Recognition of 2'-deoxyisoinosine triphosphate by the Klenow fragment of DNA polymerase I

Nucleosides and Nucleotides

Volumn 18, Issue 3, 1999, Pages 403-410

  Beaussire, Jean Jacques ^a   Pochet, Sylvie ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

2' DEOXYISOINOSINE TRIPHOSPHATE; 5 METHYL 2 DEOXYISOCYTIDINE; DEOXYINOSINE; DNA DIRECTED DNA POLYMERASE BETA; EXONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; BASE PAIRING; DNA TEMPLATE; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR RECOGNITION; SYNTHESIS;

EID: 0032923949     PISSN: 07328311     EISSN: None     Source Type: Journal    
DOI: 10.1080/15257779908043085     Document Type: Article

References (25)

1. Piccirilli, J. A.; Krauch, T.; Moroney, S. E.; Benner, S. A. Nature 1990, 343, 33-37.
2. Switzer, C. Y.; Moroney, S. E.; Benner, S. A. Biochemistry 1993, 32, 10489-10496.
3. Horlacher, J.; Hottiger, M.; Podust, V. N.; Hubscher, U.; Benner, S. A. Proc. Natl. Acad. Sci. USA 1995, 92, 6329-33.
4. Schweitzer, B. A.; Kool, E. T. J. Org. Chem. 1994, 59, 7238-7242.
5. Moran, S.; Ren, R. X.-F.; Kool, E. T. Proc. Natl. Acad. Sci. USA 1997, 94, 10506-10511.
6. Loakes, D.; Brown, D. M.; Linde, S.; Hill, F. Nucleic Acids Res. 1995, 23, 2361-2366.
7. Bergstrom, D. E.; Zhang, P.; Toma, P. H.; Andrews, P. C.; Nichols, R. J. Am. Chem. Soc. 1995, 117, 1201-1209.
8. Zaccolo, M.; Williams, D. M.; Brown, D. M.; Gherardi, E. J. Mol. Biol. 1996, 255, 589-603.
9. Sala, M.; Pezo, V.; Pochet, S.; Wain-Hobson, S. Nucleic Acids Res. 1996, 24, 3302-3306.
10. Hoops, G. C.; Zhang, P.; Johnson, W. T.; Natasha; Paul; Bergstrom, D. E.; Davisson, V. J. Nucleic Acids Res. 1997, 25, 4866-4871.
11. Bailly, C.; Waring, M. J. Nucleic Acids Res. 1998, 26, 4309-4314.
12. Beaussire, J.-J.; Pochet, S. Nucl. & Nucl. 1995, 14, 805-808.
13. Beaussire, J.-J.; Pochet, S. Tetrahedron 1998, 54, 13547-13556.
14. Sepiol, J.; Kazimierczuk, Z.; Shugar, D. Z. Naturforsh. (C) 1976, 31, 361-370.
15. Seela, F.; Chen, Y. Nucleic Acids Res. 1995, 23, 2499-2505.
16. Tener, G. M. J. Am. Chem. Soc. 1961, 83, 159-168.
17. 2O) : 0.23.
18. 2O) : 2.1.
19. 2O) : 8.1.
20. Moffatt, J. G. Can. J. Chem. 1964, 42, 599-604.
21. 2O) : -21.04 (t, J = 16 Hz); -10.29 (d); -5.30 (d); MS (FAB-) : 491 (M-H)-.
22. -, Biolabs). The second (5 μL) contained the triphosphates (in the range of 100 nM to 1 mM). The mixture was incubated at 23°C for 20 min.. Reactions were stopped by addition of 2.5 μL of stop buffer, heated at 85°C for 2 min. then chilled on ice prior to loading on polyacrylamide gel (20%, 7M urea).
23. As described in 22, excepted that the hybrid concentration was 165 nM (with primer/template ratio of 1/1), and the polymerase was 0.16 U/μL.
24. Lutz, M. J.; Horlacher, J.; Benner, S. A. Bioorg. Med. Chem. Lett. 1998, 8, 499-504.
25. Roberts, C.; Bandaru, R.; Switzer, C. J. Am. Chem. Soc. 1997, 119, 4640-4649.