Expression of human β3-adrenergic receptor induces adipocyte-like features in CHO/K1 fibroblasts

Journal of Cell Science

Volumn 112, Issue 21, 1999, Pages 3791-3797

  Gros, Jérôme ^a   Gerhardt, Cindy C ^a   Strosberg, A Donny ^a  

^a UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

AR; Adipocyte; Adipogenesis; Adrenergic receptor; CHO; PPAR

Indexed keywords

ARGININE; BETA 3 ADRENERGIC RECEPTOR; BUPRANOLOL; FAT DROPLET; LEPTIN; MESSENGER RNA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE; TRYPTOPHAN; UNCOUPLING PROTEIN;

ADIPOCYTE; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; DNA POLYMORPHISM; FIBROBLAST; GENETIC TRANSFECTION; HUMAN; LIPID STORAGE; MORBID OBESITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REGULATOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADIPOCYTES; ANIMALS; AZO COMPOUNDS; CELL DIFFERENTIATION; CHO CELLS; COLORING AGENTS; CRICETINAE; CRICETULUS; FIBROBLASTS; GENE EXPRESSION REGULATION; HUMANS; LIPID METABOLISM; RECEPTORS, ADRENERGIC, BETA; RECEPTORS, ADRENERGIC, BETA-3; RECEPTORS, CYTOPLASMIC AND NUCLEAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION FACTORS;

ANIMALIA; EUKARYOTA;

