Development of a direct in situ RT-PCR method using labeled primers to detect cytokine mRNA inside cells

Journal of Immunological Methods

Volumn 227, Issue 1-2, 1999, Pages 149-160

  Gey, Alain ^a   Hamdi, Safouane ^a   Vielh, Philippe ^a   Mehtali, M ^b   Fridman, Wolf H ^a   Tartour, Eric ^a  

^a INSTITUT CURIE   (France)

^b TRANSGENE S A   (France)

Author keywords

Cytokines, Labeled primer; In situ RT PCR; T lymphocyte activation

Indexed keywords

CELL EXTRACT; CYTOKINE; DIGOXIGENIN; DNA; DNA POLYMERASE; FLUORESCEIN; GAMMA INTERFERON; INTERLEUKIN 2; MESSENGER RNA; PRIMER DNA;

ANIMAL CELL; ARTICLE; BIOTINYLATION; CELL STRUCTURE; CELLULAR DISTRIBUTION; CHO CELL; CONTROLLED STUDY; GENE AMPLIFICATION; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; T LYMPHOCYTE; TUMOR ASSOCIATED LEUKOCYTE;

ANIMALS; CHO CELLS; CRICETINAE; CYTOKINES; DIFFUSION; HUMANS; INTERFERON TYPE II; LYMPHOCYTE ACTIVATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; T-LYMPHOCYTES; TUMOR CELLS, CULTURED;

ANIMALIA; THERMUS THERMOPHILUS;

EID: 0032842546     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00078-2     Document Type: Article

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