Purification and catalytic properties of human caspase family members

Cell Death and Differentiation

Volumn 6, Issue 4, 1999, Pages 362-369

  Garcia Calvo, Margarita ^a   Peterson, Erin P ^a   Rasper, Dita M ^b   Vaillancourt, John P ^b   Zamboni, Robert ^b   Nicholson, Donald W ^b   Thornberry, Nancy A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

Apoptosis; Caspase; Inflammation; Protease

Indexed keywords

CALCIUM; CASPASE; CASPASE 3; CYSTEINE PROTEINASE; ENZYME INHIBITOR; INTERLEUKIN 1BETA CONVERTING ENZYME; PROTEIN; SODIUM CHLORIDE; TETRAPEPTIDE;

APOPTOSIS; ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; FLUOROMETRY; INFLAMMATION; ION EXCHANGE CHROMATOGRAPHY; MICHAELIS CONSTANT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FOLDING; SOLUBILITY;

ESCHERICHIA COLI; FELIS CATUS; MAMMALIA;

EID: 0032891964     PISSN: 13509047     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cdd.4400497     Document Type: Article

References (28)

1. Thornberry NA, Rano TA, Peterson EP, Rasper DM, Timkey T, Garcia-Calvo M, Houtzager VM, Nordstrom PA, Roy S, Vaillancourt JP, Chapman KT and Nicholson DW (1997) A combinatorial approach defines specificities of members of the caspase family and granzyme B. J. Biol. Chem. 272: 17907-17911
2. Ghayur T, Banerjee S, Hugunin M, Butler D, Herzog L, Carter A, Quintal L, Sekut L, Talanian R, Paskind M, Wong W, Kamen R, Tracey D and Allen H (1997) Caspase-1 processes IFN-γ-inducing factor and regulates LPS-induced IFN-γ production. Nature 386: 619-623
3. Gu Y, Kuida K, Tsutsui H, Ku G, Hsiao K, Fleming MA, Hayashi N, Higashino K, Okamura H, Nakanishi K, Kurimoto M, Tanimoto T, Flavell RA, Sato V, Harding MW, Livingston DJ and Su MS-S (1997) Activation of interferon-γ inducing factor mediated by interleukin-1β converting enzyme. Science 275: 206-209
4. Kuida K, Lippke JA, Ku F, Harding MW, Livingston DJ, Su MS-S and Flavell RA (1995) Altered cytokine export and apoptosis in mice deficient in interleukin-1β converting enzyme. Science 267: 2000-2003
5. Li P, Allen H, Banerjee S, Franklin S, Herzog L, Johnston C, McDowell J, Paskind M, Rodman L, Salfeld J, Towne E, Tracey D, Wardwell S, Wei F-Y, Wong W, Kamen R and Seshadri T (1995) Mice deficient in IL-1β-converting enzyme are defective in production of mature IL-1β and resistant to endotoxic shock. Cell 80: 401-411
6. Kuida K, Zheng TS, Na S, Kuan C, Yang D, Karasuyama H, Rakic P and Flavell RA (1996) Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature 384: 368-372
7. Thornberry N and Lazebnik Y (1998) Caspases: Enemies within. Science 281: 1312-1316
8. Howard AD, Kostura MJ, Thornberry N, Ding GJF, Limjuco G, Weidner J, Salley JP, Hogquist KA, Chaplin DD, Mumford RA, Schmidt JA and Tocci MJ (1991) IL-1-converting enzyme requires aspartic acid residues for processing of the IL-1β precursor at two distinct sites and does not cleave 31 kDa IL-1α. J. Immunol. 147: 2964-2969
9. Sleath PR, Hendrickson RC, Kronheim SR, March CJ and Black RA (1990) Substrate specificity of the protease that processes human interleukin-1β. J. Biol. Chem. 265: 14526-14528
10. Thornberry NA, Bull HG, Calaycay JR, Chapman KT, Howard AD, Kostura MJ, Miller DK, Molineaux SM, Weidner JR, Aunins J, Elliston KO, Ayala JM, Casano FJ, Chin J, Ding GJ-F, Egger LA, Gaffney EP, Limjuco G, Palyha OC, Raju SM, Rolando AM, Salley JP, Yamin T-T, Lee TD, Shively JE, MacCoss M, Mumford RA, Schmidt JA and Tocci MJ (1992) A novel heterodimeric cysteine protease is required for interleukin-1β processing in monocytes. Nature 356: 768-774
