Generation and characterization of a stable soluble guanylate cyclase- overexpressing CHO cell line

Nitric Oxide - Biology and Chemistry

Volumn 3, Issue 1, 1999, Pages 55-66

  Becker, Eva Maria ^a   Wunder, Frank ^a   Kast, Raimund ^a   Robyr, Chantal ^a   Hoenicka, Markus ^b   Gerzer, Rupert ^b   Schröder, Henning ^c   Stasch, Johannes Peter ^a  

^a BAYER AG   (Germany)

^b GERMAN AEROSPACE CENTER DLR   (Germany)

^c MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

CGMP; CHO; Nitric oxide; Soluble guanylate cyclase; YC 1

Indexed keywords

1 BENZYL 3 (5 HYDROXYMETHYL 2 FURYL)INDAZOLE; 1H 1,2,4 OXADIAZOLO[4,3 A]QUINOXALIN 1 ONE; CYCLIC GMP; GUANYLATE CYCLASE; GUANYLATE CYCLASE ACTIVATOR; GUANYLATE CYCLASE INHIBITOR; NITRIC OXIDE; NITRIC OXIDE DONOR; NITROPRUSSIDE SODIUM; POLYCLONAL ANTIBODY;

ANIMAL CELL; CHO CELL; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME SUBUNIT; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALIA;

EID: 0032812465     PISSN: 10898603     EISSN: None     Source Type: Journal    
DOI: 10.1006/niox.1999.0207     Document Type: Conference Paper

References (40)

