Entamoeba histolytica lacks trypanothione metabolism

Molecular and Biochemical Parasitology

Volumn 103, Issue 1, 1999, Pages 61-69

  Ariyanayagam, Mark R ^a   Fairlamb, Alan H ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Entamoeba histolytica; Evolution; Glutathione; Mitochondrion; Thiols; Trypanothione

Indexed keywords

GLUTATHIONE; GLUTATHIONE REDUCTASE; TRYPANOTHIONE;

ARTICLE; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVITY; EPIMASTIGOTE; GLUTATHIONE METABOLISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; LEISHMANIA DONOVANI; METABOLITE; PRIORITY JOURNAL; SPECIES DIFFERENCE; TRYPANOSOMA CRUZI;

ANIMALS; ENTAMOEBA HISTOLYTICA; GLUTATHIONE; LEISHMANIA DONOVANI; NADH, NADPH OXIDOREDUCTASES; SPECIES SPECIFICITY; SPERMIDINE; SULFHYDRYL COMPOUNDS; TRYPANOSOMA CRUZI;

ENTAMOEBA HISTOLYTICA; LEISHMANIA DONOVANI; TRYPANOSOMA; TRYPANOSOMA CRUZI; TRYPANOSOMATIDAE;

EID: 0032878178     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(99)00118-8     Document Type: Article

References (34)

