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note
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The free energy difference for the U/G pair in the nonamer duplex (entry 18, Table 2) appears to be anomalously large (4.2 kcal/mol). It is not clear at this time why this is so, but further investigation did show that inverting the U/G pair to G/T (entry 15) yielded a free energy difference similar to that obtained in the dodecamer duplex for U/G (3.0 vs 2.5 kcal/ mol, entry 7 vs 15). The fact that the free energy differences between the standard Watson-Crick pairs GIC and A/U (entries 11 and 13) is as expected, serves as a control for the integrity of the G and U bearing oligomers used for entry 18 of Table 2.
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