Spacing of promoter elements regulates the basal expression of the soxS gene and converts SoxR from a transcriptional activator into a repressor

EMBO Journal

Volumn 16, Issue 5, 1997, Pages 1056-1065

  Hidalgo, Elena ^a   Demple, Bruce ^a  

^a HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

DNA binding; Escherichia coli promoter; Oxidative stress; RNA polymerase; Signal transduction

Indexed keywords

NITRIC OXIDE; PARAQUAT; PROTEIN; PROTEIN SOXR; RNA POLYMERASE; SUPEROXIDE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BINDING AFFINITY; CONSENSUS SEQUENCE; CONTROLLED STUDY; ESCHERICHIA; ESCHERICHIA COLI; GENE ACTIVATION; GENE DELETION; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; GENE REPRESSION; NONHUMAN; OXIDATION REDUCTION REACTION; OXIDATIVE STRESS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION;

BACTERIAL PROTEINS; BASE SEQUENCE; BETA-GALACTOSIDASE; CLONING, MOLECULAR; DNA-BINDING PROTEINS; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXIDATIVE STRESS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; SEQUENCE DELETION; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

ESCHERICHIA; ESCHERICHIA COLI;

EID: 0031041391     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.5.1056     Document Type: Article

References (40)

1. Amábile-Cuevas, C.F. and Demple, B. (1991) Molecular characterization of the soxRS genes of Escherichia coli: two genes control a superoxide stress regulon. Nucleic Acids Res., 19, 4479-4484.
2. Ansari, A.Z., Chael, M.L. and O'Halloran, T.V. (1992) Allosteric under-winding of DNA is a critical step in positive control of transcription by Hg-MerR. Nature, 355, 87-89.
3. Ansari, A.Z., Bradner, J.E. and O'Halloran, T.V. (1995) DNA-bend modulation in a repressor-to-activator switching mechanism. Nature, 374, 371-375.
4. Collado-Vides, J., Magasanik, B. and Gralla, J.D. (1991) Control site location and transcriptional regulation in Escherichia coli. Microbiol. Rev., 55, 371-394.
5. Frantz, B. and O'Halloran, T.V. (1990) DNA distortion accompanies transcriptional activation by the metal-responsive gene-regulatory protein MerR. Biochemistry, 29, 4747-4751.
6. Gralla, J.D. and Collado-Vides, J. (1996) Organization and function of transcription regulatory elements. In Neidhardt, F.C. et al. (eds), Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. Washington, DC, pp. 1232-1245.
7. Greenberg, J.T., Monach, P., Chou, J.H., Josephy, P.D. and Demple, B. (1990) Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. Proc. Natl Acad. Sci. USA, 87, 6181-6185.
8. Hawley, D.K. and McClure, W.R. (1983) Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res., 11, 2237-2255.
9. Heltzel, A., Lee, I.W., Totis, P.A. and Summers, A.O. (1990) Activator-dependent preinduction binding of sigma-70 RNA polymerase at the metal-regulated mer promoter. Biochemistry, 29, 9572-9584.
10. Hidalgo, E. and Demple, B. (1994) An iron-sulfur center essential for transcriptional activation by the redox-sensing SoxR protein. EMBO J., 13, 138-146.
11. Hidalgo, E. and Demple, B. (1996a) Activation of SoxR-dependent transcription in vitro by noncatalytic or NifS-mediated assembly of [2Fe-2S] clusters into apo-SoxR. J. Biol. Chem., 271, 7269-7272.
12. Hidalgo, E. and Demple, B. (1996b) Adaptive responses to oxidative stress: the soxRS and oxyR regulons. In Lin, E.C.C. and Lynch, A.S. (eds), Regulation of Gene Expression in Escherichia coli. R.G. Landes Co., Austin, TX, pp. 433-450.
13. Hidalgo, E., Bollinger, J.M., Jr, Bradley, T.M., Walsh, C.T. and Demple, B. (1995) Binuclear [2Fe-2S] clusters in the Escherichia coli SoxR protein and role of the metal centers in transcription. J. Biol. Chem., 270, 20908-20914.
14. Jaurin, B., Grundström, T. and Normark, S. (1982) Sequence elements determining ampC promoter strength in E.coli. EMBO J., 1, 875-881.
15. Lanzer, M. and Bujard, H. (1988) Promoters largely determine the efficiency of repressor action. Proc. Natl Acad. Sci. USA, 85, 8973-8977.
