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Volumn 119, Issue 17, 1997, Pages 3959-3970

Methylation of carbon monoxide dehydrogenase from Clostridium thermoaceticum and mechanism of acetyl coenzyme A synthesis

Author keywords

[No Author keywords available]

Indexed keywords

1,10 PHENANTHROLINE; ACETYL COENZYME A; CARBON MONOXIDE DEHYDROGENASE; CYANIDE; CYSTEINE; NICKEL;

EID: 0031006876     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja963597k     Document Type: Article
Times cited : (100)

References (68)
  • 3
    • 0003768271 scopus 로고
    • Chapman & Hall: New York
    • (c) Acetogenesis; Drake, H. L., Ed.; Chapman & Hall: New York, 1994.
    • (1994) Acetogenesis
    • Drake, H.L.1
  • 4
    • 1842394824 scopus 로고    scopus 로고
    • note
    • red, the oxidized and reduced states of the D site.
  • 32
    • 1842321395 scopus 로고    scopus 로고
    • note
    • 21 arose from the Ni of the C-cluster rather than from that of the A-cluster.
  • 48
    • 1842313776 scopus 로고    scopus 로고
    • note
    • 1+ feature in the absorption spectrum of CoFeSP from developing when reductants were added and inhibited methylation of CoFeSP. The possibility that dithionite binds to CoFeSP should be evaluated further.
  • 50
    • 1842358144 scopus 로고    scopus 로고
    • note
    • 3+FeSP in the solution had no bearing as to whether the methyl groups already bound to CODH were functional. Those methyl groups on CODH reacted with CO and CoA to yield acetyl-CoA (or acetate), demonstrating that they must have been functionally bound to CODH. (46) The NiFeC signal intensity of the methylated sample treated with phen, CO, and CoA may have been lower than that of the control sample (which lacked phen) because the phen probably removed some of the labile Ni during the period after the sample was "demethylated" by CO and CoA and before it was frozen.
  • 51
    • 1842319471 scopus 로고    scopus 로고
    • note
    • av = 1.86 signal.
  • 52
    • 1842388021 scopus 로고    scopus 로고
    • note
    • 26 measured 0.2-0.3 labile Ni/αβ, for samples with average NiFeC spin intensities and exchange activities of 0.14 spins/αβ and 0.16 units/mg, respectively. Due to improved bacterial harvesting and enzyme purification methods (harvesting anaerobically and purifying rapidly and immediately after harvesting), our NiFeC spin intensities (0.3-0.5 spins/ αβ) and exchange activities (∼0.4 units/mg) are significantly higher. Since we obtained an average of ∼0.5 methyl groups bound per αβ, the CODH used here may have contained more labile nickel than the lower-activity samples studied previously.
  • 64
    • 1842393918 scopus 로고    scopus 로고
    • note
    • 13CO state and the hyperfine-broadened signal.
  • 68
    • 1842308018 scopus 로고    scopus 로고
    • note
    • red-CO state, proposing that CO binds to the labile Ni in other states is compatible with, though not supported by, that result.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.