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note
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For β-galactosidase staining, embryos were stained for lacZ activity as described (10). For Fig. 3, salmongal (Biosynth International, Skokie, IL.) was substituted for X-Gal. Embryos were fixed in 4% paraformaldehyde and processed for whole-mount in situ hybridization (11).
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note
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We thank D. Duboule, B. Hogan, and S.-J. Lee for plasmids and J. Gerhart, J. Ngai, and B. Skarnes for comments on the manuscript. Work in A.P.M.'s laboratory was supported by grants from the American Cancer Society (DB 88) and the NIH. Work in R.M.H.'s laboratory was supported by NIH grant GM49346. All animal care and experiments were in accordance with Harvard University and University of California at Berkeley Animal Care and Use Committee guidelines.
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