Reduction of luminescent background in ultrasensitive fluorescence detection by photobleaching

Analytical Chemistry

Volumn 68, Issue 13, 1996, Pages 2270-2276

  Affleck, Rhett L ^a,c   Ambrose, W Patrick ^a   Demas, James N ^b   Goodwin, Peter M ^a   Schecker, Jay A ^a   Wu, Ming ^a,d   Keller, Richard A ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c PHARMACOPEIA INC   (United States)

^d BROOKHAVEN NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL ANALYSIS; IMPURITIES; LUMINESCENCE; SOLVENTS;

DETECTION LIMITS; FLUORESCENCE DETECTION; PHOTOBLEACHING;

FLUORESCENCE;

EID: 0030180971     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac9512517     Document Type: Article

References (24)

1. Shera, E. B.; Seitzinger, N. K.; Davis, L. N.; Keller, R. A.; Soper, S. A. Chem. Phys. Lett. 1990, 174, 553-557.
2. Soper, S. A.; Shera, E. B.; Martin, J. C.; Jett, J. H.; Hahn. J. H.; Nutter, H. L.; Keller, R. A. Anal. Chem. 1991, 63, 432-437.
3. Soper, S. A.; Davis, L. M.; Shera, E. B. J. Opt. Soc. Am. B 1992, 9, 1761-1769.
4. Whitten, W. B.; Ramsey, J. M.; Arnold, S.; Bronk, B. V. Anal. Chem. 1991, 63, 1027-1031.
5. Soper, S. A.; Mattingly, Q. L.; Vegunta, P. Anal. Chem. 1993, 65, 740-747.
6. Wilkerson, C. W.; Goodwin, P. M.; Ambrose, W. P.; Martin, J. C.; Keller, R. A. Appl. Phys. Lett. 1993, 62, 2030-2032.
7. Lee, Y.-H.; Maus, R. G.; Smith, B. W.; Winefordner, J. D. Anal. Chem. 1994, 66, 4142-4149.
8. Rigler, R.; Widengren, J.; Mets, U. In Fluorescence Spectroscopy; Wolfbeis, O. S., Ed.; Springer-Verlag: Berlin, 1993; pp 13-24.
9. Mets, U.; Rigler, R. J. Fluor. 1994, 4, 259-264.
10. Nie, S.; Chiu, D. T.; Zare, R. N. Science 1994, 266, 1018-1021.
11. Nie, S.; Chiu, D. T.; Zare, R. N. Anal. Chem. 1995, 67, 2849-2857.
12. Li, L.-Q.; Davis, L. M. Appl. Opt. 1995, 34, 3208-3217.
13. Shapiro, H. M. Practical Flow Cytometry; Wiley-Liss: New York, 1995; pp 133-143.
14. Cheng, Y. F.; Wu, S.; Chen, D. Y.; Dovichi, N. J. Anal. Chem. 1990, 62, 496-503.
15. Ambrose, W. P.; Goodwin, P. M.; Jett, J. H.; Johnson, M. E.; Martin, J. C.; Marrone, B. L.; Schecker, J. A.; Wilkerson, C. W.; Keller, R. A.; Haces A.; Shih, P.-J.; Harding J. D. Ber. Bunsenges. Phys. Chem. 1993, 97, 1535-1542.
16. Schecker, J. A.; Goodwin, P. M.; Affleck, R. L.; Wu, M.; Martin, J. C.; Jett, J. H.; Keller, R. A.; Harding, J. D. Proc. SPIE-Int. Soc. Opt. Eng. 1995, 2386, 4-12.
17. Goodwin, P. M.; Affleck, R. L.; Ambrose, W. P.; Demas, J. N.; Jett, J. H.; Martin, J. C.; Reha-Krantz, L. J.; Semin, D. J.; Schecker, J. A.; Wu, M; Keller, R. A. Exp. Tech. Phys., in press.
18. Goodwin, P. M.; Wilkerson, C. W., Jr.; Ambrose, W. P.; Keller. R. A. Proc. SPIE-Int. Soc. Opt. Eng. 1993, 1895, 79-89.
19. Harris, T. D.; Lytle, F. E. In Ultrasensitive Laser Spectroscopy; Kliger, D. S., Ed.; Academic Press: New York, 1983; pp 343-367.
20. Peck, K.; Stryer, L.; Glazer, A. N.; Mathies, R. A. Proc. Natl. Acad. Sci. U.S.A. 1989, 86, 4087-4091.
21. White, J. C.; Stryer, L. Anal. Biochem. 1987, 161, 442-452.
22. Mathies, R. A.; Stryer, L. In Applications of Fluorescence in Biological Sciences; Taylor, D. L., Waggoner, A. S., Lanni, F., Murphy, R. F., Birge, R. R., Eds.; Alan R. Reiss, Inc.: New York, 1986; pp 129-140.
23. Soper, S. A.; Nutter, H. L.; Keller, R. A.; Davis, L. M.; Shera, E. B. Photochem. Photobiol. 1993, 57, 972-977.
24. Yappert, M. C., Department of Chemistry, University of Louisville, Louisville, KY 40292. Private communication, March 1996.