Novel mutants of NAB corepressors enhance activation by Egr transactivators

EMBO Journal

Volumn 17, Issue 20, 1998, Pages 6010-6019

  Svaren, John ^a   Sevetson, Bradley R ^a   Golda, Thomas ^a,b   Stanton, Jeffrey J ^a   Swirnoff, Alex H ^a,c   Milbrandt, Jeffrey ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^c GENENTECH INC   (United States)

Author keywords

Corepressor; Dominant negative; Egr; FGF; NAB

Indexed keywords

CELL PROTEIN; MUTANT PROTEIN; PROTEIN NAB1; PROTEIN NAB2; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE REPRESSION; GENE TARGETING; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; TRANSACTIVATION; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; ANIMALS; CAENORHABDITIS ELEGANS; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; CONSERVED SEQUENCE; GENES, HELMINTH; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NEOPLASM PROTEINS; PEPTIDE FRAGMENTS; PRECIPITIN TESTS; PROTEIN BINDING; RATS; REPRESSOR PROTEINS; TRANS-ACTIVATORS;

EID: 0032531903     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.20.6010     Document Type: Article

References (45)

1. Biesiada, E., Razandi, M. and Levin, E.R, (1996) Egr-1 activates basic fibroblast growth factor transcription. Mechanistic implications for astrocyte proliferation. J. Biol. Chem., 271, 18576-18581.
2. Brachmann, R.K., Vidal, M. and Boeke, J.D. (1996) Dominant-negative p53 mutations selected in yeast hit cancer hot spots. Proc. Natl Acad. Sci. USA, 93, 4091-4095.
3. Christianson, T.W., Sikorski, R.S., Dante, M., Shero, J.H. and Hieter, P. (1992) Multifunctional yeast high-copy-number shuttle vectors. Gene, 110, 119-122.
4. Cui, M.Z., Parry, G.C, Oeth, P., Larson, H., Smith, M., Huang, R.P, Adamson, E.D. and Mackman, N. (1996) Transcriptional regulation of the tissue factor gene in human epithelial cells is mediated by Sp1 and EGR-1. J. Biol. Chem., 271, 2731-2739.
5. Day, M.L., Fahrner, T.J., Ayken, S. and Milbrandt, J. (1990) The zinc finger protein NGFI-A exists in both nuclear and cytoplasmic forms in nerve growth factor-stimulated PC12 cells. J. Biol. Chem., 265, 15253-15260.
6. Fan, W., Ma, J.X., Cheng, L. and Norris, J.S. (1997) Molecular cloning of TA 16, a transcriptional represser that may mediate glucocorticoid- induced growth arrest of leiomyosarcoma cells. Mol. Endocrinol., 11, 1342-1352.
7. Gashler, A. and Sukhatme, V.P. (1995) Early growth response protein 1 (Egr-1): prototype of a zinc-finger family of transcription factors. Prog. Nucleic Acid Res. Mol. Biol., 50, 191-224.
8. Harper, J.W., Adami, G.R., Wei, N., Keyomarsi, K. and Elledge, S. (1993) The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell, 75, 805-816.
9. Hassan, S.M, Kerkhoff, H., Troost, D., Veldman, H. and Jennekens, F.G. (1994) Basic fibroblast growth factor immunoreactivity in the peripheral motor system of the rat. Acta Neitropathol., 87, 405-410.
10. Kern, S.E., Pietenpol, J.A., ThiagalingamS., Seymour, A. Kinzler, K.W. and Vogelstein, B. (1992) Oncogenic forms of p53 inhibit p53-regulated gene expression. Science, 256, 827-830.
11. Khachigian, L.M., Williams, A.J. and Collins, J. (1995) Interplay of Sp1 and Egr-1 in the proximal platelet-derived growth factor A-chain promoter in cultured vascular endothelial cells. J. Biol. Chem., 270, 27679-27686.
12. Khachigian, L.M., Lindner, V., Wiliiams, A.J. and Collins, T. (1996) Egr- 1-induced endothelial gene expression: a common theme in vascular injury. Science, 271. 1427-1431.
13. Kim, S.J., Park, K., Rudkin, B.B., Dey, B.R., Sporn, M.B. and Roberts, A.B. (1994) Nerve growth factor induces transcription of transforming growth factor-β1 through a specific promoter element in PC12 cells. J. Biol. Chem., 269, 3739-3744.
14. Kirsch, K.H., Korradi, Y. and Johnson, J.P. (1996) Mader: a novel nuclear protein over expressed in human melanomas. Oncogene, 12, 963-971.
15. Lamb, T.M. and Harland, R.M. (1995) Fibroblast growth factor is a direct neural inducer, which combined with noggin generates anteriorposterior neural pattern. Development, 121, 3627-3636.
16. Lee, S.L., Sadovsky, Y., Swimoff, A.H., Polish, JA, Goda, P, Gavrilina, G. and Milbrandt, J. (1996) Luteinizing hormone deficiency and female infertility in mice lacking the transcription factor NGFI-A (Egr-1). Science, 273, 1219-1222.
17. Levi, G., Topilko, P., Schneider-Maunouiy, S., Lasagna, M., Mantero, S., Cancedda, R. and Charnay, P. (1996) Defective bone formation in Krox- 20 mutant mice. Development, 122, 113-120.
18. Li, B. and Fields, S. (1993) Identification of mutations in p53 that affect its binding to SV40 large T antigen by using the yeast two-hybrid system. FASEB J., 7, 957-963.
