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9
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0345664327
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note
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Reductants were prepared anaerobically. Triquat and Ti(III) citrate were synthesized as described (refs 23 and 24). Viologens (methyl, benzyl, and triquat) were reduced enzymatically in 10 mM MES pH 7.0. Concentrations of the reduced states were determined by titration against a standard potassium ferricyanide solution.
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10
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0028021651
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Kumar, M.; Lu, W. P.; Ragsdale, S. W. Biochemistry 1994, 33, 9769-9777.
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Lu, W.P.2
Ragsdale, S.W.3
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12
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0345232276
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note
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A Wheaton vial containing a solution of MV-reduced hemoglobin was sealed with a rubber septum, flushed with CO for 2 min, incubated 5 min, and then purged with Ar for 2 min. The vial was then opened to the glovebox atmosphere for 5-6 h. Successful (i.e., 100%) conversion of hemoglobin to its CO adduct was confirmed spectrophotometrically.
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13
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0025191934
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Lindahl, P. A.; Ragsdale, S. W.; Münck, E. J. Biol. Chem. 1990, 265, 3880-3888.
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0030957783
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Raushel, F.M.4
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15
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0024297340
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Hyde, C. C.; Ahmed, S. A.; Padlan, E. A.; Miles, E. W.; Davies, D. R. J. Biol. Chem. 1988, 263, 17857-17871.
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0342894815
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Krahn, J. M.; Kim, J. H.; Burns, M. R.; Parry, R. J.; Zalkin, H.; Smith, J. L. Biochemistry 1997, 36, 11061-11068.
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18
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0024425298
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20
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0345232275
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note
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2 and CO. Vessels were fabricated from 250 mL Erlenmeyer flasks, each with a 5.0 mL conical-reaction vial fused to the flat bottom and two vacuum stopcocks fused at opposite side walls. A triangular stir bar located at the bottom of the conical vial mixed the solution while a round-tapered stir bar mixed the gas phase.
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-
-
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21
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0345232274
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note
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18 reversed-phase HPLC.
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-
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22
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0345664310
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note
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Midpoint potentials for triquat, MV, and BV were taken as -0.54, -0.44, and -0.35 V vs NHE, respectively (ref 27). Potentials were calculated from the ratio of the reduced to oxidized viologen using the Nernst equation.
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26
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0344801406
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Budavari, S., Ed.; Merck & CO., Inc.: Rahway, NJ
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Budavari, S. The Merck Index, 11th ed.; Budavari, S., Ed.; Merck & CO., Inc.: Rahway, NJ, 1989; p 533.
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