Requirements for Γδ T cells in allergic airway inflammation

Science

Volumn 280, Issue 5367, 1998, Pages 1265-1267

  Zuany Amorim, Claudia ^a,b   Ruffié, Claude ^a   Hailé, Solomon ^a   Vargaftig, B Boris ^a   Pereira, Pablo ^a   Pretolani, Marina ^a  

^a INSTITUT PASTEUR   (France)

^b NOVARTIS HORSHAM RESEARCH CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALLERGIC ASTHMA; ANIMAL CELL; ANTIBODY RESPONSE; ANTIGEN RECOGNITION; ARTICLE; BRONCHUS REACTIVITY; HELPER CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; ANIMAL; ASTHMA; BAGG ALBINO MOUSE; BIOSYNTHESIS; BLOOD; BRONCHUS; BRONCHUS HYPERREACTIVITY; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CROSS BREEDING; EOSINOPHIL; FEMALE; IMMUNIZATION; IMMUNOLOGY; LUNG; LUNG LAVAGE; MALE; T LYMPHOCYTE SUBPOPULATION; TH2 CELL;

GAMMA INTERFERON; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; INTERLEUKIN 4; INTERLEUKIN 5; LYMPHOCYTE ANTIGEN RECEPTOR; OVALBUMIN;

ANIMALS; ASTHMA; BRONCHI; BRONCHIAL HYPERREACTIVITY; BRONCHOALVEOLAR LAVAGE FLUID; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CROSSES, GENETIC; EOSINOPHILS; FEMALE; IMMUNIZATION; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; INTERFERON TYPE II; INTERLEUKIN-4; INTERLEUKIN-5; LUNG; MALE; MICE; MICE, INBRED BALB C; OVALBUMIN; RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA; T-LYMPHOCYTE SUBSETS; TH2 CELLS;

EID: 0032557246     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5367.1265     Document Type: Article

References (36)

