High-level expression, purification, kinetic characterization and crystallization of protein farnesyltransferase β-subunit C-terminal mutants

Protein Engineering

Volumn 12, Issue 4, 1999, Pages 341-348

  Wu, Zhen ^a   Demma, Mark ^a   Strickland, Corey L ^a   Syto, Rosalinda ^a   Le, Hung V ^a   Windsor, William T ^a   Weber, Patricia C ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Crystallization; High level expression; Protein farnesyltransferase; SPA assay; subunit C terminal mutants

Indexed keywords

PROTEIN FARNESYLTRANSFERASE;

ARTICLE; BACTERIUM CULTURE; CARBOXY TERMINAL SEQUENCE; CISTRON; CRYSTALLIZATION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SUBUNIT; ESCHERICHIA COLI; MICHAELIS CONSTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; STEADY STATE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FELIS CATUS;

EID: 0032949384     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/12.4.341     Document Type: Article

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