Evaluation of the effects of DNase treatment on signal specificity in RT-PCR and in situ RT-PCR

BioTechniques

Volumn 25, Issue 4, 1998, Pages 630-638

  Ivarsson, Karin ^a,b   Weijdegård, Birgitta ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEASE;

ARTICLE; DNA DETERMINATION; DNA SYNTHESIS; ENZYME SPECIFICITY; GENE AMPLIFICATION; GENE TARGETING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL DETECTION;

EID: 0031715327     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98254st04     Document Type: Article

References (8)

1. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
2. Huang, Z., M.J. Fasco and L.S. Kaminsky. 1996. Optimization of DNase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR. BioTechniques 20:1012-1020.
3. Martinez, A., M.-J. Miller, K. Quinn, E.J. Unsworth, M. Ebina and F. Cuttitta. 1995. Non-radioactive localization of nucleic acids by direct in situ PCR and in situ RT-PCR in paraffin-embedded sections. J. Histochem. Cytochem. 43:739-747.
4. Menon, R.S., Y.-F. Chang, J. St. Clair and R.G. Ham. 1991. RT-PCR artifacts from processed pseudogenes. PCR Methods Appl. 1:70-71.
5. Nudel, U., R. Zakut, M. Shani, S. Neuman, Z. Levy and D. Yaffe. 1983. The nucleotide sequence of the rat cytoplasmic beta-actin gene. Nucleic Acids Res. 11:1759-1771.
6. Nuovo, G.J. 1997. PCR In Situ Hybridization: Protocols and Applications, 3rd ed., p. 271-334. Lippincott-Raven, Philadelphia.
7. Sallstrom, J.F., I. Zehbe, M. Alemi and E. Wilanden 1993. Pitfalls of in situ polymerase chain reaction (PCR) using direct incorporation of labelled nucleotides. Anticancer Res. 13:1153.
8. Strauss, W.M. 1994. Preparation of genomic DNA from mammalian tissue, p. 2.2.1-2.2.3. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology, John Wiley & Sons, New York.