Potential antifolate resistance determinants and genotypic variation in the bifunctional dihydrofolate reductase-thymidylate synthase gene from human and bovine isolates of Cryptosporidium parvum

Molecular and Biochemical Parasitology

Volumn 79, Issue 2, 1996, Pages 153-165

  Vásquez, John R ^a   Goozé, Lisa ^a   Kim, Kami ^a,b   Gut, Jiří ^a   Petersen, Carolyn ^a   Nelson, Richard G ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Antifolate resistance; Cryptosporidium parvum; Dihydrofolate reductase; Genotypic variation; Intraspecific variation; Thymidylate synthase

Indexed keywords

ANTIPROTOZOAL AGENT; DIHYDROFOLATE REDUCTASE; FOLIC ACID ANTAGONIST; PROTOZOAL DNA; THYMIDYLATE SYNTHASE;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; AMINO ACID SEQUENCE; ARTICLE; CATTLE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; DNA POLYMORPHISM; DRUG RESISTANCE; GENOTYPE; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL;

BOS TAURUS; BOVINAE; CRYPTOSPORIDIUM PARVUM;

EID: 0030221884     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-6851(96)02647-3     Document Type: Article

References (41)

1. [1] O'Donoghue, P.J. (1995) Cryptosporidium and cryptosporidiosis in man and animals. Int. J. Parasitol. 25, 139-195.
2. [2] Anonymous (1982) Cryptosporidiosis - Alabama. Morb. Mort. Wkly. Rep. 31, 252-254.
3. [3] Anonymous (1982) Cryptosporidiosis: Assessment of chemotherapy of males with Acquired Immune Deficiency Syndrome (AIDS). Morb. Mort. Wkly. Rep. 31, 589-592.
4. [4] Anonymous (1984) Update: Treatment of cryptosporidiosis in patients with Acquired Immunodeficiency Syndrome (AIDS). Morb. Mort. Wkly. Rep. 33, 117-119.
5. [5] Ungar, B.L.P. (1995) 262. Cryptosporidium. In: Mandell, Douglas and Bennett's Principles and Practice of Infectious Diseases, Vol. 2, 4th edn. (Mandell, G.L., Bennett, J.E. and Dalin, R., eds.), pp. 2500-2510. Churchill Livingstone, New York.
6. [6] Kim, K., Goozé, L., Petersen, C., Gut, J. and Nelson, R.G. (1992) Isolation, sequence and molecular karyotype analysis of the actin gene of Cryptosporidium parvum. Mol. Biochem. Parasitol. 50, 105-113.
7. [7] Goozé, L., Kim, K., Petersen, C., Gut, J. and Nelson, R.G. (1991) Amplification of a Cryptosporidium parvum gene fragment encoding thymidylate synthase. J. Protozool. 38, 56S-58S.
8. [8] Nelson, R.G., Kim, K., Gooze, L., Petersen, C. and Gut, J. (1991) Identification and isolation of Cryptosporidium parvum genes encoding microtubule and microfilament proteins. J. Protozool. 38, 52S-55S.
9. [9] Petersen, C., Gut, J., Leech, J.H. and Nelson, R.G. (1992) Identification and initial characterization of five Cryptosporidium parvum sporozoite antigen genes. Infect. Immun. 60, 2343-2348.
10. [10] Chu, G., Vollrath, D. and Davis, R.W. (1986) Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science 234, 1582-1585.
11. [11] Genetics Computer Group (1994) Program Manual for the Wisconsin Package, Version 8, 575 Science Drive, Madison, Wisconsin, USA 53711.
12. [12] Oefner, C., D'Arcy, A. and Winkler, F.K. (1988) Crystal structure of human dihydrofolate reductase complexed with folate. Eur. J. Biochem. 174, 377-385.
