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Volumn 71, Issue 22, 1999, Pages 5137-5145

Single-molecule detection of DNA separations in microfabricated capillary electrophoresis chips employing focused molecular streams

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CAPILLARY ELECTROPHORESIS; DIAGNOSTIC ACCURACY; DNA DETERMINATION; FLUORESCENCE; MOLECULAR RECOGNITION;

EID: 0032753438     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac990644t     Document Type: Article
Times cited : (139)

References (33)
  • 27
    • 84920355960 scopus 로고    scopus 로고
    • note
    • A correction has to made for changes in the separation current with focus current Since the separation current decreases with increasing focus current, the rate of introduction of molecules to the detection region also decreases. However, in this experiment the separation current changed by only 10%, and the effect on molecules counted was not noticeable over the 25-s data set.
  • 29
    • 84920353754 scopus 로고    scopus 로고
    • note
    • The signal-to-noise ratio of a peak was calculated by the formula (S - B)/ sqrt(B), where S is the peak height and B is the average background in the vicinity of the peak. The values used for the calculations in Figure 10A were as follows: S = 560 and B = 280 (analog); S = 53 and B = 3 (singlemolecule counting). The values used for the calculations in Figure 10B were as follows: S = 490 and B = 310 (analog); S = 40 and B = 3 (single-molecule counting).
  • 30
    • 84920359570 scopus 로고    scopus 로고
    • note
    • The sample was injected from 0.01X TAE into IX TAE. Taking into account diffusion and equilibration of buffer concentrations upon injection, the actual concentration enhancement due to sample stacking was estimated to be 10-fold.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.