Establishing the Cryptosporidium parvum karyotype by NotI and SfiI restriction analysis and Southern hybridization

Gene

Volumn 219, Issue 1-2, 1998, Pages 73-79

  Cacciò, Simone ^a   Camilli, Romina ^a   La Rosa, Giuseppe ^a   Pozio, Edoardo ^a  

^a Laboratory of Parasitology   (Italy)

Author keywords

Chromosome; Gene mapping; Genome; Protozoan parasite

Indexed keywords

ALPHA TUBULIN; BETA TUBULIN; RESTRICTION ENDONUCLEASE; RIBOSOME DNA;

ARTICLE; BASE PAIRING; CHROMOSOMAL LOCALIZATION; CHROMOSOME ANALYSIS; CRYPTOSPORIDIUM PARVUM; DNA SEQUENCE; GENE LOCATION; GENE MAPPING; KARYOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; RESTRICTION MAPPING; SOUTHERN BLOTTING; SPECIES DIFFERENCE;

ANIMALS; CATTLE; CHROMOSOMES; CRYPTOSPORIDIUM PARVUM; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA PROBES; ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, GEL, PULSED-FIELD; KARYOTYPING; PROTOZOAN PROTEINS; RESTRICTION MAPPING;

APICOMPLEXA; COCCIDIA; CRYPTOSPORIDIUM PARVUM; PROTOZOA;

EID: 0032575914     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(98)00376-X     Document Type: Article

References (36)

