Cloning and characterization of two rhamnogalacturonan hydrolase genes from Aspergillus niger

Applied and Environmental Microbiology

Volumn 63, Issue 7, 1997, Pages 2507-2515

  Suykerbuyk, Marjon E G ^a   Kester, Harry C M ^a   Schaap, Peter J ^a   Stam, Hein ^b   Musters, Wouter ^c   Visser, Jaap ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b Givaudan SA   (Switzerland)

^c UNILEVER RESEARCH VLAARDINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

GALACTURONIC ACID; HYDROLASE;

ARTICLE; ASPERGILLUS NIGER; ENZYME SPECIFICITY; FUNGAL GENETICS; FUNGUS GROWTH; MOLECULAR CLONING; NONHUMAN; SEQUENCE HOMOLOGY;

ASPERGILLUS ACULEATUS; ASPERGILLUS AWAMORI; ASPERGILLUS NIGER; FUNGI; MALUS X DOMESTICA;

EID: 0030762752     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.7.2507-2515.1997     Document Type: Article

References (34)

1. An, J., L. Zhang, M. A. O'Neill, P. Albersheim, and A. G. Darvill. 1994. Isolation and structural characterization of endo-rhamnogalacturonase-generated fragments of the backbone of rhamnogalacturonan I. Carbohydr. Res. 264:83-96.
2. Bussink, H. J. D., F. P. Buxton, B. A. Fraaye, L. H. de Graaff, and J. Visser. 1992. The polygalacturonases of Aspergillus niger are encoded by a family of diverged genes. Eur. J. Biochem. 208:83-90.
3. Cubero, B., and C. Scazzocchio. 1994. Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J. 13:407-415.
4. De Graaff, L. H., H. W. J. van den Broek, and J. Visser. 1988. Isolation and transformation of the pyruvate kinase gene of Aspergillus nidulans. Curr. Genet. 13:315-321.
5. Gouka, R. J., J. G. M. Hessing, H. Stam, W. Musters, and C. A. M. J. J. van den Hondel. 1995. A novel strategy for the isolation of defined pyrG mutants and the development of a site-specific integration system for Aspergillus awamori. Curr. Genet. 27:536-540.
6. Harmsen, J. A. M., M. A. Kusters-van Someren, and J. Visser. 1990. Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): indications of a pectin lyase gene family in Aspergillus niger. Curr. Genet. 18: 161-166.
7. Hessing, J. G. M., C. van Rotterdam, J. M. A. Verbakel, M. Roza, J. Maat, R. F. M. van Gorcom, and C. A. M. J. J. van den Hondel. 1994. Isolation and characterization of a 1,4-β-endoxylanase gene of A. awamori. Curr. Genet. 26:228-232.
8. Horton, R. M., H. D. Hunt, S. N. Ho, J. K. Pullen, and L. R. Pease. 1989. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68.
9. Irniger, S., and G. Braus. 1994. Saturation mutagenesis of a polyadenylylation signal reveals a hexanucleotide element essential for mRNA 3′ end formation in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 91:257-261.
10. Kobata, A. 1992. Structures and functions of the sugar chains of glycoproteins. Eur. J. Biochem. 209:483-501.
11. Kofod, L. V., S. Kauppinen, S. Christgau, L. N. Andersen, H. P. Heldt-Hansen, K. Dörreich, and H. Dalbøge. 1994. Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus. J. Biol. Chem. 269:29182-29189.
12. Kozak, M. 1989. The scanning model for translation: an update. J. Cell. Biol. 108:229-241.
13. Kulmburg, P., M. Mathieu, C. Dowzer, J. Kelly, and B. Felenbok. 1993. Specific binding sites in the alcR and alcA promoters of the ethanol regulon for the CREA repressor mediating carbon catabolite repression in Aspergillus nidulans. Mol. Microbiol. 7:847-857.
14. Kusters-van Someren, M. A., J. A. M. Harmsen, H. C. M. Kester, and J. Visser. 1990. Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans. Curr. Genet. 20:293-299.
15. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
16. Lau, J. M., M. McNeil, A. G. Darvill, and P. Albersheim. 1985. Structure of the backbone of rhamnogalacturonan I, a pectic polysaccharide in the primary cell walls of plants. Carbohydr. Res. 137:111-125.