1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
2. Buechler W. A., Nakane M., Murad F. Expression of soluble guanylate cyclase activity requires both enzyme subunits. Biochem. Biophys. Res. Commun. 174:1991;351-357.
3. Buechler W. A., Singh S., Aktas J., Mueller S., Murad F., Gerzer R. High-level expression of biologically active soluble guanylate cyclase using the baculovirus system is strongly heme-dependent. Adv. Pharmacol. 34:1995;293-303.
4. 2+. Circ. Res. 82:1998;686-695.
5. Foersterman U., Mülsch A., Boehme E., Busse R. Stimulation of soluble guanylate cyclase by an acetylcholine induced endothelium derived factor from rabbit and canine arteries. Circ. Res. 58:1986;531-538.
6. Friebe A., Schultz G., Koesling D. Sensitizing soluble guanylate cyclase to become a highly CO-sensitive enzyme. EMBO J. 15:1996;6863-6868.
7. Friebe A., Koesling D. Mechanism of YC-1 induced activation of soluble guanylyl cyclase. Mol. Pharmacol. 53:1998;123-127.
8. Furchgott R. F., Zawadzki J. V. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 288:1980;373-376.
9. Garthwaite J., Southam E., Boulton C. L., Nielsen E. B., Schmidt K., Mayer B. Potent and selective inhibition of nitric oxide-sensitive guanylyl cyclase by 1H[1,2,4] oxadiazolo[4,3-a]quinoxalin-1-one. Mol. Pharmacol. 48:1995;184-188.
10. Gerzer R., Boehme E., Hofmann F., Schultz G. Soluble guanylate cyclase purified from bovine lung contains heme and copper. FEBS Lett. 132:1981;71-74.
11. Harteneck C., Koesling D., Soeling A., Schultz G., Boehme E. Expression of soluble guanylate cyclase - Catalytic activity requires two enzyme subunits. FEBS Lett. 272:1990;221-223.
12. Harteneck C., Wedel B., Koesling D., Malkewitz J., Boehme E., Schultz G. Molecular cloning and expression of a new α-subunit of soluble guanylyl cyclase. FEBS Lett. 292:1991;217-222.
13. Hoenicka M., Becker E. M., Apeler H., Sirichoke T., Schröder H., Gerzer R., Stasch J.-P. Purified soluble guanylyl cyclase expressed in a baculovirus/Sf9 system: Stimulation by YC-1, NO and CO. J. Mol. Med. 77:1999;14-23.
14. Ignarro L. J., Dengan J. N., Barricos W. H., Kadowitz P. J., Wolin M. S. Activation of purified guanylate cyclase by nitric oxide requires heme: Comparison of heme-deficient, heme-reconstituted and heme-containing forms of soluble enzyme from bovine lung. Biochim. Biophys. Acta. 718:1982;49-59.
15. Ishii K., Sheng H., Warner T. D., Foerstermann U., Murad F. A simple and sensitive bioassay method for detection of EDRF with RFL-6 rat lung fibroblasts. Am. J. Physiol. 261:1991;H598-H603.
16. Ko F. N., Wu C.-C., Kuo S.-C., Lee F.-Y., Teng C.-M. YC-1, a novel activator of platelet guanylate cyclase. Blood. 84:1994;4226-4233.
17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
18. Mülsch A., Bauersachs J., Schaefer A., Stasch J.-P., Kast R., Busse R. Effect of YC-1, an NO-independent, superoxide-sensitive stimulator of soluble guanylyl cyclase, on smooth muscle responsiveness to nitrovasodilators. Br. J. Pharmacol. 120:1997;681-689.
19. Myers P. R., Minor R. L. Jr., Guerra R. Jr., Bates J. N., Harrison D. G. Vasorelaxant properties of the endothelium derived relaxing factor more closely resembleS. Nature. 345:1990;161-163.
20. Nakane M., Arai K., Saheki S., Kuno T., Buechler W., Murad F. Molecular cloning and expression of cDNAs coding for soluble guanylate cyclase from rat lung. J. Biol. Chem. 265:1990;16841-16845.
21. Olesen S.-P., Drejer J., Axelsson O., Moldt P., Bang L., Nielsen-Kudsk J. E., Busse R., Muelsch A. Characterization of NS 2028 as a specific inhibitor of soluble guanylyl cyclase. Br. J. Pharmacol. 123:1998;299-309.
22. Olson L. J., Knych E. T. Jr., Herzig T. C., Drewett J. G. Selective guanylyl cyclase inhibitor reverses nitric oxide-induced vasorelaxation. Hypertension. 29:1997;254-261.
23. Schini-Kerth V. B., Vanhoutte P. M. Nitric oxide synthases in vascular cells. Exp. Phys. 80:1995;885-905.
24. Schröder H., Leitmann D. C., Bennett B. M., Waldmann S. A., Murad F. Glyceryl trinitrate-induced desensitization of guanylate cyclase in cultured rat lung fibriblasts. J. Pharmacol. Exp. Ther. 245:1988;413-418.
25. Stone J. R., Marletta M. A. The ferrous heme of sGC: Formation of hexacoordinate complexes with CO and nitrosomethane. Biochemistry. 34:1995;16397-16403.
26. Stone J. R., Marletta M. A. Synergistic activation of soluble guanylate cyclase by YC-1 and carbon monoxide: Implications for the role of cleavage of iron-histidine bond during activation by nitric oxide. Chem. Biol. 5:1998;255-261.
27. Teng C. M., Wu C. C., Ko F.-N., Lee F. Y., Kuo S. C. YC-1, a nitric oxide-independent activator of soluble guanylate cyclase, inhibits platelet-rich thrombosis in mice. Eur. J. Pharmacol. 320:1997;161-166.
28. Towbin H., Staehlin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. 76:1979;4350-4354.
29. Verma A. D., Hirsch J., Glatt C. E., Ronett G. V., Snyder S. H. Carbon monoxide: A putative neuronal messenger. Science. 259:1993;281-284.
30. Wedel B. J., Garbers D. L. New insights on the functions of the guanylyl cyclase receptors. FEBS Lett. 410:1997;29-33.
31. Wegener J. W., Gath I., Foerstermann U., Nawrath H. Activation of soluble guanylyl cyclase by YC-1 in aortic smooth muscle but not in ventricular myocardium from rat. Br. J. Pharmacol. 122:1997;1523-1529.
32. Wegener J. W., Nawrath H. Differential effects of isoliquiritigenin and YC-1 in rat aortic smooth muscle. Eur. J. Pharmacol. 323:1997;89-91.
33. Wohlfart P., Schindler U., Ruetten H., Martorana P., Rudolphi K., Strobel H., Schoenafinger K., Malinski T., Wiemer G. Release of nitric oxide from endothelial cells induced by YC-1, an activator of soluble guanylyl cyclase. Nitric Oxide. 2:1998;130.
34. Wong S. K.-F., Garbers D. L. Receptor guanylyl cyclases. J. Clin. Invest. 90:1992;299-305.
35. Wu C. C., Ko F.-N., Kuo S.-C., Lee F. Y., Teng C. M. YC-1 inhibited human platelet aggregation through NO-independent activation of soluble guanylate cyclase. Br. J. Pharmacol. 116:1995;1973-1978.
36. Wu C. C., Ko F. N., Teng C. M. Inhibition of platelet adhesion to collagen by cGMP-elevating agents. Biochem. Biophys. Res. Commun. 231:1997;412-416.
37. Yoshina S., Kuo S. C. Studies on heterocyclic compounds. XXXV. Synthesis of furo[3,2-c]pyrazole derivatives. (2) Electrophilic substitution of 1,3-diphenylfuro[3,2-c]pyrazole. Yakugaku Zasshi. 98:1978;204-208.
38. Yu S. M., Cheng Z. J., Guh J. H., Lee F. Y., Kuo S. C. Mechanism of anti-proliferation caused by YC-1, an indazole derivative, in cultured rat A10 vascular smooth-muscle cells. Biochem. J. 306:1995;787-792.
39. Zhao Y., Marletta M. A. Localization of the heme binding region in soluble guanylate cyclase. Biochemistry. 36:1997;15959-15964.
40. Zhao Y., Schelvis J. P. M., Babcock G. T., Marletta M. A. Identification of Histidine 105 in the β1 subunit of soluble guanylate cyclase as the heme proximal ligand. Biochemistry. 37:1998;4502-4509.