EID: 0032716745     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Aperlo, C., Pognonec, P., Saladin, R., Auwerx, J. and Boulukos, K. E. (1995). cDNA cloning and characterization of the transcriptional activities of the hamster peroxisome proliferator-activated receptor haPPAR gamma. Gene 162, 297-302.
2. Arch, J. R. S. and Wilson, S. (1996). beta(3)-adrenoceptors and the regulation of metabolism in adipose tissues. Biochem. Soc. Trans. 24, 412-418.
3. Auwerx, J., Martin, G., Guerremillo, M. and Staels, B. (1996). Transcription, adipocyte differentiation, and obesity. J. Mol. Med. 74, 347-352.
4. Braissant, O., Foufelle, F., Scotto, C., Dauca, M. and Wahli, W. (1996). Differential expression of peroxisome proliferator-activated receptors (PPARs): tissue distribution of PPAR-alpha, -beta, and -gamma in the adult rat. Endocrinology 137, 354-366.
5. Bronnikov, G., Houstek, J. and Nedergaard, J. (1992). Beta-adrenergic, cAMP-mediated stimulation of proliferation of brown fat cells in primary culture. Mediation via beta 1 but not via beta 3 adrenoceptors. J. Biol. Chem. 267, 2006-2013.
6. Candelore, M. R., Deng, L. P., Tola, L. M., Kelly, L. J., Cascieri, M. A. and Strader, C. D. (1996). Pharmacological characterization of a recently described human beta 3-adrenergic receptor mutant. Endocrinology 137, 2638-2641.
7. Chawla, A., Schwarz, E. J., Dimaculangan, D. D. and Lazar, M. A. (1994). Peroxisome proliferator-activated receptor (PPAR) gamma: adipose-predominant expression and induction early in adipocyte differentiation. Endocrinology 135, 798-800.
8. Christoffersen, C. T., Tornqvist, H., Vlahos, C. J., Bucchini, D., Jami, J., De Meyts, P. and Joshi, R. L. (1998). Insulin and insulin-like growth factor-1 receptor mediated differentiation of 3T3-F442A cells into adipocytes: effects of P13-kinase inhibition. Biochem. Biophys. Res. Commun. 246, 426-430.
9. Cornelius, P., MacDougald, O. A. and Lane, M. D. (1994). Regulation of adipocyte development. Annu. Rev. Nutr. 14, 99-129.
10. Echtay, K. S., Bienengraeber, M. and Klingenberg, M. (1997). Mutagenesis of the uncoupling protein of brown adipose tissue. Biochemistry 36, 8253-8260.
11. Fajas, L., Fruchart, J. C. and Auwerx, J. (1998). Transcriptional control of adipogenesis. Curr. Opin. Cell Biol. 10, 165-173.
12. Forman, B. M., Chen, J. and Evans, R. M. (1996). The peroxisome proliferator-activated receptors: ligands and activators. Ann. NY Acad. Sci. 804, 266-275.
13. Gerhardt, C. C., Gros, J., Strosberg, A. D. and Issad, T. (1999). Stimulation of the ERK1/2 pathway by the human beta 3 adrenergic receptor: new pharmacological profile and mechanism of activation. Mol. Pharmacol. 55, 255-262.
14. Ghorbani, M., Claus, T. H. and HimmsHagen, J. (1997). Hypertrophy of brown adipocytes in brown and white adipose tissues and reversal of diet-induced obesity in rats treated with a beta(3)-adrenoceplor agonist. Biochem. Pharmacol. 54, 121-131.
15. Gimble, J. M., Robinson, C. E., Wu, X., Kelly, K. A., Rodriguez, B. R., Kliewer, S. A., Lehmann, J. M. and Morris, D. C. (1996). Peroxisome proliferator-activated receptor-gamma activation by thiazolidinediones induces adipogenesis in bone marrow stromal cells. Mol. Pharmacol. 50, 1087-1094.
16. Green, H. and Kehinde, O. (1974). Sublines of mouse 3T3 cells that accumulate lipid. Cell 1, 113-116.
17. Green, H. and Kehinde, O. (1976). Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells. Cell 7, 105-113.
18. Gregoire, F. M., Smas, C. M. and Sul, H. S. (1998). Understanding adipocyte differentiation. Physiol. Rev. 78, 783-809.
19. Gros, J., Manning, B. S., Pietri-Rouxel, F., Guillaume, J. L., Drumare, \ M. F. and Strosberg, A. D. (1998). Site-directed mutagenesis of the human beta3-adrenoceptor:transmembrane residues involved in ligand binding and signal transduction. Eur. J. Biochem. 251, 590-596.
20. Himms-Hagen, J. (1990). Brown adipose tissue thermogenesis: interdisciplinary studies. FASEB J. 4, 2890-2898.
21. Lambe, K. G. and Tugwood, J. D. (1996). A human peroxisome-proliferator-activated receptor-gamma is activated by inducers of adipogenesis, including thiazolidinedione drugs. Eur. J. Biochem. 239, 1-7.
22. Li, L. S., Lonnqvist, F., Luthman, H. and Arner, P. (1996). Phenotypic characterization of the Trp64Arg polymorphism in the beta(3)-adrenergic receptor gene in normal weight and obese subjects. Diabetologia 39, 857-860.
23. Maffei, M., Fei, H., Lee, G. H., Dani, C., Leroy, P., Zhang, Y., Proenca, R., Negrel, R., Ailhaud, G. and Friedman, J. M. (1995). Increased expression in adipocytes of ob RNA in mice with lesions of the hypothalamus and with mutations at the db locus. Proc. Nat. Acad. Sci. USA 92, 6957-6960.
24. Magun, R., Burgering, B. M., Coffer, P. J., Pardasani, D., Lin, Y., Chabot, J. and Sorisky, A. (1996). Expression of a constitutively activated form of protein kinase B (c-Akt) in 3T3-LI preadipose cells causes spontaneous differentiation. Endocrinology 137, 3590-3593.
25. Moule, S. K., Welsh, G. I., Edgell, N. J., Foulstone, E. J., Proud, C. G. and Denton, R. M. (1997). Regulation of protein kinase B and glycogen synthase kinase-3 by insulin and beta-adrenergic agonists in rat epididymal fat cells. Activation of protein kinase B by wortmannin-sensitive and -insensitive mechanisms. J. Biol. Chem. 272, 7713-7719.
26. Piétri-Rouxel, F., Manning, B., Gros, J. and Strosberg, A. D. (1997). The biochemical effect of the naturally occurring Trp64Arg mutation on human β3-adrenoceptor activity. Eur. J. Biochem. 247, 1174-1179.
27. Ramirez-Zacarias, J. L., Castro-Munozledo, F. and Kuri-Harcuch, W. (1992). Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97, 493-497.
28. Rentsch, J. and Chiesi, M. (1996). Regulation of ob gene mRNA levels in cultured adipocytes. FEBS Lett. 379, 55-59.
29. Sale, E. M., Atkinson, P. G. and Sale, G. J. (1995). Requirement of MAP kinase for differentiation of fibroblasts to adipocytes, for insulin activation of p90 S6 kinase and for insulin or serum stimulation of DNA synthesis. EMBO J. 14, 674-684.
30. Shimizu, Y., Tanishita, T., Minokoshi, Y. and Shimazu, T. (1997). Activation of mitogen-activated protein kinase by norepinephrine in brown adipocytes from rats. Endocrinology 138, 248-253.
31. Snitker, S., Odeleye, O. E., Hellmer, J., Boschmann, M., Monroe, M. B., Shuldiner, A. R. and Ravussin, E. (1997). No effect of the Trp64Arg beta 3-adrenoceptor variant on in vivo lipolysis in subcutaneous adipose tissue. Diabetologia 40, 838-842.
32. Spiegelman, B. M. and Flier, J. S. (1996). Adipogenesis and obesity: rounding out the big picture. Cell 87, 377-389.
33. Strosberg, A. D. and Pietri-Rouxel, F. (1996). Function and regulation of the beta(3)-adrenoceptor. Trends Pharmacol. Sci. 17, 373-381.
34. Strosberg, A. D. (1997). Association of beta 3-adrenoceptor polymorphism with obesity and diabetes: current status. Trends Pharmacol. Sci. 18, 449-454.
35. Tontonoz, P., Hu, E., Graves, R. A., Budavari, A. I. and Spiegelman, B. M. (1994a). mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer. Genes Dev. 8, 1224-1234.
36. Tontonoz, P., Hu, E. and Spiegelman, B. M. (1994b). Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor. Cell 79, 1147-1156.
37. Zilberfarb, V., Pietri-Rouxel, F., Jockers, R., Krief, S., Delouis, C., Issad, T. and Strosberg, A. D. (1997). Human immortalized brown adipocytes express functional beta3-adrenoceptor coupled to lipolysis. J. Cell Sci. 110, 801-807.