Expression, purification and DNA-cleavage activity of recombinant 68-kDa human topoisomerase I-target for antitumor drugs

Anticancer Research

Volumn 19, Issue 1 A, 1999, Pages 317-327

  Bronstein, Igor B ^a   Wynne Jones, Abigail ^a   Sukhanova, Alyona ^b   Fleury, Fabrice ^c   Ianoul, Anatoli ^b,c   Holden, Joseph A ^d   Alix, Alain J P ^c   Dodson, Guy G ^a   Jardillier, Jean Claude ^c   Nabiev, Igor ^c   Wilkinson, Anthony J ^a  

^a UNIVERSITY OF YORK   (United Kingdom)

^b SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^c UNIVERSITÉ DE REIMS CHAMPAGNE ARDENNE   (France)

^d UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

Baculovirus system, flow linear dichroism; DNA binding; Domain structure; Expression; Human topoisomerase I; Limited proteolysis; Purification

Indexed keywords

ANTINEOPLASTIC AGENT; DNA TOPOISOMERASE; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACULOVIRUS; DNA CLEAVAGE; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN DEGRADATION; YEAST;

ANIMALS; ANTINEOPLASTIC AGENTS; DNA; DNA TOPOISOMERASES, TYPE I; ENZYME INHIBITORS; ESCHERICHIA COLI; HUMANS; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SPODOPTERA;

EID: 0032897721     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Wang J: DNA topoisomerases. Annu Rev Biochem 65: 635-692, 1996.
2. Hsiang Y, Hertzberg S, Hecht S., Liu L: Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I. J Biol Chem 260: 14873-14878, 1985.
3. Verschraegen C, Levy T, Kudelka A, Llerena E., Ende K, Freedman R. et al: Phase II study of irinotecan in prior chemotherapy-treated squamous cell carcinoma of the cervix. J Clin Oncol 15: 625-631, 1997.
4. Eng W, Pandit S., Sternglanz R: Mapping of the active site tyrosine of eukaryotle DNA topoisomerase I. J Biol Chem 264: 13373-13376, 1989.
5. Hsiang Y, Lihou M, Liu L: Arrest of replication forks by drug-stabilized topoisomerase I-DNA cleavable complexes as a mechanism of cell killing by camptothecin. Cancer Res 49: 5077-5082, 1989.
6. Liu L and Miller K: Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HcLa cell nuclei. Proc Natl Acad Sci USA 78: 3487-3491, 1981.
7. Stewart L, Ireton G, Parker L, Madden K and Champoux J: Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J Biol Chem 271: 7593-7601, 1996.
8. Stewart L, Ireton G and Champoux J: The domain organization of human topoisomerase I. J Biol Chem 271: 7602-7608, 1996.
9. Redinbo MR, Stewart L, Kuhn P, Champoux JJ, Hol WGJ: Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA. Science 279: 1504-1513, 1998.
10. Stewart L, Redinbo MR, Qiu X, Hol WGJ, Champoux JJ: A model for the mechanism of human topoisomerasel. Science 279: 1534-1541, 1998.
11. Ianoul A, Fleury F, Kryukov E, Sukhanova A, Alix AJP, Wynne-Jones A, Bronstein IB, Kudelina IA, Feofanov A., Berjot M, Maizieres M, Jardillier JC, Dodson GG, Wilkinson AJ and Nabiev I: Raman and CD spectroscopy of recombinant 68-kDa human DNA topoisomcrase I and its complex with suicide DNA-substrate. Biochemistry 37: 14630-14642, 1998.
12. Bronshtein IB, Shuster AM, Shevchenko LV, Gromova II, Kvashuk OA, Geva ON, Gabibov AG: Topoisomerase I from Human placenta - functional-activity of products of expression of cloned cDNA fragments. Mol Biol (Russian) 23: 1222-1225, 1989.
13. 12a Florell S, Martinchich J, Holden J: Purification of DNA topoisomerase I from the spleen of a patient with non-Hodgkin's lymphoma. Anticancer Res 16: 3467-3474, 1997.
14. Christianson T, Sikorski R, Dante M, Shero J, Hieter P: Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992.
15. Gromova I, Buchman V, Abagyan R, Ulyanov A, Bronstein I: Sequence dependent modulating effect of camptothecin on the DNA-cleaving activity of the calf thymus type I topoisomerase. Nucleic Acid Res 18: 637-645, 1990.
16. Bronstein I, Vorobyev S, Timofeev A, Jolles C, Alder S, Holden J: Elevations of DNA topoisomerase I catalytic activity and immunoprotein in human malignancies. Oncology Res 8: 17-25, 1996.
17. Gololobov G, Chernova E, Schourov D, Smirnov I, Kudelina I, Gabibov A: Cleavage of supercoiled plasmid DNA by autoantibody Fab fragment: application of the flow linear dichroism technique. Proc Natl Acad Sci USA 92: 254-257, 1995.
18. Rubin E, Pantazis P, Bharti A, Toppmeyer D, Giovanella B, Kufe D: Identification of a mutant human topoisomerase I with intact catalytic activity and resistance to 9-nitro-camptothecin. J Biol Chem 269: 2433-2439, 1994.
19. Gatto B, Sanders MM, Yu C, Wu HY, Makhey D, La Voi EJ, Liu LF: Identification of topoisomerase I as the cytotoxic target of the protoberberine alkaloid coralyne. Cancer Res 56: 2795-2800, 1996.
20. Svejstrup J, Christiansen K, Andersen A, Lund K, Westergaard O: Minimal DNA duplex requirements for topoisomerase I-mediated cleavage in vitro. J Biol Chem 265: 12529-12535, 1990.
21. Bronstein I, Gromova I, Razin S: Specific cleavage of chicken alpha A-globin and human c-Ha-ras genes by two molecular forms of calf thymus topoisomerase I. Mol Cell Biochem 101: 115-124, 1991.
22. Jensen AD and Svejstrup JQ: Purification and characterisation of human topoisomerase I mutants. Eur J Biochem 236: 389-394, 1996.
23. Alsner J, Svejstrup J, Kjeldsen E, Sorensen B, Westergaard O: Identification of an N-terminal domain of eukaryotic DNA topoisomerase I dispensable for catalytic activity but essential for in vivo function. J Biol Chem 267: 12408-12411, 1992.
24. Gromova I, Kjeldsen E, Sveistrup J, Alsner J, Christiansen K, Westergaard O: Characterization of an altered DNA catalysis of a camptothecin-resistant eukaryotic topoisomerase I. Nucleic Acid Res 21: 593-600, 1993.