17. Mutter, M., I. J. Colquhoun, H. V. Schols, G. Beldman, and A. G. J. Voragen. 1996. Rhamnogalacturonan α-L-rhamnopyranosyl-(1,4)-α-D-galactopyranosyluronide lyase. Plant Physiol. 110:73-77.
18. O'Neill, M., P. Albersheim, and A. G. Darvill. 1990. The pectic polysaccharides of primary cell walls, p. 415-141. In P. M. Dey and J. B. Harborne (ed.), Methods in plant biochemistry, vol. 2. Carbohydrates. Academic Press, London, United Kingdom.
19. Pontecorvo, G., J. A. Roper, L. J. Hemmons, K. D. MacDonald, and A. W. J. Bufton. 1953. The genetics of Aspergillus nidulans. Adv. Genet. 5:141-238.
20. Puvanesarajah, V., A. G. Darvill, and P. Albersheim. 1991. Structural characterization of two oligosaccharide fragments formed by the selective cleavage of rhamnogalacturonan II: evidence for the anomeric configuration and attachment sites of apiose and 3-deoxy-2-heptulosaric acid. Carbohydr. Res. 218:211-222.
21. Sakamoto, M., Y. Shirane, I. Naribayashi, K. Kimura, N. Morishita, T. Sakamoto, and T. Sakai. 1994. Purification and characterization of a rhamnogalacturonase with protopectinase activity from Trametes sanguinea. Eur. J. Biochem. 226:285-291.
22. Sambrook, J., E. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
23. +-dependent glutamate dehydrogenase (gdhA) from Agaricus bisporus. Mol. Gen. Genet. 250:339-347.
24. Schols, H. A., C. J. M. Geraeds, M. F. Searle-van Leeuwen, F. J. M. Kormelink, and A. G. J. Voragen. 1990. Rhamnogalacturonase: a novel enzyme that degrades the hairy regions of pectins. Carbohydr. Res. 206:105-115.
25. Schols, H. A., and A. G. J. Voragen. 1994. Occurrence of pectic hairy regions in various plant cell wall materials and their degradability by rhamnogalacturonase. Carbohydr. Res. 256:83-95.
26. Schols, H. A., A. G. J. Voragen, and I. J. Colquhoun. 1994. Isolation and characterization of rhamnogalacturonan oligomers, liberated during degradation of pectic hairy regions by rhamnogalacturonase. Carbohydr. Res. 256:97-111.
27. Searle-van Leeuwen, M. J. F., L. A. M. van den Broek, H. A. Schols, G. Beldman, and A. G. J. Voragen. 1992. Rhamnogalacturonan acetylesterase: a novel enzyme from Aspergillus aculeatus, specific for the deacetylation of hairy (ramified) regions of pectins. Appl. Microbiol. Biotechnol. 38:347-349.
28. Suykerbuyk, M. E. G., P. J. Schaap, H. Stam, W. Musters, and J. Visser. 1995. Cloning, sequence and expression of the gene coding for rhamnogalacturonase (RHG) of Aspergillus aculeatus; a novel pectinolytic enzyme. Appl. Microbiol. Biotechnol. 43:861-870.
29. Unkles, S. E. 1992. Gene organization in industrial filamentous fungi, p. 28-53. In J. R. Kinghorn and G. Turner (ed.), Applied molecular genetics of filamentous fungi. Chapman, London, United Kingdom.
30. Van den Hombergh, J. P. T. W. 1996. EMBL accession no. X96734.
31. Visser, J., H. J. Bussink, and C. Witteveen. 1994. Gene expression in filamentous fungi; expression of pectinases and glucose oxidase in Aspergillus niger, p. 241-308. In A. Smith (ed.), Gene expression in recombinant microorganisms. Marcel Dekker, Inc., New York, N.Y.
32. Von Heijne, G. 1986. A new method for predicting signal sequence cleavage sites. Nucleic Acids. Res. 14:4683-4690.
33. Whitaker, J. R. 1984. Pectic substances, pectic enzymes and haze formation in fruit juices. Enzyme Microb. Technol. 6:341-349. 33.
34. Witteveen, C. F. B., P. J. I. van de Vondervoort, H. C. van den Broeck, F. A. C. van Engelenburg, L. H. de Graaff, M. H. B. C. Hillebrand, P. J. Schaap, and J. Visser. 1993. Induction of glucose oxidase, catalase and lactonase in Aspergillus niger. Curr. Genet. 24:408-416.