The axial tyrosinate Fe3+ ligand in protocatechuate 3,4-dioxygenase influences substrate binding and product release: Evidence for new reaction cycle intermediates

Biochemistry

Volumn 37, Issue 8, 1998, Pages 2131-2144

  Frazee, Richard W ^a,b   Orville, Allen M ^a,c   Dolbeare, Kevin B ^a   Yu, Hong ^a   Ohlendorf, Douglas H ^a   Lipscomb, John D ^a,d  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MICHIGAN FLINT   (United States)

^c UNIVERSITY OF OREGON   (United States)

^d UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FERRIC ION; PROTOCATECHUATE 3,4 DIOXYGENASE; TYROSINE; IRON; LIGAND; OLIGODEOXYRIBONUCLEOTIDE; RECOMBINANT PROTEIN;

ARTICLE; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME SUBSTRATE; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; SITE DIRECTED MUTAGENESIS; BINDING SITE; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; ELECTROCHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POINT MUTATION; PROTEIN CONFORMATION; PROTEUS MIRABILIS;

BASE SEQUENCE; BINDING SITES; CLONING, MOLECULAR; ELECTROCHEMISTRY; IRON; KINETICS; LIGANDS; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; POINT MUTATION; PROTEIN CONFORMATION; PROTEUS MIRABILIS; PROTOCATECHUATE-3,4-DIOXYGENASE; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TYROSINE;

EID: 0032562027     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi972047b     Document Type: Article

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