Simple, rapid, quantitative, and sensitive detection of telomere repeats in cell lysate by a hybridization protection assay

Clinical Chemistry

Volumn 45, Issue 10, 1999, Pages 1718-1724

  Nakamura, Yasuhiro ^a   Hirose, Minoru ^b   Matsuo, Hajime ^a   Tsuyama, Naohiro ^a   Kamisango, Keiichi ^b   Ide, Toshinori ^a  

^a HIROSHIMA UNIV SCHOOL OF MEDICINE   (Japan)

^b Chugai Diagnostics Science Co Ltd   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ARTICLE; CELL LYSATE; DIAGNOSTIC PROCEDURE; DIAGNOSTIC VALUE; DNA HYBRIDIZATION; FIBROBLAST; HUMAN; HUMAN CELL; QUANTITATIVE ASSAY; SOUTHERN BLOTTING; TELOMERE;

EID: 0032864304     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/45.10.1718     Document Type: Article

References (16)

1. 1. Blackburn EH, Greider CW, eds. Telomeres. Cold Spring Harbor, NY; Cold Spring Harbor Laboratory Press, 1995:396pp.
2. 2. Zakian AV. Structure and function of telomeres. Annu Rev Genet 1989;23:579-604.
3. 3. Harley CB, Futcher AB, Greider CW. Telomeres shorten during ageing of human fibroblasts. Nature 1990;345:458-60.
4. 4. Allsopp RC, Vaziri H, Patterson C, Goldstein S, Younglai EV, et al. Telomere length predicts replicative capacity of human fibroblasts. Proc Natl Acad Sci U S A 1992;89:10114-8.
5. 5. Hastie ND, Dempster M, Dunlop MG, Thompson AM, Green DK, Alishire RC. Telomere reduction in human colorectal carcinoma and with ageing. Nature 1993;346:866-8.
6. 6. Vaziri H, Schachter F, Uchida I, Wei L, Zhu X, Effros R, et al. Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes. Am J Hum Genet 1993;52:661-7.
7. 7. Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu C-P, Morin GB, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 1998;279:349-53.
8. 8. Vaziri H, Benchimol S. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr Biol 1998;8:279-82.
9. 9. Hirose M, Hashimoto JA, Ogura K, Tahara H, Ide T, Yoshimura T. A rapid, useful and quantitative method to measure telomerase activity by hybridization protection assay connected with telomeric repeat amplification protocol. J Cancer Res Clin Oncol 1997;123:337-44.
10. 10. Hirose M, Hashimoto JA, Tahara H, Ide T, Yoshimura T. New method to measure telomerase activity by transcription-mediated amplification and hybridization protection assay. Clin Chem 1998;44:2446-52.
11. 11. Tahara H, Tokutake Y, Maeda S, Kataoka H, Watanabe T, Satoh M, et al. Abnormal telomere dynamics of B-lymphoblastoid cell strains from Werner's syndrome patients transformed by Epstein-Barr virus. Oncogene 1997;15:1911-20.
12. 12. Matsuo M, Kaji K, Utakoji T, Hosoda K. Ploidy of human embryonic fibroblasts during in vitro aging. J Gerontol 1982;37:33-7.
13. 13. Tsuyama N, Miura M, Kitahira M, Ishibashi S, Ide T. SV40 T-antigen is required for maintenance of immortal growth in SV40-transformed human fibroblasts. Cell Struct Funct 1991;16:55-62.
14. 14. Lansdorp PM, Verwoerd NP, van de Rijke FM, Dragowska V, Little M-T, Dirks RW, et al. Heterogeneity in telomere length of human chromosomes. Hum Mol Genet 1996;5:685-91.
15. 15. Norwood D, Dimitrov DS. Sensitive method for measuring telomere length by quantifying telomeric DNA content of whole cells. Biotechniques 1998;25:1040-5.
16. 16. Rufer N, Dragowska W, Thornbury G, Roosnek E, Lansdorp P M. Telomere length dynamics in human lymphocytes subpopulations measured by flow cytometry. Nat Biotechnol 1998;6:743-7.