16. Lopilato, J., Bortner, S. and Beckwith, J. (1986) Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasimd copy number of pBR322 and its derivatives. Mol. Gen. Genet., 205, 285-290.
17. Lund, P.A. and Brown, N.L. (1989) Up-promoter mutations in the positively-regulated mer promoter of Tn501. Nucleic Acids Res., 17, 5517-5527.
18. Miller, J.H. (1992) A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Nunoshiba, T. and Demple, B. (1993) Potent intracellular oxidative stress exerted by the carcinogen 4-nitroquinoline-N-oxide. Cancer Res., 53, 3250-3252.
20. Nunoshiba, T., Hidalgo, E., Amábile-Cuevas, C.F. and Demple, B. (1992) Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the soxS regulatory gene. J. Bacteriol., 174, 6054-6060.
21. Nunoshiba, T., DeRojas-Walker, T., Wishnok, J.S, Tannenbaum, S.R. and Demple, B. (1993) Activation by nitric oxide of an oxidative-stress response that defends Escherichia coli against activated macrophages. Proc. Natl Acad. Sci. USA, 90, 9993-9997.
22. Parkhill, J. and Brown, N.L. (1990) Site-specific insertion and deletion mutants in the mer promoter-operator region of Tn501; the nineteen base-pair spacer is essential for normal induction of the promoter by MerR. Nucleic Acids Res., 18, 5157-5162.
23. Pivec, L., Rozkot, F., Sázelová, P. and Vítek, A. (1985) Consensus symmetry pattern in E.coli promoter sequences. Folia Biol., 31, 213-234.
24. Podkovyrov, S.M. and Larson, T.J. (1995) A new vector - host system for construction of lacZ transcriptional fusions where only low-level gene expression is desirable. Gene, 156, 151-152.
25. Ross, W., Park, S.-J. and Summers, A.O. (1989) Genetic analysis of transcriptional activation and repression in the Tn21 mer operon. J. Bacteriol., 171, 4009-4018.
26. Ross, W., Gosink, K.K., Salomon, J., Igarashi, K., Zou, C., Ishihama, A., Severinov, K. and Gourse, R.L. (1993) A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase. Science, 262, 1407-1413.
27. Rossi, J.J., Soberon, X., Marumoto, Y., McMahon, J. and Itakura, K. (1983) Biological expression of an Escherichia coli consensus sequence promoter and some mutant derivatives. Proc. Natl Acad. Sci. USA, 80, 3203-3207.
28. Russell, D.R. and Bennett, G.N. (1982) Construction and analysis of in vivo activity of E.coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing. Gene, 20, 231-243.
29. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd. edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
30. Siebenlist, U., Simpson, R.B. and Gilbert, W. (1980) E.coli RNA polymerase interacts homologously with two different promoters. Cell, 20, 269-281.
31. Silhavy, T.J., Berman, M.L. and Enquist, L.W. (1984) Experiments with Gene Fusions. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
32. Simons, R.W., Houman, F. and Kleckner, N. (1987) Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene, 53, 85-96.
33. s promoter. Proc. Natl Acad. Sci. USA, 79, 1069-1072.
34. Summers, A.O. (1992) Untwist and shout: a heavy metal-responsive transcriptional regulator. J. Bacteriol., 174, 3097-3101.
35. Tsaneva, I.R. and Weiss, B. (1990) soxR, a locus governing a superoxide response regulon in Escherichia coli K-12. J. Bacteriol., 172, 4197-4205.
36. Werel, W., Schickor, P. and Heumann, H. (1991) Flexibility of the DNA enhances promoter affinity of Escherichia coli RNA polymerase. EMBO J., 10, 2589-2594.
37. Wu, J. and Weiss, B. (1991) Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of Escherichia coli. J. Bacteriol., 173, 2864-2871.
38. Wu, J. and Weiss, B. (1992) Two-stage induction of the soxRS (superoxide response) regulon of Escherichia coli. J. Bacteriol., 174, 3915-3920.
39. Wu, J., Dunham, W.R. and Weiss, B. (1995) Overproduction and physical characterization of SoxR, a [2Fe-2S] protein that governs an oxidative stress response regulon in Escherichia coli. J. Biol. Chem., 270, 10323-10327.
40. Xu, F. and Cohen, S.N. (1995) RNA degradation in Escherichia coli regulated by 3′ adenylation and 5′ phosphorylation. Nature, 374, 180-183.