Transfections of primary muscle cell cultures with plasmids coding for GFP linked to full-length and truncated muscle proteins

Methods in Cell Biology

Volumn , Issue 58, 1999, Pages 239-260

  Dabiri, Guissou A ^a   Turnacioglu, Kenan K ^a   Ayoob, Joseph C ^a   Sanger, Jean M ^a   Sanger, Joseph W ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MUSCLE PROTEIN; PHOTOPROTEIN;

ANIMAL; BIRD; CELL CULTURE; GENETIC TRANSFECTION; GENETICS; HEART; HEART MUSCLE; METABOLISM; PLASMID; PRENATAL DEVELOPMENT; REVIEW; SARCOMERE; SKELETAL MUSCLE; ULTRASTRUCTURE;

ANIMALS; BIRDS; CELLS, CULTURED; GREEN FLUORESCENT PROTEINS; HEART; LUMINESCENT PROTEINS; MUSCLE PROTEINS; MUSCLE, SKELETAL; MYOCARDIUM; PLASMIDS; RECOMBINANT FUSION PROTEINS; SARCOMERES; TRANSFECTION;

EID: 0032608582     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (44)

1. Arimura, C., Suzuki, T., Yanagisawa, M., Immura, M., Hamada, Y., and Masaki, T. (1988). Primary structure of chicken muscle and fibroblast alpha-actinins deduced from cDNA sequences. Eur. J. Biochem. 177, 649-655.
2. Bischoff, R., and Holtzer, H. (1968). The effect of mitotic inhibitors on myogenesis in vitro. J. Cell Biol. 36, 111-127.
3. Capecchi, M. R. (1980). High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell 22, 479-488.
4. Cavanaugh, M. W. (1955). Pulsation, migration and division in dissociated chick embryo heart cells in vitro. J. Exp. Zool. 128, 573-590.
5. Chacko, S. (1973). DNA synthesis, mitosis and differentiation in cardiac myogenesis. Develop. Biol. 35, 1-18.
6. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., and Prasher, D. C. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
7. Cole, N. B., Smith, C. L., Sciaky, N., Terasaki, M., Edidin M., and Lippincott-Schwartz, J. (1996). Diffusional mobility of Golgi proteins in membranes of living cells. Science 273, 797-801.
8. Dabiri, G. A., Turnacioglu, K. K., Sanger, J. M., and Sanger, J. W. (1997). Myofibrillogenesis visualized in living embryonic cardiomyocytes. Proc. Natl. Acad. Sci. U.S.A. 94, 9493-9498.
9. DeHaan, R. L. (1968). Emergence of form and function in the embryonic heart. Develop. Biol. Suppl. 2, 208-250.
10. Dome, J. S., Mittal, B., Pochapin, M. B., Sanger J. M., and Sanger, J. W. (1988). Incorporation of fluorescently labeled actin and tropomyosin into muscle cells. Cell Differ. 23, 37-52.
11. Doyle, T., and Botstein, D. (1996). Movement of yeast cortical actin cytoskeleton in vivo. Proc. Natl. Acad. Sci. U.S.A. 93, 3886-3891.
12. Epstein, H. F., and Fischman, D. A. (1991). Molecular analysis of protein assembly in muscle development. Science 251, 1039-1044.
13. Fischbach, G. D. (1972). Synapse formation between dissociated nerve and muscle in low density cell cultures. Develop. Biol. 28, 407-429.
14. Higley, S., and Way, M. (1997). Actin and cell pathogenesis. Curr. Opin. Cell Biol. 9, 62-69.
15. Gerisch, G., Albrecht, R., Heizer, C., Hodgkinson, S., and Maniak, M. (1995). Chemoattractant-controlled accumulation of coronin at the leading edge of Dictyostelium cells monitored using a green fluorescent protein-coronin fusion protein. Curr. Biol. 5, 1280-1285.
16. Inoué, S., and K. R. Spring. (1997). "Video Microscopy: The Fundamentals," pp. 1-741. Plenum Press, New York.
17. Kreis, T. E., Avnur, Z., Schlessinger, J., and Geiger, B. (1984). Dynamic properties of cytoskeletal proteins in focal contacts. In "Molecular Biology of the Cytoskelton" (Borisy, G. G., Cleveland, D., and Murphy, D. B., Eds.), pp. 45-57. Cold Spring Harbor Press, Cold Spring Harbor, NY.
18. Labeit, S., and Kolmerer, B. (1995). Titins: giant proteins in charge of muscle ultrastructure and elasticity. Science 270, 293-296.
19. Mittal, B., Sanger, J. M., and Sanger, J. W. (1987). Visualization of myosin in living cells. J. Cell Biol. 105, 1753-1760.
20. Mittal, B., Danowski, B. A., Sanger, J. M., and Sanger, J. W. (1992). Expression of desmin cDNA in PtK2 cells results in assembly of desmin filaments from multiple sites throughout the cell. Cell Motil. Cytoskel. 23, 188-200.
21. Moores, S. L., Sabry, J. H., and Spudich, J. A. (1996). Myosin dynamics in live Dictyostelium cells. Proc. Natl. Acad. Sci. U.S.A. 93, 443-446.
22. Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., and Remington, S. J. (1996). Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392-1395.
23. Pochapin, M. B., Sanger J. M., and Sanger, J. W. (1983). Microinjection of Lucifer Yellow CH into sea urchin eggs and embryos. Cell Tissue Res. 234, 309-318.
24. Rhee, D., Sanger, J. M., and Sanger, J. W. (1994). The premyofibril: evidence for its role in myofibrillogenesis. Cell Motil. Cytoskel. 28, 1-24.
25. Rizzuto, R., Brini, M., Pizzo, P., Murgia, M., and Pozzan, T. (1995). Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr. Biol. 5, 635-642.
26. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). "Molecular Cloning: A Laboratory Manual." Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY.
27. Sanger, J. W. (1977). Mitosis in beating cardiac myoblasts treated with cytochalasin-B. J. Exp. Zool. 201, 463-469.
28. Sanger, J. M., Pochapin, M. B., and Sanger, J. W. (1985). Midbody sealing after cytokinesis in embryos of the sea urchin, Arbacia punctulata. Cell Tissue Res. 240, 287-292.
29. Sanger, J. M., Mittal, B., Pochapin, M. B., and Sanger, J. W. (1986). Myofibrillogenesis in living cells microinjected with fluorescently labeled alpha-actinin. J. Cell Biol. 102, 2053-2066.
30. Sanger, J. M., Mittal, B., Dome, J. B., and Sanger, J. W. (1989). Analysis of cell division using fluorescently labeled actin and myosin in living cells. Cell Motil. Cytoskel. 14, 201-219.
31. Sanger, J. M., Rhee, D., Leonard, M., Price, M., Zhukarev, V., Shuman, H., and Sanger, J. W. (1994a). Assembly of myofibrils and cleavage furrows in cardiomyocytes. Mol. Biol. Cell 5: 165a.
32. Sanger, J. M., Dome, J. S., Hock, R. S., Mittal, B., and Sanger, J. W. (1994b). The occurrence of contractile fibers and their association with talin in the cleavage furrow in living PtK2 cells. Cell Motil. Cytoskel. 27, 26-40.
33. Sanger, J. M., Golla, R., Safer, D., Choi, J. K., Yu, K., Sanger, J. W., and Nachmias, V. T. (1995). Increasing intracellular concentrations of thymosinbeta4 in PtK2 cells: effects on stress fibers, cytokinesis and cell spreading. Cell Motil. Cytoskel. 31, 307-322.
34. Schultheiss, T., Lin, Z., Ishikawa, H., Zamir, I., Stoeckert C. J., and Holtzer, H. (1991). Desmin/ vimentin intermediate filaments are dispensable for many aspects of myogenesis. J. Cell Biol. 114, 953-966.
35. Stearns, T. (1995). The green revolution. Curr. Biol. 5, 262-264.
36. Theriot, J. A., and Mitchison, T. J. (1992). The nucleation-release model of actin filament dynamics in cell motility. Trends Cell Biol. 1, 219-222.
37. Turnacioglu, K. K., Mittal, B., Sanger, J. M., and Sanger, J. W. (1996). Partial characterization and DNA sequence of zeugmatin. Cell Motil. Cytoskel. 34, 108-121.
38. Turnacioglu, K. K., Mittal, B., Dabiri, G. A., Sanger, J. M., and Sanger, J. W. (1997a). Connectin (titin) and the formation of myofibrils. Cell Struct. Funct. 22, 73-82.
39. Turnacioglu, K. K., Mittal, B., Dabiri, G. A., Sanger, J. M., and Sanger, J. W. (1997b). An N-terminal fragment of titin coupled to Green Fluorescent Protein localizes to the Z-Bands in living muscle cells: overexpression leads to myofibril disassembly. Mol. Biol. Cell 8, 705-717.
40. Wang, Y.-L and Taylor, D. L. (Eds.) (1989). Fluorescence microscopy of living cells in culture part a. Fluorescent analogs, labeling cells and basic microscopy. Methods Cell Biol. 29, 1-328.
41. Yaffee, D. (1968). Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc. Natl. Acad. Sci. U.S.A. 61, 477-483.
42. Yang, F., Moss, L. G., and Phillips, G. N. (1996). The molecular structure of green fluorescent protein. Nat. Biotechnol. 14: 1246-1251.
43. Zavodny, P. J., Petro, M. E., Kumar, C. C., Daily, S. H., Lonial, H. K., Narula, S. K., and Leibowitz, P. J. (1988). The nucleotide sequence of chicken smooth muscle myosin light chain two. Nucleic Acids Res. 16, 1214.
44. Zhukarev, V. Sanger, J. M., Sanger, J. W., Goldman, Y. E., and Shuman, H. (1997). Distribution and orientation of rhodamine-phalloidin bound to thin filaments in skeletal and cardiac myofibrils. Cell Motil. Cytoskel. 37, 363-377.