Identification of Manα1-3Manα1-2Man and Man-linked phosphate on O- mannosylated recombinant leech-derived tryptase inhibitor produced by Saccharomyces cerevisiae and determination of the solution conformation of the mannosylated polypeptide

European Journal of Biochemistry

Volumn 253, Issue 3, 1998, Pages 560-575

  Bergwerff, Aldert A ^a   Stark, Wilhelm ^a   Fendrich, Gabriele ^a   Knecht, René ^a   Blommers, Marcel J J ^a   Maerki, Walter ^a   Kragten, Eddy A ^a   Van Oostrum, Jan ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Matrix assisted laser desorption ionization mass spectrometry; NMR; O mannosylation; Phosphorylated mannose; Yeast

Indexed keywords

ENZYME INHIBITOR; GLYCINE; MANNOSE; OLIGOSACCHARIDE; POLYPEPTIDE; SERINE; THREONINE; TRYPTASE; TRYPTASE INHIBITOR; TYROSINE; UNCLASSIFIED DRUG; VALINE; LEECH DERIVED TRYPTASE INHIBITOR C; LEECH-DERIVED TRYPTASE INHIBITOR C; OLIGOPEPTIDE; PEPTIDE FRAGMENT; PROTEIN; RECOMBINANT PROTEIN; SERINE PROTEINASE INHIBITOR;

AMINO ACID SEQUENCE; ARTICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN GLYCOSYLATION; PROTEIN ISOLATION; PROTEIN MODIFICATION; PROTEIN POLYMERIZATION; PROTEIN SECONDARY STRUCTURE; SACCHAROMYCES CEREVISIAE; ANIMAL; CARBOHYDRATE ANALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; GLYCOSYLATION; ISOLATION AND PURIFICATION; LEECH; MASS SPECTROMETRY; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; SOLUTION AND SOLUBILITY;

HIRUDINIDA; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; ANIMALS; CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; GLYCOSYLATION; LEECHES; MANNOSE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OLIGOPEPTIDES; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEINS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SERINE PROTEINASE INHIBITORS; SOLUTIONS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0032056345     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2530560.x     Document Type: Article

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