Production of biologically active, heterodimeric porcine interleukin-12 using a monocistronic baculoviral expression system

Veterinary Immunology and Immunopathology

Volumn 72, Issue 3-4, 1999, Pages 289-302

  Kokuho, T ^a   Watanabe, S ^a   Yokomizo, Y ^a   Inumaru, S ^a  

^a Meat Science Laboratory   (Japan)

Author keywords

Baculoviral expression; FMDV; Molecular bioengineering; Porcine IL 12

Indexed keywords

HYBRID PROTEIN; PROTEIN P35; PROTEIN P40; RECOMBINANT INTERLEUKIN 12; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS VECTOR;

ARTICLE; BACULOVIRUS; BIOSYNTHESIS; FOOT AND MOUTH DISEASE VIRUS; GENE EXPRESSION SYSTEM; IN VITRO STUDY; INSECT; POLYACRYLAMIDE GEL ELECTROPHORESIS; VIRUS RECOMBINANT; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; APHTHOVIRUS; BACULOVIRIDAE; DIMERIZATION; GENES, VIRAL; GENETIC VECTORS; HUMANS; INTERLEUKIN-12; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SWINE; VIRAL STRUCTURAL PROTEINS;

EID: 0032763630     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-2427(99)00141-5     Document Type: Article

References (37)

