Disruption of the YRB2 gene retards nuclear protein export, causing a profound mitotic delay, and can be rescued by overexpression of XPO1/CRM1

Journal of Biochemistry

Volumn 125, Issue 3, 1999, Pages 574-585

  Noguchi, Eishi ^a   Saitoh, Yoh Hei ^a   Sazer, Shelley ^b   Nishimoto, Takeharu ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Mitotic delay; Nuclear export; XPO1 CRM1; YRB1 2

Indexed keywords

BINDING PROTEIN; NUCLEAR PROTEIN;

ARTICLE; CHROMOSOME LOSS; COLD SENSITIVITY; COLONY FORMATION; GENE DISRUPTION; GENE OVEREXPRESSION; MITOSIS; MITOSIS INHIBITION; NONHUMAN; PROTEIN DEPLETION; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

NES; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 0032941773     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022323     Document Type: Article

References (65)

1. Belhumeur, P., Lee, A., Tam, R., DiPaolo, T., Fortin, N., and Clark, M.W. (1993) GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization. Mol. Cell. Biol. 13, 2152-2161
2. Bischoff, F.R., Krebber, H., Kempf, K., Hermes, I., and Ponstingl, H. (1995) Human RanGTPase-activating protein RanGAP1 is homologue of yeast Rnalp involved in mRNA processing and transport. Proc. Natl. Acad. Sci. USA 92, 1749-1753
3. Becker, J., Melchior, F., Gerke, V., Bischoff, F.R., Ponstingl, H., and Wittinghofer, A. (1995) RNA1 encodes a GTPase-activating protein specific for Gsp1, the Ran/TC4 homologue of Saccharomyces cerevisiae. J. Biol. Chem. 270, 11860-11865
4. Bischoff, F.R. and Ponstingl, H. (1991) Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature 354, 80-82
5. Ohtsubo, M., Ozaki, H., and Nishimoto, T. (1989) The RCC1 protein, a regulator for the onset of chromosome condensation locates in the nucleus and bind to DNA. J. Cell Biol. 109, 1389-1397
6. Frasch, M. (1991) The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene regulator of chromatin condensation, RCC1. EMBO J. 10, 1225-1236
7. Matynia, A., Dimitrov, K., Mueller, U., He, X., and Sazer, S. (1996) Perturbations in the spilp GTPase cycle of Schizosaccharomyces pombe through its GTPase-activating protein and guanine nucleotide exchange factor components result in similar phenotypic consequences. Mol. Cell Biol. 16, 6352-6362
8. Matunis, M.J., Coutavas, E., and Blobel, G. (1996) A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J. Cell Biol. 135, 1457-1470
9. Mahajan, R., Delphin, C., Guan, T., Gerace, L., and Melchior, F. (1997) A small ubiquitin related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2. Cell 88, 97-107
10. Görlich, D. and Mattaj, I.W. (1996) Nucleocytoplasmic transport. Science 271, 1513-1518
11. Nigg, E.A. (1997) Nucleocytoplasmic transport: signals, mechanism and regulation. Nature 386, 779-787
12. Weis, K. (1998) Importins and exporting: how to get in and out of the nucleus. Trends Biochem. Sci. 23, 185-189
13. Melchior, F. and Gerace, L. (1998) Two-way trafficking with Ran. Trends Cell Biol. 8, 175-179
14. Wozniak, R., Rout, M.P., and Aitchison, J.D. (1998) Karyopherins and Kissing cousins. Trends Cell Biol. 8, 184-188
15. Dingwall, C.D., Kandels-Lewis, S., and Seraphin, B. (1995) A family of Ran binding proteins that includes nucleoporins. Proc. Natl. Acad. Sci. USA 92, 7525-7529
16. Hartman, E. and Görlich, D. (1995) A Ran-binding motif in nuclear pore proteins. Trends Cell Biol. 5, 192-193
17. Görlich, D., Dabrowski, M., Bischoff, F.R., Kutay, U., Bork, P., Hartmann, E., Prehn, S., and Izaurralde, E. (1997) A novel class of RanGTP binding proteins. J. Cell Biol. 138, 65-80
18. Ullman, K.S., Powers, M.A., and Forbes, D.J. (1997) Nuclear export receptors: from Importin to exportin. Cell 90, 967-970
19. Coutavas, E., Ren, M., Oppenheim, J.D., D'Eustachio, P., and Rush, M.G. (1993) Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. Nature 366, 585-587
20. Ouspenski, I.L., Mueller, U.W., Matynia, A., Sazer, S., Elledge, S.J., and Brinkley, B.R. (1995) Ran-binding protein-1 is an essential component of the Ran/RCC1 molecular switch system in budding yeast. J. Biol. Chem. 270, 1975-1978
