY TECHNIQUE; KIDNEY; MICROSCOPY, CONFOCAL; TIGHT JUNCTIONS;

EID: 0031824307     PISSN: 07248741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1011953405272     Document Type: Article

References (23)

1. K. L. Audus, R. L. Bartel, I. J. Hidalgo, and R. T. Borchardt. The use of cultured epithelial and endothelial cells for drug transport and metabolism studies. Pharm. Res. 7:435-451 (1990).
2. P. Artursson and R. T. Borchardt. Intestinal drug absorption and metabolism in cell cultures: Caco-2 and beyond. Pharm. Res. 14:1655-1658 (1997).
3. J. A. McRoberts, M. Taub, and M. H. Saier. The Madin Darby canine kidney (MDCK) cell line. In G. Sato (ed.), Functionally Differentiated Cell Lines, Alan R. Liss. Inc., New York, 1981, pp. 117-139.
4. B. Gumbiner. Structure, biochemistry, and assembly of epithelial tight junctions. Am. J. Physiol. 253:749-758 (1987).
5. R. Bacallao, C. Antony, C. Dotti, E. Karsenti, E. H. K. Stelzer, and K. Simons. The subcellular organization of Madin Darby canine kidney cells during the formation of a polarized epithelium. J. Cell Biol. 109:2817-2832 (1989).
6. S. Eskelinen, V. Huotari, R. Sormunen, R. Palovuori, J. W. Kok, and V.-P. Lehto. Low intracellular pH induces redistribution of fodrin and instabilization of lateral walls in MDCK cells. J. Cell. Physiol. 150:122-133 (1992).
7. B. Gumbiner and K. Simons. A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide. J. Cell Biol. 102:457-468 (1986).
8. B. Gumbiner, B. Stevenson, and A. Grimaldi. The role of the cell adhesion molecule uvomorulin in the formation and maintenance of the epithelial junctional complex. J. Cell Biol. 107:1575-1587 (1988).
9. L. Gonzalez-Mariscal, B. Chávez de Ramirez, and M. Cereijido. Tight junction formation in cultured epithelial cells (MDCK). J. Membr. Biol. 86:113-125 (1985).
10. D. Shotton and N. White. Confocal scanning microscopy: three-dimensional biological imaging. Trends Biochem. Sci. 14:435-439 (1989).
11. R. C. Gonzalez and P. Wintz. Digital Image Processing. Addison-Wesley Publishing Company, Reading. 1987.
12. B. M. Rothen-Rutishauser, E. Ehler, E. Perriard, J. M. Messerli, and J.-C. Perriard. Different behaviour of the non-sarcomeric cytoskeleton in neonatal and adult rat cardiomyocytes. J. Mol. Cell. Cardiol. 30:19-31 (1998).
13. M. Schliwa, J. van Blerkom, and K. R. Porter. Stabilization of the cytoplasmic ground substance in detergent-opened cells and a structural and biochemical analysis of its composition. Proc. Natl. Acad. Sci. USA 78:4329-4333 (1981).
14. J. E. Lever. Inducers of mammalian cell differentiation stimulate dome formation in a differentiated kidney epithelial cell line (MDCK). Proc. Natl. Acad. Sci. USA 76:1323-1327 (1979).
15. M. Cereijido, E. S. Robbins, W. J. Dolan, C. A. Rutunno, and D. D. Sabatini. Polarized monolayers formed by epithelial cells on a permeable and translucent support. J. Cell Biol. 77:853-880 (1978).
16. M. J. Cho, D. P. Thompson, C. T. Cramer, T. J. Vidmar, and J. F. Scieszka. The Madin Darby canine kidney (MDCK) epithelial cell monolayer as a model cellular transport barrier. Pharm. Res. 6:71-77 (1989)
17. C. Butor and J. Davoust. Apical to basolateral surface area ratio and polarity of MDCK cells grown on different supports. Exp. Cell Res. 203:115-127 (1992).
18. F. Cao and J. M. Burke. Protein insolubility and late-stage morpho-genesis on long-term postconfluent cultures of MDCK epithelial cells. Biochem. Biophys. Res. Comm. 234:719-728 (1997).
19. J. D. Valentich. Morphological similarities between the dog kidney cell line MDCK and the mammalian cortical collecting tubule. Ann. N. Y. Acad. Sci. 372:384-405 (1981).
20. M. G. Farquhar and G. E. Palade. Junctional complexes in various epithelia. J. Cell Biol. 17:375-412 (1963).
21. L. A. Jesaitis and D. A. Goodenough. Molecular characterization and tissue distribution of ZO-2. a tight junction protein homologous to ZO-1 and the Drosophila discs-large tumor supressor protein. J. Cell Biol. 124:949-961 (1994).
22. J. E. Lever. Variant (MDCK) kidney epithelial cells altered in response to inducers of dome formation and differentiation. J. Cell Physiol. 122:45-52 (1985).
23. M. M. Jaeger, V. Dodane, and B. Kachar. Modulation of tight junction morphology and permeability by an epithelial factor. J. Membr. Biol. 139:41-48 (1994).