1. B. Burrows, F. D. Martinez, M. Halonen, R. A. Barbee, M. G. Cline, N. Engl. J. Med. 320, 271 (1989); J. Bousquet et al., ibid. 323, 1033 (1990); D. S. Robinson et al., ibid. 326, 298 (1992).
2. B. Burrows, F. D. Martinez, M. Halonen, R. A. Barbee, M. G. Cline, N. Engl. J. Med. 320, 271 (1989); J. Bousquet et al., ibid. 323, 1033 (1990); D. S. Robinson et al., ibid. 326, 298 (1992).
3. B. Burrows, F. D. Martinez, M. Halonen, R. A. Barbee, M. G. Cline, N. Engl. J. Med. 320, 271 (1989); J. Bousquet et al., ibid. 323, 1033 (1990); D. S. Robinson et al., ibid. 326, 298 (1992).
4. R. I. Tepper et al., Cell 62, 457 (1990); M. Kopf, G. Le Gros, M. Bachmann, Nature 362, 245 (1993); M. Vajdy, M. H. Kosco-Vilbois, M. Kopf, G. Kölher, N. Lycke, J. Exp. Med. 181, 41 (1995); J. A. Rankin et al., Proc. Natl. Acad. Sci. U.S.A. 93, 7821 (1996).
5. R. I. Tepper et al., Cell 62, 457 (1990); M. Kopf, G. Le Gros, M. Bachmann, Nature 362, 245 (1993); M. Vajdy, M. H. Kosco-Vilbois, M. Kopf, G. Kölher, N. Lycke, J. Exp. Med. 181, 41 (1995); J. A. Rankin et al., Proc. Natl. Acad. Sci. U.S.A. 93, 7821 (1996).
6. R. I. Tepper et al., Cell 62, 457 (1990); M. Kopf, G. Le Gros, M. Bachmann, Nature 362, 245 (1993); M. Vajdy, M. H. Kosco-Vilbois, M. Kopf, G. Kölher, N. Lycke, J. Exp. Med. 181, 41 (1995); J. A. Rankin et al., Proc. Natl. Acad. Sci. U.S.A. 93, 7821 (1996).
7. R. I. Tepper et al., Cell 62, 457 (1990); M. Kopf, G. Le Gros, M. Bachmann, Nature 362, 245 (1993); M. Vajdy, M. H. Kosco-Vilbois, M. Kopf, G. Kölher, N. Lycke, J. Exp. Med. 181, 41 (1995); J. A. Rankin et al., Proc. Natl. Acad. Sci. U.S.A. 93, 7821 (1996).
8. D. A. Ferrick et al., Nature 373, 255 (1995).
9. A. P. Vicari, S. Mocci, P. Openshaw, A. O'Garra, A. Zlotnik, Eur. J. Immunol. 26, 1424 (1996); V. Azuara, J. P. Levraud, M. P. Lembezat, P. Pereira, ibid. 27, 544 (1997).
10. A. P. Vicari, S. Mocci, P. Openshaw, A. O'Garra, A. Zlotnik, Eur. J. Immunol. 26, 1424 (1996); V. Azuara, J. P. Levraud, M. P. Lembezat, P. Pereira, ibid. 27, 544 (1997).
11. S. Itohara et al., Cell 72, 337 (1993).
12. All mice were immunized intraperitoneally with 10 μg of OVA in 0.1 ml of sterile saline every 2 days for 2 weeks [D. I. Blyth, M. S. Pedrick, T. J. Savage, E. M. Hessel, D. Fattah, Am. J. Respir. Cell Mol. Biol. 14, 425 (1996); Y. Chvatchko, M. H. Kosko-Vilbois, S. Herren, J. Lefort, J. Y. Bonnefoy, J. Exp. Med. 184, 2353 (1996)]. Forty days after the beginning of immunization, mice were challenged three times, each 3 days apart, with 20 μg of OVA in 50 μl of saline delivered intranasally. Control animals were similarly immunized intraperitoneally with OVA and challenged intranasally three times with 50 μl of saline. Mice were used 3 days after the last intranasal saline or OVA challenge.
13. All mice were immunized intraperitoneally with 10 μg of OVA in 0.1 ml of sterile saline every 2 days for 2 weeks [D. I. Blyth, M. S. Pedrick, T. J. Savage, E. M. Hessel, D. Fattah, Am. J. Respir. Cell Mol. Biol. 14, 425 (1996); Y. Chvatchko, M. H. Kosko-Vilbois, S. Herren, J. Lefort, J. Y. Bonnefoy, J. Exp. Med. 184, 2353 (1996)]. Forty days after the beginning of immunization, mice were challenged three times, each 3 days apart, with 20 μg of OVA in 50 μl of saline delivered intranasally. Control animals were similarly immunized intraperitoneally with OVA and challenged intranasally three times with 50 μl of saline. Mice were used 3 days after the last intranasal saline or OVA challenge.
14. Seventy-two hours after the final intranasal saline or antigen challenge, animals were sacrificed and lungs were inflated by injecting into the trachea a 1-ml solution of optimum cutter temperature compound (OCT; BDH, Poole, UK) in distilled water (1:1). The dissected lobes were covered by OCT, placed in Eppendorf vials, frozen in liquid nitrogen, and kept at -80°C until use. Six-micrometer sections alongside the main intrapulmonary bronchus were cut in a cryostat and collected on glass slides previously coated with γ-methacryloxy propyltrimethoxysilane (Sigma) and fixed in acetone for 10 min. Cyanide-resistant eosinophil peroxidase activity, using potassium cyanide, diaminobenzidine, and hydrogen peroxide, was used to stain the eosinophils [C. Zuany-Amorim et al., J. Clin. Invest. 95, 2644 (1995)]. For the anti-CD4 and anti-CD8 antibodies (Tebu; Le Perray-en-Yvelines, France), an alkaline phosphatase-anti-alkaline phosphatase staining procedure was performed. Positive cells were enumerated in 1 μm of bronchial mucosa and results were expressed as numbers of cells per millimeter of bronchial epithelium.