13. [13] Bolin, J.T., Filman, D.J., Matthews, D.A., Hamlin, R.C. and Kraut, J. (1982) Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate. J. Biol. Chem. 257, 13650-13662.
14. [14] Filman, D.J., Bolin, J.T., Matthews, D.A. and Kraut, J. (1982) Crystal structures of Escherichia coli and Lacto bacillus casei dihydrofolate reductase refined at 1.7 A resolution. II. Environment of bound NADPH and implications for catalysis. J. Biol. Chem. 257, 13663-13672.
15. [15] Matthews, D.A., Bolin, J.T., Burridge, J.M., Filman, D.J., Volz, K.W., Kaufman, B.T., Beddell, C.R., Champness, J.N., Stammers, D.K. and Kraut, J. (1985) Refined crystal structures of Escherichia coli and chicken liver dihydrofolate reductase containing bound trimethoprim. J. Biol. Chem. 260, 381-391.
16. [16] Hardy, L.W., Finer-Moore, J.S., Montfort, W.R., Jones, M.O., Santi, D.V. and Stroud, R.M. (1987) Atomic structure of thymidylate synthase: target for rational drug design. Science 235, 448-455.
17. [17] Mortimer, R.K., Schild, D., Contopoulou, C.R. and Kans, J.A. (1991) Genetic and physical maps of Saccharomyces cerevisiae. Methods Enzymol. 194, 827-863.
18. [18] Barclay, B.J., Huang, T., Nagel, M.G., Misener, V.L., Game, J.C. and Wahl, G.M. (1988) Mapping and sequencing of the dihydrofolate reductase gene (DFR1) of Saccharomyces cerevisiae. Gene 63, 175-180.
19. [19] Matthews, D.A., Appelt, K., Oatley, S.J. and Xuong, N.H. (1990) Crystal structure of Escherichia coli thymidylate synthase containing bound 5-fluoro-2′-deoxyuridylate and 10-propargyl-5,8-dideazafolate. J. Mol. Biol. 214, 923-936.
20. [20] Bairoch, A. (1992) PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Res. 20, 2013-2018.
21. [21] Stringer, J.R. and Members of the Pneumocyctis Workshop (1994) Revised Nomenclature for Pneumocystis carinii. J. Euk. Microbiol. 41, 121S-122S.
22. [22] Mazars, E., Ödberg-Ferraagut, C., Del-Cas, E., Fourmaux, M.-N., Aliouat, E.M., Brun-Pascaud, M., Mougeot, G. and Camus, D. (1995) Polymorphism of the thymidylate synthase gene of Pneumocystis carinii from different host species. J. Euk. Microbiol. 42, 26-32.
23. [23] Basco, L.K., Depecoulas, P.E., Wilson, C.M., Lebras, J. and Mazabraud, A. (1995) Point mutations in the dihydrofolate reductase thymidylate synthase gene and pyrimethamine and cycloguanil resistance in Plasmodium falciparum. Mol. Biochem. Parasitol. 69, 135-138.
24. [24] Tanaka, M., Gu, H.M., Bzik, D.J., Li, W.B. and Inselburg, J.W. (1990) Dihydrofolate reductase mutations and chromosomal changes associated with pyrimethamine resistance of Plasmodium falciparum. Mol. Biochem. Parasitol. 39, 127-134.
25. [25] Cowman, A.F., Morry, M.J., Biggs, B.A., Cross, G.A.M. and Foote, S.J. (1988) Amino acid changes linked to pyrimethamine resistance in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum. Proc. Natl. Acad. Sci. USA 85, 9109-9113.
26. [26] Peterson, D.S., Milhous, W.K. and Wellems, T.E. (1990) Molecular basis of differential resistance to cycloguanil and pyrimethamine in Plasmodium falciparum malaria. Proc. Natl. Acad. Sci. USA 87, 3018-3022.
27. [27] Foote, S.J., Galatis, D. and Cowman, A.F. (1990) Amino acids in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum involved in cycloguanil resistance differ from those involved in pyrimethamine resistance. Proc. Natl. Acad. Sci. USA 87, 3014-3017.