21. Hayashi, N., Yokoyama, N., Seki, T., Azuma, Y., Ohba, T., and Nishimoto, T. (1995) RanBP1, a Ras-like nuclear G-protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4. Mol. Gen. Genet. 247, 661-669
22. Yokoyama, N., Hayashi, N., Seki, T., Pante, N., Ohba, T., Nishii, K., Kuma, K., Hayashida, T., Miyata, T., Aebi, U., Fukui, M., and Nishimoto, T. (1995) A giant nucleopore protein that binds Ran/TC4. Nature 376, 184-188
23. Wu, J., Matunis, M.J., Kraemer, D., Blobel, G., and Coutavas, E. (1995) Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J. Biol. Chem. 270, 14209-14213
24. Melchior, F., Guan, T., Yokoyama, N., Nishimoto, T., and Gerace, L. (1995) GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import. J. Cell Biol. 131, 571-581
25. Mueller, L., Cordes, V.C., Bischoff, F.R., and Ponstingl, H. (1998) Human RanBP3, a group of nuclear RanGTP binding proteins. FEBS Lett. 427, 330-336
26. Schlenstedt, G., Wong, D.H., Koepp, D., and Silver, P.A. (1995) Mutants in a yeast Ran binding protein are defective in nuclear transport. EMBO J. 14, 5367-5378
27. Noguchi, E., Hayashi, N., Nakashima, N., and Nishimoto, T. (1997) Yrb2p, Nup2p-related yeast protein has functional overlap with Rna1p, yeast RanGAP protein. Mol. Cell. Biol. 17, 2235-2246
28. He, X., Hayashi, N., Walcott, N.G., Azuma, Y., Patterson, T.E., Bischoff, F.R., Nishimoto, T., and Sazer, S. (1998) The identification of cDNAs that affect the mitosis-to-interphase transition in Schizosaccharomyces pombe, including sbp1, which encodes a spi1p-GTP-binding protein. Genetics 148, 645-656
29. Richards, S., Lounsbury, K.M., Carey, K.L., and Macara, I.G. (1996) A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1. J. Cell Biol. 134, 1157-1168
30. Taura, T., Schlenstedt, G., and Silver, P.A. (1997) Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue. J. Biol. Chem. 272, 31877-31884
31. Turi, T.G., Mueller, U.W., Sazer, S., and Rose, J.K. (1996) Characterization of a nuclear protein conferring brefeldin A resistance in Schizosaccharomyces pombe. J. Biol. Chem. 271, 9166-9171
32. Schlenstedt, G., Smirnova, E., Deane, R., Solsbacher, J., Kutay, U., Gorlich, D., Ponstingl, H., and Bischoff, F.R. (1997) Yrb4p, a yeast Ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus. EMBO J. 16, 6237-6249
33. Chi, N., Adam, J.H., Visser, G.D., and Adam, S.A. (1996) RanBP1 stabilizes the interaction of Ran with p97 in nuclear protein import. J. Cell Biol. 135, 559-569
34. Lounsbury, K. and Macara, I.G. (1997) Ran-binding protein 1 (RanBP1) form a ternary complex with Ran and Karyopherin β and reduces Ran GTPase-activating protein (RanGAP) inhibition by Karyopherin β. J. Biol. Chem. 272, 551-555
35. Bischoff, F.R. and Görlich, D. (1997) RanBP1 is crucial for the release of RanGTP from importin β-related nuclear transport factors. FEBS Lett. 419, 249-254
36. Hutter, K.J. and Eipel, H.E. (1979) Microbial determinations by flow cytometry. J. Gen. Microbiol. 113, 369-375
37. Hagan, I.M. and Hyams, J.S. (1988) The use of cell division cycle mutants to investigate the control of microtubule distribution in fission yeast Schizosaccharomyces pombe. J. Cell Sci. 89, 343-357
38. Woods, A., Sherwin, T., Sasse, R., MacRAE, T.H., Baines, A.J., and Gull, K. (1989) Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies. J. Cell Sci. 93, 491-500
39. Stade, K., Ford, C.S., Guthrie, C., and Weis, K. (1997) Exportin (Xpo1p/XPO1/CRM1p) is an essential nuclear export factor. Cell 90, 1041-1050
40. Hayashi, N., Seino, H., Irie, K., Watanabe, M., Clark, K.L., Matsumoto, K., and Nishimoto, T. (1996) Genetical interaction of DED1 encoding a putative ATP-dependent RNA helicase with SRM1 encoding a mammalian RCC1 homologue in Saccharomyces cerevisiae. Mol. Gen. Genet. 253, 149-156
41. Winston, F., Chumley, F., and Fink, G.R. (1983) Eviction and transplacement of mutant genes in yeast. Methods Enzymol. 101, 211-228
