The ferric uptake regulator (Fur) homologue of Helicobacter pylori: Functional analysis of the coding gene and controlled production of the recombinant protein in Escherichia coli

Medical Microbiology and Immunology

Volumn 188, Issue 1, 1999, Pages 31-40

  Bereswill, Stefan ^a   Lichte, Flavia ^a   Greiner, Stefan ^a   Waidner, Babara ^a   Fassbinder, Frank ^a   Kist, Manfred ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Fur regulon; Helicobacter pylori; Iron uptake; Iron dependent regulation

Indexed keywords

BACTERIAL PROTEIN; FERRIC ION; FERRIC UPTAKE REGULATOR PROTEIN; RECOMBINANT PROTEIN; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; CROSS REACTION; DNA SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE INACTIVATION; HELICOBACTER PYLORI; IMMUNOBLOTTING; IRON TRANSPORT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA INTERACTION; PROTEIN SYNTHESIS; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BETA-GALACTOSIDASE; BLOTTING, SOUTHERN; DRUG RESISTANCE, MICROBIAL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HELICOBACTER PYLORI; IRON; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; REPRESSOR PROTEINS; SEQUENCE ANALYSIS, DNA;

EID: 0032823648     PISSN: 03008584     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004300050102     Document Type: Article

References (47)

1. Alm RA, Ling LS, Moir DT, et al (1999) Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397:176-180
2. Asaka M, Kimura T, Kato M, Kudo M, Miki K, Ogoshi K, Kato T, Tatsuga M, Graham DY (1994) Possible role of Helicobacter infection in early gastric cancer development. Cancer 73:2691-2694
3. Atherton JC, Cao P, Peek RM, Tummuru MKR, Blaser JM, Cover TL (1995) Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. J Biol Chem 270:17771-17777
4. Barton HA, Johnson Z, Cox CD, Vasil AI, Vasil ML (1996) Ferric uptake regulator mutants of Pseudomonas aeruginosa with distinct alterations in the iron-dependent repression of exotoxin A and siderophores in aerobic and microaerobic environments. Mol Microbiol 21:1001-1017
5. Bereswill S, Fassbinder F, Voelzing C, Haas R, Reuter K, Ficner R, Kist M (1997) Cloning and functional characterization of the genes encoding 3-dehydroquinate synthase (aroB) and tRNA-guanine transglycosylase (tgt) from Helicobacter pylori. Med Microbiol Immunol 186:125-134
6. Bereswill S, Fassbinder F, Voelzing C, Covacci A, Haas R, Kist M (1998) Hemolytic properties and riboflavin synthesis of Helicobacter pylori: Cloning and functional characterization of the ribA gene encoding GTP-cyclohydrolase II that confers hemolytic activity to Escherichia coli. Med Microbiol Immunol 186:177-187
7. Bereswill S, Lichte F, Vey T, Fassbinder F, Kist M (1998) Cloning and characterization of the fur gene from Helicobacter pylori. FEMS Microbiol Lett 159:193-200
8. Bereswill S, Waidner U, Odenbreit S, Lichte F, Fassbinder F, Bode G, Kist M (1998) Structural, functional and mutational analysis of the pfr gene encoding a ferritin from Helicobacter pylori. Microbiology 144:2505-2516
9. Bezkorovainy A (1987) Iron proteins. In: Iron and Infection: Molecular, Physiological and Clinical Aspects. Wiley, Chichester, pp 51-55
10. Blum AL (1996) Helicobacter pylori and peptic ulcer disease. Scand J Gastroenterol 214:24-27
11. Bodger K, Crabtree JE (1998) Helicobacter pylori and gastric inflammation. Br Med Bull 54:139-150
12. Brickman, TJ, Armstrong SK (1995) Bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated Bordetella bronchiseptica mutants. J Bacteriol 177:268-270
13. Bukanov NO, Berg DE (1994) Ordered cosmid library and high resolution physical genetic map of Helicobacter pylori strain NCTC 11638. Mol Microbiol 11:509-523
14. Censini S, Lange C, Xiang Z, Crabtree JE, Ghiara P, Borodovsky M, Rappuoli R, Covacci A (1996) Cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc Natl Acad Sci USA 93:14648-14653
15. Dhaenens L, Szczebara F, Husson MO (1997) Identification, characterization, and immunogenicity of the lactoferrin-binding protein from Helicobacter pylori. Infect Immun 65:514-518
16. Dunn BE, Cohen H, Blaser MJ (1997) Helicobacter pylori. Clin Microbiol Rev 10:720-741
17. Earhart CF (1996) Uptake and metabolism of iron and molybdenium. In: Neidhardt FC (ed) Escherichia coli and Salmonella typhimurium, Cellular and Molecular Biology, 2nd Edn, vol I. American Society for Microbiology Press, Washington, DC, pp 1075-1090
18. Hantke K (1987) Selection procedure for deregulated iron transport mutants (fur) in Escherichia coli K12: Fur not only affects iron metabolism. Mol Gen Genet 210:135-139
19. Harrison PM, Arioso P (1996) The ferritins: Molecular properties, iron storage function and cellular regulation. Biochim Biophys Acta 1275:161-203