1. Adam R.D., Nash T.K., Wellems T.E. The Giardia lamblia trophozoite contains sets of closely related chromosomes. Nucleic Acids Res. 16:1988;4555-4567.
2. Arrowood M.J., Sterling C.R. Isolation of Cryptosporidium parvum oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J. Parasitol. 73:1987;314-319.
3. Blunt D.S., Khramtsov N.V., Upton S.J., Montelone B.A. Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosome and a low-molecular size molecule. Clin. Diagn. Lab. Immunol. 4:1997;11-13.
4. Bonafonte M., Priest J.W., Garmon D., Arrowood M.J., Mead J.R. Isolation of the gene coding for elongation factor-1a in Cryptosporidium parvum. Biochem. Biophys. Acta. 1351:1997;256-260.
5. Bonnin A., Formaux M.N., Dubremetz J.F.et al. Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence. FEMS Microbiol. Lett. 137:1996;207-211.
6. Cacciò S., La Rosa G., Pozio E. The β-tubulin gene of Cryptosporidium parvum. Mol. Biochem. Parasitol. 89:1997;307-311.
7. Carraway M., Tzipori S., Widmer G. A new restriction fragment length polymorphism from Cryptosporidium parvum identifies genetically heterogeneous parasite populations and genotypic changes following transmission from bovine to human hosts. Infect. Immun. 65:1997;3958-3960.
8. Darlympe B.P. Cloning and characterization of the rRNA genes and flanking regions from Babesia bovis: use of the genes as strain discriminating probes. Mol. Biochem. Parasitol. 43:1990;117-124.
9. Foote S., Kemp D.J. Chromosomes of malaria parasites. Trends Genet. 5:1989;337-342.
10. Giannini S.H., Schittini M., Keithly J.S., Warburton P., Cantor C.R., Van der Ploeg L.H.T. Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis. Science. 232:1986;762-765.
11. Guay J., Huot A., Gagnon S., Tremblay A., Levesque R.C. Physical and genetic mapping of cloned ribosomal DNA from Toxoplasma gondii: primary and secondary structure of the 5S gene. Gene. 114:1992;165-171.
12. Hays M.P., Mosier D.A., Oberst R.D. Enhanced karyotype resolution of Cryptosporidium parvum by contour-clamped homogeneous electric fields. Vet. Parasitol. 58:1995;273-280.
13. Holloway S.P., Gerousis M., Delves C.J., Sims P.F.G., Scaife J.G., Hyde J.E. The tubulin genes of the human malaria parasite Plasmodium falciparum, their chromosomal location and sequence analysis of the α-tubulin II gene. Mol. Biochem. Parasitol. 43:1990;257-270.
14. Jenkins M.C., Fayer R. Cloning and expression of cDNA encoding an antigenic Cryptosporidium parvum protein. Mol. Biochem. Parasitol. 71:1995;149-152.
15. Jones D.E., Tu T.D., Mathur S., Sweeney R.W., Clark D.P. Molecular cloning and characterization of a Cryptosporidium parvum elongation factor-2 gene. Mol. Biochem. Parasitol. 71:1995;143-147.
16. Jones S.H., Lew A.E., Jorgensen W.K., Barker S.C. Babesia bovis: genome size, number of chromosomes and telomeric probe hybridization. Int. J. Parasitol. 27:1997;1569-1573.
17. Khramtsov N.V., Tilley M., Blunt D.S., Montelone B.A., Upton S.J. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form Hsp70. J. Eukaryot. Microbiol. 42:1995;416-422.
18. Khramtsov N.V., Blunt D.S., Montelone B.A., Upton S.J. The putative acetyl-CoA synthethase gene of Cryptosporidium parvum and a new conserved protein motif in acetyl-CoA synthethases. J. Parasitol. 82:1996;423-427.
19. Kibe M.K., ole-MoiYoi O., Nene V.et al. Evidence for two single-copy units in Theileria parva ribosomal RNA genes. Mol. Biochem. Parasitol. 66:1994;249-259.
20. Kim K., Goozé L., Petersen C., Gut J., Nelson R.G. Isolation, sequence and molecular karyotype analysis of the actin gene of Cryptosporidium parvum. Mol. Biochem. Parasitol. 50:1992;105-114.
21. Lai E., Birren B.W., Clark S.M., Simon M.I., Hood L. Pulsed field gel electrophoresis. Biotechniques. 7:1989;34-42.
22. Laxer M.A., Timblin B.K., Patel R.J. DNA sequences for the specific detection of Cryptosporidium parvum by the polymerase chain reaction. Am. J. Trop. Med. Hyg. 45:1991;688-694.
23. Le Blancq S.M., Khramtsov N.V., Zamani F., Upton S.J., Wu T.W. Ribosomal RNA gene organization in Cryptosporidium parvum. Mol. Biochem. Parasitol. 90:1997;463-478.
24. McCutchan T.F., Li J., McConkey G.A., Rogers M.J., Waters A.P. The cytoplasmic ribosomal RNAs of Plasmodium spp. Parasitol. Today. 11:1995;134-138.
25. Mead J.R., Arrowood M.J., Current W.L., Sterling C.R. Field inversion gel electrophoretic separation of Cryptosporidium spp. chromosome-sized DNA. J. Parasitol. 74:1988;366-369.
26. Morzaria S.P., Young J.R. Restriction mapping of the genome of the protozoan parasite Theileria parva. Proc. Natl. Acad. Sci. 89:1992;5241-5245.
27. Perkins M.E., Volkman S., Wirth D.F., Le Blancq S.M. Characterization of an ATP-binding cassette transporter in Cryptosporidium parvum. Mol. Biochem. Parasitol. 87:1997;117-122.
28. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning. A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Sheppard M., Thompson J.K., Anders R.F., Kemp D.J., Lew A.M. Molecular karyotyping of the rodent malarias Plasmodium chabaudi, Plasmodium berghei and Plasmodium vinckei. Mol. Biochem. Parasitol. 34:1989;45-52.
30. Shirley M.W. The genome of Eimeria tenella further studies on its molecular organisation. Parasitol. Res. 80:1994;366-373.
31. Sibley L.D., Boothroyd J.C. Construction of a molecular karyotype for Toxoplasma gondii. Mol. Biochem. Parasitol. 51:1992;291-300.
32. Spano F., Puri C., Ranucci L., Putignani L., Crisanti A. Cloning of the entire COWP gene of Cryptosporidium parvum and ultrastructural localization of the protein during sexual development. Parasitology. 114:1997;427-437.
33. Steele M.I., Kuhls T.L., Nida K.et al. A Cryptosporidium parvum genomic region encoding hemolytic activity. Infect. Immun. 63:1995;3840-3845.
34. Van der Ploeg L.H.T., Schwartz D.C., Cantor C.R., Borst P. Antigenic variation in Trypanosoma brucei analyzed by electrophoretic separation of chromosome-sized DNA molecules. Cell. 37:1984;77-84.
35. Vasquez J.R., Goozé L., Kim K., Gut J., Petersen C., Nelson R.G. Potential antifolate resistance determinants and genotypic variation in the bifunctional dihydrofolate reductase-thymidylate synthase gene from human and bovine isolates of Cryptosporidium parvum. Mol. Biochem. Parasitol. 79:1996;153-165.
36. Zhu G., Keithly J.S. Molecular analysis of a P-type ATPase from Cryptosporidium parvum. Mol. Biochem. Parasitol. 90:1997;307-316.