1. Baeuerle P.A., Henkel T. Function and activation of NF-κB in the immune system. Annu. Rev. Immunol. 12:1994;141-179.
2. Batson S., Rundell K. Proteolysis at the 2A/2B junction in Theiler's murine encephalomyelitis virus. Virology. 181:1991;764-767.
3. Brown C.S., Vanlent J.W., Vlak J.M., Spaan W.J.M. Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus-B19 structural proteins. J. Virol. 65:1991;2702-2706.
4. Chaplin P.J., Camon E.B., Villarreal-Ramos B., Flint M., Ryan M.D., Collins R.A. Production of interleukin-12 a self-processing 2A peptide. J. Interferon Cytokine Res. 19:1999;235-241.
5. Collins R.A., Camon E.B., Chaplin P.J., Howard C.J. Influence of IL-12 on interferon-γ production by bovine leucocyte subsets in response to bovine respiratory syncytialvirus. Vet. Immunol. Immunopath. 63:1998;69-72.
6. D'Andrea A., Rengaraju M., Valiante N.M., Chehimi J., Kubin M., Aste M., Chan S.H., Kobayashi M., Young D., Nickbarg E., Chizzonite R., Wolf S.F., Trinchieri G. Production of natural killer cell stimulatory factor (interleukin-12) by peripheral blood mononuclear cells. J. Exp. Med. 176:1992;1387-1398.
7. de Kretser D.M., Phillips D.J. Mechanisms of protein feedback on gonadotropin secretion. J. Reprod. Immunol. 39:1998;1-12.
8. French T.J., Roy P. Synthesis of bluetongue virus (BTV) corelike particles by a recombinant baculovirus expressing the two major structural core proteins of BTV. J. Virol. 64:1990;1530-1536.
9. H1 cells of bone marrow derived macrophages cultured in granulocyte macrophage colony stimulating factor macrophage colony stimulating factor. Int. Immunol. 4:1992;755-764.
10. gag virus-like particles from baculovirus-infected insect cells. Cell. 59:1989;103-112.
11. Gillessen S., Carvajal D., Ling P., Podlaski F.J., Stremlo D.L., Familletti P.C., Gubler U., Presky D.H., Stern A.S., Gately M.K. Mouse interleukin-12 (IL-12) p40 homodimer: a potent IL-12 antagonist. Eur. J. Immunol. 25:1995;200-206.
12. Gulber U., Chua A.O., Schoenhaut D.S., Dwyer C.M., McComas W., Motyka R., Nabavi N., Wolitzky A.G., Quinn P.M., Familletti P.C., Gately M.K. Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc. Natl. Acad. Sci. USA. 88:1991;4143-4147.
13. Heufler C., Koch F., Stanzl U., Topar G., Wysocka M., Trinchieri G., Enk A., Steinman R.M., Romani N., Schuler G. Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-g production by T helper 1 cells. Eur. J. Immunol. 26:1996;659-668.
14. Kobayashi M., Fitz L., Ryan M., Hewick R.M., Clark S.C., Chan S., Loudon R., Sherman F., Perussia B., Trinchieri G. Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes. J. Exp. Med. 170:1989;827-845.
15. Kokuho T., Uchimura A., Inumaru S. cDNA cloning of porcine interleukin-2 receptor-α gene. Immunol. Cell Biol. 75:1997;515-518.
16. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
17. Leischke G.J., Rao P.K., Gately M.K., Mulligan R.C. Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity in vivo. Nature Biotech. 15:1997;35-40.
18. Loudon P.T., Roy P. Assembly of five bluetongue virus proteins expressed by recombinant baculoviruses: inclusion of the largest protein VP1 in the core and virus-like particles. Virology. 180:1991;798-802.
19. Ling P., Gately M.K., Gubler U., Stern A.S., Lin P., Hollfelder K., Su C., Pan Y.E., Hakimi J. Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity. J. Immunol. 154:1995;116-127.
20. Martinotti A., Stoppacciaro A., Vagliani M., Melani C., Spreafico F., Wysocka M., Parmiani G., Trinchieri G., Colombo M.P. CD4 T cells inhibit in vivo the CD8-mediated immune response against murine colon carcinoma cells transduced with interleukin-12 genes. Eur. J. Immunol. 25:1995;137-146.
21. Mattner F., Fisher S., Guckes S., Jin S., Kaulen H., Schmitt E., Rude E., Germann T. The interleukin-12 subunit p40 specifically inhibits effects of the interleukin-12 heterodimer. Eur. J. Immunol. 23:1993;2202-2208.
22. Montross L., Watkins S., Moreland R.B., Mamon H., Casper D.L.D., Garcea R.L. Nuclear assembly of polyomavirus capsids in insect cells expressing the major capsid protein VP1. J. Virol. 65:1991;4991-4998.
23. Palmenberg A.C. Proteolytic processing of picornaviral polyprotein. Annu. Rev. Microbiol. 44:1990;603-623.
24. Percy N., Barclay W.S., Garcia-Sastre A., Palese P. Expression of a foreign protein by influenza A virus. J. Virol. 68:1994;4486-4492.
25. Presky D.H., Minnetti L.J., Gillessen S., Willkinson V.L., Wu C.Y., Gubler U., Chizzonite R., Gately M.K. Analysis of the multiple interactions between IL-12 and the high affinity IL-12 receptor complex. J. Immunol. 160:1998;2147-2179.
26. Robertson B.H., Grubman M.J., Weddell G.N., Moore D.M., Welsh J.D., Fischer T., Dowbenko D.J., Yansura D.G., Small B., Kleid D.G. Nucleotide and amino acid sequence coding for polypeptides of foot-and-mouth disease virus type A12. J. Virol. 54:1985;651-660.
27. Ryan M.D., Belsham G.J., King A.M.Q. Specificity of enzyme-substrate interactions in foot-and-mouth disease virus polyprotein processing. Virology. 173:1989;35-45.
28. Ryan M.D., King A.M.Q., Thomas G.P. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence. J. Gen. Virol. 72:1991;2727-2732.
29. Ryan M.D., Drew J. Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J. 13:1994;928-933.
30. Schoenhaut D.S., Chua A.O., Wolitzky A.G., Quinn P.M., Dwyer C.M., McComas M., Familletti P.C., Gately M.K., Gubler U. Cloning and expression of murine IL-12. J. Immunol. 148:1992;3433-3440.
31. Scott P. IL-12: initiation cytokine for cell-mediated immunity. Science. 260:1993;496-497.
32. Stern A.S., Podlaski F.J., Hulmes J.D., Pan Y.E., Quinn P.M., Wolitzky A.G., Familletti P.C., Stremlo D.L., Trutti T., Chizzonite R., Gately M.K. Purification to homogeneity and partial characterization of cytotoxic lymphocyte maturation factor from human B-lymphoblastoid cells. Proc. Natl. Acad. Sci. USA. 87:1990;6808-6812.
33. H1 cells. Immunol. Today. 14:1993;335-338.
34. Trinchieri G. Interleukin-12: a proinflammatory cytokine with immunoregulatory functions that bridge innate resistance and antigen-specific adaptive immunity. Annu. Rev. Immunol. 13:1995;251-276.
35. Wang X., Wilkinson V.L., Podlaski F.J., Wu C.Y., Stern A.S., Presky D.H., Magram J. Characterization of mouse interleukin-12 p40 homodimer binding to the interleukin-12 receptor subunits. Eur. J. Immunol. 29:1999;2007-2013.
36. Wolf S.F., Temple P.A., Kobayashi M., Young D., Dicig M., Lowe L., Dzialo R., Fitz L., Ferenz C., Hewick R.M., Kelleher K., Herrmann S.H., Clark S.C., Azzoni L., Chan S.H., Trinchieri G., Perussia B. Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J. Immunol. 146:1991;3074-3081.
37. Zitvogel L., Tahara H., Cai Q., Storkus W.J., Muller G., Wolf S.F., Gately M.K., Robbins P.D., Lotze M.T. Construction and characterization of retroviral vector expressing biological active human interleukin-12. Human Gene Ther. 5:1994;1493-1506.