11. Cerretti DP, Kozlosky CJ, Mosley B, Nelson N, Van Ness K, Greenstreet TA, March CJ, Kronheim SR, Druck T, Cannizzaro LA, Huebner K and Black RA (1992) Molecular cloning of the interleukin-1β converting enzyme. Science 256: 97-100
12. Nicholson DW, Ali A, Thornberry NA, Vaillancourt JP, Ding CK, Gallant MG, Y, Griffin PR, Labelle M, Lazebnik YA, Munday NA, Raju AM, Smulson ME, Yamin T-T, Yu VL and Miller DK (1995) Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376: 37-43
13. Rotonda J, Nicholson DW, Fazil KM, Gallant M, Gareau Y, Labelle M, Peterson EP, Rasper DM, Ruel R, Vaillancourt JP, Thornberry NA and Becker JW (1996) The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. Nature Structural Biology 3: 619-625
14. 2 homodimer. Cell 78: 343-352
15. Wilson KP, Black JF, Thomson JA, Kim EE, Griffith JP, Navia MA, Murcko MA, Chambers SP, Aldape RA, Raybuck SA and Livingston DJ (1994) Structure and mechanism of interleukin-1β converting enzyme. Nature 370: 270-275
16. Han Z, Hendrickson EA, Bremner TA and Wyche JH (1997) A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro. J. Biol. Chem. 272: 13432-13436
17. Yamin TT, Ayala JM and Miller DK (1996) Activation of the native 48-kDa precursor form of interleukin-1 converting enzyme. J. Biol. Chem. 271: 13273-13282
18. Stennicke HR and Salvesen GS (1997) Biochemical Characteristics of Caspases-3, -6, -7, and -8. J. Biol. Chem. 272: 25719-25723
19. Chen W, Raybuck SA, Fulghum JR, Petrillo RA, Margolin N and Chambers SP (1997) Expression and purification of human interleukin-1β converting enzyme from Trichopusia ni insect cells using a baculovirus expression system. Protein Expression and Purification 9: 69-75
20. Malinowski JJ, Grasberger BL, Trakshel G, Huston EE, Helaszek CT, Smallwood AM, Ator MA, Banks TM, Brake PG, Ciccarelli RB, Jones BN, Koehn JA, Kratz D, Lundberg N, Stams T, Rubin B, Alexander RS and Stevis PE (1995) Production, purification, and crystallization of human interleukin-1β converting enzyme derived from an Escherichia coli expression systems. Protein Science 4: 2149-2155
21. Sun J, Bottomley SP, Kumar S and Bird PI (1997) Recombinant caspase-3 expressed in Pichia pastoris is fully activated and kinetically indistinguishable from the native enzyme. BBRC 238: 920-924
22. Bradford M (1976) Anal. Biochem. 72: 248
23. Ellis KJ and Morrison JF (1982) Buffers of constant ionic strength for studying pH-dependent processes. Methods in Enzymology 87: 405-426
24. Thornberry NA (1994) Interleukin-1β converting enzyme. Methods in Enzymology 244: 615-631
25. Casciola-Rosen LA, Nicholson DW, Chong T, Rowan KR, Thornberry NA, Miller DK and Rosen A (1996) Apopain/CPP32 cleaves proteins that are essential for cellular repair: A fundamental principle of apoptotic death. J. Exp. Med. 183: 1957-1964
26. Talanian RV, Quinlan C, Trautz S, Hackett MC, Mankovich JA, Banach D, Ghayur T, Brady KD and Wong WW (1997) Substrate specific ties of caspase family proteases. J. Biol. Chem. 272: 9677-9682
27. Rano TA, Timkey T, Peterson EP, Rotonda J, Nicholson DW, Becker JW, Chapman KT and Thornberry NA (1997) A combinatorial approach for determining protease specificities: application to interleukin-1β converting enzyme (ICE). Chemistry and Biology 4: 149-155
28. Garcia-Calvo M, Peterson EP, Leiting B, Ruel R, Nicholson DW and Thornberry NA (1998) Inhibition of human caspases by peptide-based and rnacromolecular inhibitors. J. Biol. Chem. 273: 32608-32613