42. Yu-Sherman, M. and Goldberg, A.L. (1992) Involvement of the chaperonin dnaK in the rapid degradation of a mutant protein in Escherichia coli. EMBO J. 11, 71-77
43. Dasso, M., Seki, T., Azuma, Y., Ohba, T., and Nishimoto, T. (1994) A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation. EMBO J. 13, 5732-5744
44. Azuma, Y., Hachiya, T., and Nishimoto, T. (1997) Inhibition by anti-Rcc1 monoclonal antibodies of Rcc1-stimulated guanine nucleotide exchange on Ran GTPase. J. Biochem. 122, 1133-1138
45. Surana, U., Robitsch, H., Price, C., Schuster, T., Fitch, I., Futcher, A.B., and Nasmyth, K. (1991) The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell 65, 145-161
46. Cross, F., Hartwell, L.H., Jackson, C.,and Konopka, J. B. (1988) Conjugation in Saccharomyces cerevisiae. Annu. Rev. Cell Biol. 4, 429-457
47. Yeh, E., Skibbens, R.V., Cheng, J.W., Salmon, E.D., and Bloom, K. (1995) Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae. J. Cell Biol. 130, 687-700
48. Hoyt, M.A., Totis, L., and Roberts, B.T. (1991) S. cerevisiae genes required for cell-cycle arrest in response to loss of microtubule function. Cell 66, 507-517
49. Li, R. and Murray, A.W. (1991) Feedback control of mitosis in budding yeast. Cell 66, 519-531
50. Kitada, K., Johnson, A.L., Johnston, L.H., and Sugino, A. (1993) A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5. Mol. Cell Biol. 13, 4445-4457
51. Gietz, R.D. and Sugino, A. (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base restriction sites. Gene 74, 527-534
52. Broach, J.R., Strathern, J.N., and Hicks, J.B. (1979) Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8, 121-133
53. Sikorski, R.S. and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27
54. Fornerod, M., Ohno, M., Yoshida, M., and Mattaj, I .W. (1997) XPO1/CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 90, 1051-1060
55. Taura, T., Krebber, H., and Silver, P.A. (1998) A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export. Proc. Natl. Acad. Sci. USA 95, 7427-7432
56. Kellogg, D.R., Kikuchi, A., Fujii-Nakata, T., Turck, C.W., and Murray, A.W. (1995) Members of NAP/SET family of proteins interact specifically with B-type cyclins. J. Cell Biol. 130, 661-673
57. Kellogg, D.R. and Murray, A.W. (1995) NAP1 acts with Clb2 to perform mitotic functions and to suppress polar bud growth in budding yeast. J. Cell Biol. 130, 675-685
58. Sikder, H., Funakoshi, M., Nishimoto, T., and Kobayashi, H. (1997) An altered nuclear migration into the daughter bud is induced by the cyclin A1-mediated Cdc28 kinase through an aberrant spindle movement in Saccharomyces cerevisiae. Cell Struct. Funct. 22, 465-476
59. Hagting, A., Karlsson, C., Clute, P., Jackman, M., and Pines, J. (1998) MPF localization is controlled by nuclear export. EMBO J. 17, 4127-4138
60. Loeb, J.D.J., Schlenatedt, G., Pellman, D., Komitzer, D., Silver, P.A., and Fink, G.R. (1995) The yeast nuclear import receptor is required for mitosis. Proc. Natl. Acad. Sci. USA 92, 7647-7651
61. Kutay, U., Izaurralde, E., Bischoff, F.R., Mattaj, I.W., and Görlich, D. (1997) Dominant-negative mutants of importin-β block multiple pathways of import and export through the nuclear pore complex. EMBO J. 16, 1153-1163
62. Irniger, S., Piatti, S., Michaelis, C., and Nasmyth, K. (1995) Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast. Cell 81, 269-277
63. Matsuzaki, H., Nakajirna, R., Nishiyama, J., Araki, H., and Oshima, Y. (1990) Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of yeast plasmid. J. Bacteriol. 172, 610-618
64. Tanaka, K., Matsumoto, K., and Toh-e, A. (1988) Dual regulation of the polyubiquitin gene by cyclic AMP and heat shock in yeast. EMBO J. 7, 495-502
65. Oki, M., Noguchi, E., Hayashi, N., and Nishimoto, T. (1998) Nuclear protein import, but not mRNA export, is defective in all Saccharomyces cerevisiae mutants that produce temperature-sensitive forms of the Ran GTPase homologue Gsp1p. Mol. Gen. Genet. 257, 624-634