Characterization of the isoforms of PIXY321, a granulocyte-macrophage-colony stimulating factor-interleukin-3 fusion protein, separated by preparative isoelectric focusing on immobilized pH gradients

Journal of Chromatography A

Volumn 846, Issue 1-2, 1999, Pages 143-156

  Balland, Alain ^a   Mahan Boyce, Julia A ^a   Krasts, Dace A ^a   Daniels, Mark ^b   Wang, Wesley ^a   Gombotz, Wayne R ^a  

^a AMGEN INC   (United States)

^b Amersham Biosciences   (United States)

Author keywords

Glycosylation; Mannose; PIXY321; Preparative electrophoresis; Proteins

Indexed keywords

AMINO ACID; GLYCAN; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; HYBRID PROTEIN; INTERLEUKIN 3; MILODISTIM;

AMINO ACID SEQUENCE; ARTICLE; DEAMINATION; GEL CHROMATOGRAPHY; GENETIC ENGINEERING; GENETIC HETEROGENEITY; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; MASS SPECTROMETRY; PEPTIDE MAPPING; PH ELECTRODE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; AMINO ACIDS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HYDROGEN-ION CONCENTRATION; INTERLEUKIN-3; ISOELECTRIC FOCUSING; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; POLYSACCHARIDES; PROTEIN ISOFORMS; RECOMBINANT FUSION PROTEINS;

EID: 0032781656     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-9673(99)00127-2     Document Type: Article

References (18)

1. Bishop M.R., Jackson J.D., O'Kane-Murphy B., Smith-Pokorny K., Vose J.M., Bierman P.J., Warkentin P.I., Armitage J.O., Garisson L., Kessinger A. J. Clin. Oncol. 14:1996;2521.
2. Curtis B.M., Williams D.E., Broxmeyer H.E., Dunn J., Farrah T., Jeffery E., Clevenger W., deRoos P., Martin U., Friend D., Craig V., Gayle R., Price V., Cosman D., March C.J., Park L.S. Proc. Natl. Acad. Sci. USA. 88:1991;5809.
3. Balland A., Krasts D.A., Hoch K.L., Gerhart M.J., Stremler K.E., Waugh S.M. Eur. J. Biochem. 251:1998;812.
4. Medve J., Lee D., Tjerneld F. J. Chromatogr. A. 808:1998;153.
5. Adachi T., Takyanagi H., Sharpe A.D. J. Chromatogr. A. 763:1997;57.
6. Le Berre Anton V., Gilles Bompard C., Payan F., Rouge P. Biochim. Biophys. Acta. 1343:1997;31.
7. Timosi C.M., Barrios De Tomassi J., Zambrano E., Gonzalez R., Ulloa Aguirre A. Neuroendocrinology. 67:1998;87.
8. Righetti P.G. Immobilized pH Gradients - Theory and Methodology. 1990;Elsevier, Amsterdam.
9. Righetti P.G., Bossi A., Wenisch E., Orsini G. J. Chromatogr. B. 699:1997;105.
10. Righetti P.G., Bossi A. Anal. Biochem. 247:1997;1.
11. Celentano F.C., Gianazza E., Righetti P.G. Electrophoresis. 12:1991;693.
12. Giaffreda E., Tonani C., Righetti P.G. J. Chromatogr. 630:1993;313.
13. Kitamura T., Tange T., Terasawa T., Chiba S., Kuwaki T., Miyagawa K., Piao Y.F., Miyazono K., Urabe A., Takaku F. J. Cell. Physiol. 140:1989;323.
14. Kurjan J., Herskowitz I. Cell. 30:1982;933.
15. Bordallo C., Cueva R., Suarez Rendueles P. J. Gen. Microbiol. 136:1990;419.
16. Bergwerff A.A., Stark W., Fendrich G., Knecht R., Blommers M.J.J., Maerki W., Kragten E.A., Van Oostrum J. Eur. J. Biochem. 253:1998;560.
17. Jars M.U., Osborn S., Frostrom J., MacKay V.L. J. Biol. Chem. 270:1995;24810.
18. Hecht R., Tressel T., Goldshteyn V., Winters D., Wilson J., Boone T., Daniels M. Prep. Biochem. Biotechnol. 26:1996;201.