20. Hassett DJ, Sokol PA, Howell ML, Ma JF, Schweizer HT, Ochsner U, Vasil ML (1996) Ferric uptake regulator (Fur) mutants of Pseudomonas aeruginosa demonstrate defective siderophore-mediated iron uptake, altered aerobic growth, and decreased superoxide dismutase and catalase activities. J Bacteriol 178:3996-4003
21. Husson MO, Legrand D, Spik G, Leclerc H (1993) Iron acquisition by Helicobacter pylori: Importance of human lactoferrin. Infect Immun 61:2694-2697
22. Litwin CM, Calderwood SB (1993) Role of iron in regulation of virulence genes. Clin Microbiol Rev 6:137-149
23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-275
24. Marshall DG, Dundon WG, Beesley SM, Smyth CJ (1998) Helicobacter pylori-a conundrum of genetic diversity. Microbiology 144:2925-2939
25. Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor
26. Nakao K, Imoto I, Gabazza EC, Yamauchi K, Yamazaki N, Taguchi Y, Shibata T, Takaji S, Ikemura N, Misaki M (1997) Gastric juice levels of lactoferrin and Helicobacter pylori infection. Scand J Gastroenterol 32:530-534
27. Nakao K, Imoto I, Ikemura N, Shibata T, Takaji S, Taguchi Y, Misaki M, Yamauchi K, Yamazaki N (1997) Relation of lactoferrin levels in gastric mucosa with Helicobacter pylori infection and with the degree of gastric inflammation. Am J Gastroenterol 92:1005-1011
28. Prince RW, Cox CD, Vasil ML (1993) Coordinate regulation of siderophore and exotoxin A production: Molecular cloning and sequencing of the Pseudomonas aeruginosa fur gene. J Bacteriol 175:2589-2598
29. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning a Laboratory Manual, 2nd Edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
30. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-termination inhibitors. Proc Natl Acad Sci USA 74:5463-5467
31. Schäffer S, Hantke K, Braun V (1985) Nucleotide sequence of the iron regulatory gene fur. Mol Gen Genet 200:110-113
32. Spiegelhalder C, Gerstenecker B, Kersten A, Schiltz E, Kist M (1993) Purification of Helicobacter pylori superoxide dismutase and cloning and sequencing of the gene. Infect Immun 61:5315-5325
33. Stojiljkovic I, Hantke K (1995) Functional domains of the Escherichia coli ferric uptake regulator protein. Mol Gen Genet 247:199-205
34. Stojiljkovic I, Bäumler AJ, Hantke K (1994) Fur regulon in gram-negative bacteria: Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay. J Mol Biol 236:531-545
35. Strobel S, Bereswill S, Allgaier P, Balig P, Sonntag H-G, Kist M (1998) Identification and analysis of a new vacA genotype variant of Helicobacter pylori in different patient groups in Germany. J Clin Microbiol 36:1285-1289
36. Szczebara F, Dhaenens S, Armand S, Husson MO (1998) Cloning and characterization of a fur homologue from Helicobacter mustelae and Helicobacter felis. Gut 43 [Suppl 2]:A116
37. Taylor DE (1996) Diversity among Helicobacter pylori isolates. J Clin Microbiol 34:1041
38. Thomas CE, Sparling PF (1996) Isolation and analysis of a fur mutant of Neisseria gonorrhoeae. J Bacteriol 178:4224-4232
39. Tomb JF, White O, Kerlavage AR, et al. (1997) The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388:539-547
40. Velayudhan J, Hughes NJ, Andrews SC, Clayton CL, Kelly DJ (1997) Iron acquisition in Helicobacter pylori: Characterization of tbpA, fecA, and exbB homologues. Gut 41 [Suppl 1]:A109
41. Velayudhan J, Hughes NJ, Andrews SC, Clayton CL, Kelly DJ (1998) Cloning, mutagenesis and functional characterization of Helicobacter pylori iron assimilation systems. Proceedings of the 3rd International Workshop on Host Response in Helicobacter pylori Infections, 1998, Helsingore. European H. pylori Study Group (EHPSG), abstr, no. E7
42. van Vliet AHM, Wooldridge KG, Ketley JM (1998) Iron-responsive gene regulation in a Campylobacter jejuni fur mutant. J Bacteriol 180:5291-5298
43. Wertheimer AM, Tolmasky ME, Actis LA, Crosa JH (1994) Structural and functional analyses of mutant Fur proteins with impaired regulatory function. J Bacteriol 176:5116-5122
44. Wooldridge KG, Williams PH, Ketley JM (1994) Iron-responsive genetic regulation in Campylobacter jejuni: Cloning and characterization of a fur homolog. J Bacteriol 176:5852-5856
45. Worst DJ, Otto BR, de Graaff J (1995) Iron-repressible outer membrane proteins of Helicobacter pylori involved in heme uptake. Infect Immun 63:4161-4165
46. Worst DJ, Sparrius M, Kuipers EJ, Kusters JG, de Graaff J (1996) Human serum antibody response against iron-repressible outer membrane proteins of Helicobacter pylori. FEMS Microbiol Lett 144:29-32
47. Zimmermann L, Angerer A, Braun V (1989) Mechanistically novel iron-III transport system in Serratia marcescens. J Bacteriol 171:238-243