15. IL-4, IL-5, and IFN-γ were measured in the supernatant of BAL fluids by either specific enzyme-immunometric assay (IL-5) [C. Zuany-Amorim et al., J. Immunol. 157, 377, 1996] or enzyme-linked immunosorbent assay (ELISA) (IL-4 and IFN-γ). Sensitivities were 50 pg/ml (IL-4), 5 pg/ml (IL-5), and 50 units/ml (IFN-γ). Recombinant murine IL-4, IL-5 (Immugenex; Los Angeles, CA), and IFN-γ (Sigma) were used to generate standard curves.
16. Unrestrained conscious mice were placed in a whole-body plethysmographic chamber (BUXCO Electronics; Sharon, CT), which analyzed the respiratory wave forms. After stabilization for a few minutes, an aerosol of methacholine (30 mM in the aerosolator) was delivered for 20 s. The airways resistance was expressed as Penh = 0.67 × [(expiratory time/40% of relaxation time) - 1] (peak expiratory flow/peak inspiratory flow) [S. Y. Eum, S. Hailé, J. Lefort, M. Huerre, B. B. Vargaftig, Proc. Natl. Acad. Sci. U.S.A., 92, 12290 (1995)].
17. OVA-specific IgE and IgG1 in the serum samples were measured by ELISA, using biotinylated rat IgE antibody to mouse (Pharmingen; San Diego, CA) and alkaline phosphatase-conjugated goat IgG1 antibody to mouse (Southern Biotechnology; Birmingham, AL). To detect OVA-specific IgE, 25 μl of each serum was incubated twice with 50 μl of a 50% slurry of protein G-Sepharose (Pharmacia) in phosphate-buffered saline before the ELISA. This treatment allows the removal of about 95% of total IgG1 without altering the concentration of IgE.
18. Mice were anesthetized by an intraperitoneal injection of 1.4 g of ethylcarbamate per kilogram of body weight and BAL cells were harvested by injecting and recovering 1.5 ml of sterile saline through a tracheal cannula. Blood samples were also collected from the tail vein and total leukocytes and BAL cells were counted. Peripheral and BAL eosinophils were identified and counted after cytocentrifugation and staining with Diff-Quik stain (Baxter Dade; Duedingen, Switzerland). Bone marrow cells were collected from femurs in RPMI 1640 medium supplemented with 10% fetal calf serum, and eosinophils were enumerated after cytocentrifugation and peroxidase staining (7).
19. -/- mice, respectively (n = 10, P = 0.001).
20. -/- mice, respectively (P = 0.018, n = 17).
21. D. B. Corry et al., J. Exp. Med. 183, 1 (1996); W. R. Henderson Jr. et al., ibid. 184, 1483 (1996).
22. D. B. Corry et al., J. Exp. Med. 183, 1 (1996); W. R. Henderson Jr. et al., ibid. 184, 1483 (1996).
23. P. Pereira et al., data not shown.
24. 1 mice (5 to 12 mice per group) were immunized and challenged with OVA (6). No significant differences in the numbers of eosinophils (11) and in the release of IL-5 (8) in the BAL fluid, as well as in OVA-specific IgE and IgG1 serum titers (70), were noted among these strains.
25. G.C. Brusselle et al., Clin. Exp. Allergy 24, 73 (1994); A. J. Coyle et al., Am. J. Respir. Cell Mol. Biol. 13, 54 (1995); C. Zuany-Amorim et al., data not shown.
26. G.C. Brusselle et al., Clin. Exp. Allergy 24, 73 (1994); A. J. Coyle et al., Am. J. Respir. Cell Mol. Biol. 13, 54 (1995); C. Zuany-Amorim et al., data not shown.
27. G.C. Brusselle et al., Clin. Exp. Allergy 24, 73 (1994); A. J. Coyle et al., Am. J. Respir. Cell Mol. Biol. 13, 54 (1995); C. Zuany-Amorim et al., data not shown.
28. F. D. Finkelman et al., J. Immunol. 151, 1235 (1993).
29. A. Wodnar-Filipowicz, C. H. Heusser, C. Moroni, Nature 339, 150 (1989); F. Erard, M. T. Wild, J. A. Garcia-Sanz, G. Le Gros, Science 260, 1802 (1993); H. Arase, N. Arase, K. Nakagawa, R. A. Good, K. Onoe, Eur. J. Immunol. 23, 307 (1993).
30. A. Wodnar-Filipowicz, C. H. Heusser, C. Moroni, Nature 339, 150 (1989); F. Erard, M. T. Wild, J. A. Garcia-Sanz, G. Le Gros, Science 260, 1802 (1993); H. Arase, N. Arase, K. Nakagawa, R. A. Good, K. Onoe, Eur. J. Immunol. 23, 307 (1993).
31. A. Wodnar-Filipowicz, C. H. Heusser, C. Moroni, Nature 339, 150 (1989); F. Erard, M. T. Wild, J. A. Garcia-Sanz, G. Le Gros, Science 260, 1802 (1993); H. Arase, N. Arase, K. Nakagawa, R. A. Good, K. Onoe, Eur. J. Immunol. 23, 307 (1993).
32. T. T. Kung et al., Am. J. Respir. Cell Mol. Biol. 12, 404 (1995).
33. D. R. Brown et al., J. Exp. Med. 184, 1295 (1996); Y. Zhang, K. H. Rogers, D. B. Lewis, ibid., p. 1507.
34. D. R. Brown et al., J. Exp. Med. 184, 1295 (1996); Y. Zhang, K. H. Rogers, D. B. Lewis, ibid., p. 1507.
35. L. Wen et al., J. Exp. Med. 183, 2271 (1996).
36. -/- mice, E. Kilchherr and C. Heusser for the gift of IL-4, D. Joseph for eosinophil determinations in the bone marrow, G. Langsley for reading the manuscript, and J. M. Panaud for help in preparing photographic prints. Supported in part by grant 6969 from the Association pour la Recherche sur le Cancer.