A novel, rapid in cell RNA amplification technique for the detection of low copy mRNA transcripts

Journal of Clinical Pathology - Molecular Pathology

Volumn 51, Issue 3, 1998, Pages 160-163

  Uhlmann, V ^a   Rolfs, A ^b   Mix, E ^b   Silva, I ^a   Hully, J ^c   Lu, L ^c   Lohman, K ^c   Howells, D ^d   Picton, S ^d   O'Leary, J J ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b UNIVERSITY OF ROSTOCK   (Germany)

^c APPLIED BIOSYSTEMS   (United States)

^d PERKINELMER INC   (United States)

Author keywords

'Permeafix'; Pyruvate dehydrogenase; Recombinant Thermus thermophilus DNA polymerase; Reverse transcription polymerase chain reaction in situ hybridization

Indexed keywords

COMPLEMENTARY DNA; DNA POLYMERASE; MESSENGER RNA; RECOMBINANT ENZYME;

ARTICLE; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; LYMPHOCYTE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THERMUS THERMOPHILUS;

EID: 14444271597     PISSN: 13668714     EISSN: None     Source Type: Journal    
DOI: 10.1136/mp.51.3.160     Document Type: Article

References (18)

1. Koike K, Urata Y, Koike M. Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene. Proc Natl Acad Sci USA 1990;87:5594-7.
2. Teo IA, Shaunak S. Polymerase chain reaction in situ: an appraisal of an emerging technique. Histochem J 1995;27: 647-59.
3. Pizzolo G, Vincenzi C, Nadali G, et al. Detection of membrane and intracellular antigens by flow cytometry following ORTHO Permeafix fixation. Leukemia 1994;8: 672-6.
4. Francis C, Connelly MC. Rapid single-step method for flow cytometric detection of surface and intracellular antigens using whole blood. Cytometry 1996;25:58-70.
5. Groeneveld K, te Marvelde JG, van de Beemd MW, et al. Flow cytometric detection of intracellular antigens for immunophenotyping of normal and malignant leukocytes. Leukemia 1996;10:1383-9.
6. Van Zaanen HC, Vet RJ, de Jong CM, et al. A simple and sensitive method for determining plasma cell isotype and monoclonaliry in bone marrow using flow cytometry. Br J Haematol 1995;91:55-9.
7. Patel VG, Shum-Siu A, Heniford BW, et al. Detection of epidermal growth factor receptor mRNA in tissue sections from biopsy specimens using in situ polymerase chain reaction. Am J Pathol 1994;144:7-14.
8. Chen RH, Fuggle SV. In situ cDNA polymerase chain reaction: a novel technique for detecting mRNA expression. Am J Pathol 1993;143:1527-34.
9. Embleton M, Gorochov G, Jones P, et al. In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within cells. Nucleic Acids Res 1992;20:3831-7.
10. Kelleher MB, Galutira D, Duggan TD, et al. Progressive multifocal leukoencephalopathy in a patient with Alzheimer's disease. Diagn Mol Pathol 1994;3:105-13.
11. Nuovo GJ, Lidonnici K, MacConnell P, et al. Intracellular localization of PCR amplified hepatitis C cDNA. Am J Surg Pathol 1993;139:1239-44.
12. Staecker H, Cammer M, Rubinstein R, et al. A procedure for RT-PCR amplification of mRNAs in histological specimens. Biotechniques 1994;16:76-80.
13. Peters J, Krams M, Wacker HH, et al. Detection of rare RNA sequences by single-enzyme in situ reverse transcription-polymerase chain reaction. High resolution analyses of interleukin-6 mRNA in paraffin sections of lymph nodes. Am J Pathol 1997;150:469-76.
14. Heniford BW, Shum-Siu A, Leonberger M, et al. Variation in cellular EGF receptor expression demonstrated by in situ reverse transcriptase polymerase chain reaction. Nucleic Acids Res 1993;21:3159-66.
15. Patterson BK, Till M, Otto P, et al. Detection of HIV-1 DNA and messenger RNA in individual cells by PCR-driven in-situ hybridization and flow cytometry. Science 1993;360:976-9.
16. Nuovo GJ. In situ hybridization: protocols and applications. New York: Raven Press, 1992.
17. Myers TW, Sigua CL. Amplification of RNA: high temperature reverse transcription and DNA amplification with Thermus thermophilus DNA polymerase. In: Innis MA, Gelfand DH, Sninsky JJ, eds. PCR strategies. San Diego, CA: Academic Press Inc, 1994:58-68.
18. Myers TW, Gelfand DH. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 1991;30:7661-6.