1. Meister A., Anderson M.E. Glutathione. Annu. Rev. Biochem. 52:1983;711-760.
2. Fairlamb A.H. Novel biochemical pathways in parasitic protozoa. Parasitology. 99S:1989;93-112.
3. Fahey R.C., Newton G.L., Arrick B., Overdank-Bogart T., Aley S.B. Entamoeba histolytica: a eukaryote without glutathione metabolism. Science. 224:1984;70-72.
4. Brown D.M., Upcroft J.A., Edwards M.R., Upcroft P. Anaerobic bacterial metabolism in the ancient eukaryote Giardia duodenalis. Int. J. Parasitol. 28:1998;149-164.
5. McLaughlin J., Aley S. The biochemistry and functional morphology of the Entamoeba. J. Protozool. 32:1985;221-240.
6. Brown D.M., Upcroft J.A., Upcroft P. Cysteine is the major low-molecular weight thiol in Giardia duodenalis. Mol. Biochem. Parasitol. 61:1993;155-158.
7. Ondarza R.N., Tamayo E.M., Hurtado G., Hernandez E., Iturbe A. Isolation and purification of glutathionyl-spermidine and trypanothione from Entamoeba histolytica. Arch. Med. Res. 28:1997;S73-S75.
8. Fairlamb A.H., Blackburn P., Ulrich P., Chait B.T., Cerami A. Trypanothione: a novel bis(glutathionyl)spermidine cofactor for glutathione reductase in trypanosomatids. Science. 227:1985;1485-1487.
9. Fairlamb A.H., Cerami A. Metabolism and functions of trypanothione in the Kinetoplastida. Annu. Rev. Microbiol. 46:1992;695-729.
10. Tovar J., Wilkinson S., Mottram J.C., Fairlamb A.H. Evidence that trypanothione reductase is an essential enzyme in Leishmania by targeted replacement of the tryA gene locus. Mol. Microbiol. 29:1998;653-660.
11. Tovar J., Cunningham M.L., Smith A.C., Croft S.L., Fairlamb A.H. Down-regulation of Leishmania donovani trypanothione reductase by heterologous expression of a trans-dominant mutant homologue: effect on parasite intracellular survival. Proc. Natl. Acad. Sci. USA. 95:1998;5311-5316.
12. Dumas C., Ouellette M., Tovar J., Cunningham M.L., Fairlamb A.H., Tamar S., Olivier M., Papadopoulou B. Disruption of the trypanothione reductase gene of Leishmania decreases its ability to survive oxidative stress in macrophages. EMBO J. 16:1997;2590-2598.
13. Diamond L.S., Clark C.G., Cunnick C.C. YI-S, a casein-free medium for axenic cultivation of Entamoeba histolytica, related Entamoeba, Giardia intestinalis and Trichomonas vaginalis. J. Euk. Microbiol. 42:1995;277-278.
14. Pereira M.E., Hoff R. Heterogeneous distribution of neuraminidase activity in strains and clones of Trypanosoma cruzi and its possible association with parasite myotropism. Mol. Biochem. Parasitol. 20:1986;183-189.
15. Cunningham M.L., Fairlamb A.H. Trypanothione reductase from Leishmania donovani - purification, characterisation and inhibition by trivalent antimonials. Eur. J. Biochem. 230:1995;460-468.
16. Lo H.-S., Reeves R.E. Purification and properties of NADPH:flavin oxidoreductase from Entamoeba histolytica. Mol. Biochem. Parasitol. 2:1980;23-30.
17. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
18. Shim H., Fairlamb A.H. Levels of polyamines, glutathione and glutathione-spermidine conjugates during growth of the insect trypanosomatid Crithidia fasciculata. J. Gen. Microbiol. 134:1988;807-817.
19. Fairlamb A.H., Henderson G.B., Bacchi C.J., Cerami A. In vivo effects of difluoromethylornithine on trypanothione and polyamine levels in bloodstream forms of Trypanosoma brucei. Mol. Biochem. Parasitol. 24:1987;185-191.
20. Borges A., Cunningham M.L., Tovar J., Fairlamb A.H. Site-directed mutagenesis of the redox-active cysteines of Trypanosoma cruzi trypanothione reductase. Eur. J. Biochem. 228:1995;745-752.
21. 9-bis(glutathionyl)aminopropylcadaverine (homotrypanothione) in Trypanosoma cruzi. Eur. J. Biochem. 226:1994;1019-1027.
22. Newton G.L., Fahey R.C. Determination of biothiols by bromobimane labeling and high-performance liquid chromatography. Methods Enzymol. 251:1995;148-166.
23. Newton G.L., Fahey R.C. Purification of thiols from biological samples. Methods Enzymol. 143:1987;96-101.
24. Fahey R.C., Sundquist A.R. Evolution of glutathione metabolism. Adv. Enzymol. Relat. Areas Mol. Biol. 64:1991;1-53.
25. Margulis L., Chapman M.J. Endosymbioses: cyclical and permanent in evolution. Trends Microbiol. 6:1998;342-345.
26. Margulis L. Archaeal-eubacterial mergers in the origin of Eukarya: phylogenetic classification of life. Proc. Natl. Acad. Sci. USA. 93:1996;1071-1076.
27. Bakker Grunwald T., Wostmann C. Entamoeba histolytica as a model for the primitive eukaryotic cell. Parasitol. Today. 9:1993;27-31.
28. Clark C.G., Roger A.J. Direct evidence for secondary loss of mitochondria in Entamoeba histolytica. Proc. Natl. Acad. Sci. USA. 92:1995;6518-6521.
29. Roger A.J., Svard S.G., Tovar J., Clark C.G., Smith M.W., Gillin F.D., Sogin M.L. A mitochondrial-like chaperonin 60 gene in Giardia lamblia: evidence that diplomonads once harbored an endosymbiont related to the progenitor of mitochondria. Proc. Natl. Acad. Sci. USA. 95:1998;229-234.
30. Gray M.W., Burger G., Lang B.F. Mitochondrial evolution. Science. 283:1999;1476-1481.
31. Bruchhaus I., Richter S., Tannich E. Recombinant expression and biochemical characterization of an NADPH:flavin oxidoreductase from Entamoeba histolytica. Biochem. J. 330:1998;1217-1221.
32. Bruchhaus I., Richter S., Tannich E. Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica. Biochem. J. 326:1997;785-789.
33. Gutteridge W.E., Coombs G.H. Catabolism and the generation of energy. I. Stages parasitic in vertebrates. Gutteridge W.E., Coombs G.H. Biochemistry of Parasitic Protozoa. 1977;32-58 Macmillan, London.
34. Zilberstein D., Dwyer D.M. Glucose transport in Leishmania donovani promastigotes. Mol. Biochem. Parasitol. 12:1984;327-336.