28. [28] Ortega, Y.R., Sheehy, R.R., Cama, V.A., Oishi, K.K. and Sterling, C.R. (1991) Restriction fragment length polymorphism analysis of Cryptosporidium parvum isolates of bovine and human origin. J. Protozool. 38, 40S-41S.
29. [29] Awad-el-Kariem, F.M., Robinson, H.A., Dyson, D.A., Evans, D., Wright, S., Fox, M.T. and McDonald, V. (1995) Differentiation between human and animal strains of Cryptosporidium parvum using isoenzyme typing. Parasitology 110, 129-132.
30. [30] Morgan, U.M., Constantine, C.C., Odonoghue, P., Meloni, B.P., Obrien, P.A. and Thompson, R.C.A. (1995) Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis. Am. J. Trop. Med. Hyg. 52, 559-564.
31. [31] Carraway, M., Tzipori, S. and Widmer, G. (1996) Identification of genetic heterogeneity in the Cryptospridium parvum ribosomal repeat. Appl. Environ. Microbiol. 62, 712-716.
32. [32] Bonnin, A., Fourmaus, M.N., Dubremetz, J.F., Nelson, R.G., Gobet, P., Harly, G.M.B., Pinon, J.M., Gabriel-Pospisl, F., Naciri, M. and Camerlynck, P. (1996) Geno-typing human and bovine isolates of Cryptosporidium parvum by PCR-RFLP analysis of a repeated DNA sequence. FEMS Microbiol. Lett. 137. 207-211.
33. [33] Peterson, D.S., Walliker, D. and Wellems, T.E. (1988) Evidence that a point mutation in dihydrofolate reductase-thymidylate synthase confers resistance to pyrimethamine in falciparum malaria. Proc. Natl. Acad. Sci. USA 85, 9114-9118.
34. [34] Sirawaraporn, W., Sirawaraporn, R., Cowman, A.F., Yuthavong, Y. and Santi, D.V. (1990) Heterologous expression of active thymidylate synthase-dihydrofolate reductase from Plasmodium falciparum. Biochemistry 29, 10779-10785.
35. [35] Donald, R.G.K. and Roos, D.S. (1993) Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistant mutations in malaria. Proc. Natl. Acad. Sci. USA 90, 11703-11707.
36. [36] Wu, Y., Kirkman, L.A. and Wellems, T.E. (1996) Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proc. Natl. Acad. Sci. USA 93, 1130-1134.
37. [37] van Dijk, M.R., Waters, A.P. and Janse, C.J. (1995) Stable transfection of malaria parasite blood stages. Science 268, 1358-1362.
38. [38] Zindrou, S., Dao, L.-D., Thi Xuyen, P., Phuong Dung, N., Duy Sy, N., Skold, O. and Swedberg, G. (1996) Rapid detection of pyrimethamine susceptibility of Plasmodium falciparum by restriction endonuclease digestion of the dihydrofolate reductase gene. Am. J. Trop. Med. Hyg. 54, 185-188.
39. [39] Thaithong, S., Chan, S.W., Songsomboon, S., Wilairat, P., Seesod, N., Sueblinwong, T., Goman, M., Ridley, R. and Beale, G. (1992) Pyrimethamine resistant mutations in Plasmodium falciparum. Mol. Biochem. Parasitol. 52, 149-157.
40. [40] Thillet, J., Absil, J., Stone, S.R. and Pictet, R. (1988) Site-directed mutagenesis of mouse dihydrofolate reductase. Mutants with increased resistance to methotrexate and trimethoprim. J. Biol. Chem. 263, 12500-12508.
41. [41] Champness, J.N., Achari, A., Ballantine, S.P., Bryant, P.K., Delves, C.J. and Stammers, D.K. (1994) The structure of Pneumocystis carinii dihydrofolate reductase to 1.9Å resolution. Structure 2, 915-924.