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Volumn 121, Issue 39, 1999, Pages 9221-9222

Evidence of a molecular tunnel connecting the active sites for Co2 reduction and acetyl-CoA synthesis in acetyl-CoA synthase from Clostridium thermoaceticum [2]

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL COENZYME A; ACETYL COENZYME A SYNTHETASE; CARBON DIOXIDE;

EID: 0032864316     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja992120g     Document Type: Letter
Times cited : (85)

References (27)
  • 9
    • 0345664327 scopus 로고    scopus 로고
    • note
    • Reductants were prepared anaerobically. Triquat and Ti(III) citrate were synthesized as described (refs 23 and 24). Viologens (methyl, benzyl, and triquat) were reduced enzymatically in 10 mM MES pH 7.0. Concentrations of the reduced states were determined by titration against a standard potassium ferricyanide solution.
  • 12
    • 0345232276 scopus 로고    scopus 로고
    • note
    • A Wheaton vial containing a solution of MV-reduced hemoglobin was sealed with a rubber septum, flushed with CO for 2 min, incubated 5 min, and then purged with Ar for 2 min. The vial was then opened to the glovebox atmosphere for 5-6 h. Successful (i.e., 100%) conversion of hemoglobin to its CO adduct was confirmed spectrophotometrically.
  • 20
    • 0345232275 scopus 로고    scopus 로고
    • note
    • 2 and CO. Vessels were fabricated from 250 mL Erlenmeyer flasks, each with a 5.0 mL conical-reaction vial fused to the flat bottom and two vacuum stopcocks fused at opposite side walls. A triangular stir bar located at the bottom of the conical vial mixed the solution while a round-tapered stir bar mixed the gas phase.
  • 21
    • 0345232274 scopus 로고    scopus 로고
    • note
    • 18 reversed-phase HPLC.
  • 22
    • 0345664310 scopus 로고    scopus 로고
    • note
    • Midpoint potentials for triquat, MV, and BV were taken as -0.54, -0.44, and -0.35 V vs NHE, respectively (ref 27). Potentials were calculated from the ratio of the reduced to oxidized viologen using the Nernst equation.
  • 26
    • 0344801406 scopus 로고
    • Budavari, S., Ed.; Merck & CO., Inc.: Rahway, NJ
    • Budavari, S. The Merck Index, 11th ed.; Budavari, S., Ed.; Merck & CO., Inc.: Rahway, NJ, 1989; p 533.
    • (1989) The Merck Index, 11th Ed. , pp. 533
    • Budavari, S.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.