19. Liu, C., Adamson, E. and Mercola, D. (1996) Transcription factor EGR- 1 suppresses the growth and transformation of human HT-1080 fibrosarcoma cells by induction of transforming growth factor beta 1. Proc. Natl Acad. Sci. USA, 93, 11831-11836.
20. Liu, J., Wilson, T.E., Milbrandt, J. and Johnston, M. (1993) Identifying DNA-binding sites and analyzing DNA-binding domains using a yeast selection system. Methods: A Companion to Methods Enzymol., 5, 125-137.
21. Muhlrad, D., Hunter, R. and Parker.R. (1992) A rapid method for localized mutagenesis of yeast genes. Yeast, 8, 79-82.
22. Paulsen, R-E., Weaver, C.A., Fahrner, T.J. and Milbrandt, J. (1992) Domains regulating transcriptional activity of the inducible orphan receptor NGFI-B. J. Biol. Chew., 267, 16491-16496.
23. Rose, M.S., Winston, F, and Hieter, P. (1990) Methods in Yeast Genetics: A Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Russo, M.W., Matheny, C. and Milbrandt, J. (1993) Transcriptional activity of the zinc finger protein NGFI-A is influenced by its interaction with a cellular factor, Mol. Cell. Biol., 13, 6858-6865.
25. Russo, M.W., Sevetson, B.R. and Milbrandt, J. (1995) Identification of NAB1, a repressor of NGFI-A and Krox20 mediated transcription. Proc. Natl Acad. Sci. USA, 92, 6873-6877.
26. Sadowski, I. and Ptashne, M. (1989) A vector for expressing GAL4 (1- 147) fusions in mammalian cells. Nucleic Acids Res., 17, 7539.
27. Scherer, S.S. (1997) The biology and pathobiology of Schwann cells. Curr. Opin. Neurol., 10, 386-397.
28. Schneider-Maunoury, S., Topilko, P, Seitanidou, T., Levi, G., Cohen-Tannoudji, M., Pournin, S., Babinet, C. and Charnay, P. (1993) Disruption of Krox-20 results in alteration of rhombomeres 3 and 5 in the developing hindbrain. Cell, 75, 1199-1214.
29. Seitanidou, T., Schneider-Maunoury, S., Desmarquet, C.D.G.W. and Charnay, P. (1997) Krox-20 is a key regulator of rhombomere-specific gene expression in the developing hindbrain. Mech. Dev., 65, 31-42.
30. Sham, M.H. et al. (1993) The zinc finger gene Krox-20 regulates HoxB2 (Hox-2.8) during hindbrain segmentation. Cell, 72, 183-196.
31. Shan, B., Durfee, T. and Lee, W.H. (1996) Disruption of RB/E2F-1 interaction by single point mutations in E2F-1 enhances S-phase entry and apoptosis, Proc. Natl Acad. Sci. USA, 93, 679-684.
32. Shaulian, E., Zauberman, A., Ginsberg, D. and Oren, M. (1992) Identification of a minimal transforming domain of p53: negative dominance through abrogation of sequence-specific DNA binding. Mol. Cell. Biol., 12, 5581-5592.
33. Sikorski, R.S. and Boeke, J.D. (1991) In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant yeast. Methods Enzymol., 194, 302-318.
34. Svaren, J., Sevetson, B.R., Apel, E.D., Zimonjic, D.B., Popescu, N.C. and Milbrandt, J. (1996) NAB2, a corepressor of NGFI-A (Egr-1) and Krox 20, is induced by proliferative and differentiative stimuli. Mol. Cell. Biol., 16, 3545-3553.
35. Swiutek, P.J. and Gridley, T. (1993) Perinatal lethality and defects in hindbrain development in mice homozygous for a targeted mutation of the zinc finger gene Krox20. Genes Dev., 7, 2071-2084.
36. Swirnoff, A.H., Apel, E.D., Svaren, J., Sevetson, B.R., Zimonjic, D.B., Popescu, N.C. and Milbrandt, J. (1998) Nabl, a corepressor of NGFIA (Egr-1), contains an active transcriptional repression domain. Mol. Cell. Biol., 18, 512-524.
37. Theil, T., Frain, M., Gilardi-Hebenstreit, P., Flenniken, A.P.C. and Wilkinson, D.G. (1998) Segmental expression of the EphA4 (Sek-1) receptor tyrosine kinase in the hindbrain is under direct transcriptional control of Krox-20. Development, 125, 443-452.
38. Topilko, P., Schneider-Maunoury, S., Levi, G., Baron-Van Evercooren, A., Chennoufi, AB.Y., Seitanidou, T., Babinet, C. and Chamay, P. (1994) Krox-20 controls myelination in the peripheral nervous system. Nature, 371, 796-799.
39. Toumay, O. and Benezra, R. (1996) Transcription of the dominant-negative helix-loop-helix protein Idl is regulated by a protein complex containing the immediate-early response gene Egr-1 Mol. Cell. Biol., 16, 2418-2430.
40. Vidal, M., Brachmann, R.K., Fattaey, A., Harlow, E. and Boeke, J.D. (1996) Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. Proc. Natl Acad. Sci. USA, 93, 10315-10320.
41. Wang, D., Mayo, M.W. and Baldwin, A.S.J. (1997) Basic fibroblast growth factor transcriptional autoregulation requires EGR-1. Oncogens, 14, 2291-2299.
42. Warner, L.E., Mancias, P., Butler, I.J., McDonald, C.M., Keppen, L., Koob, K.G. and Lupski, J.R. (1998) Mutations in the early growth response 2 (EGR2) gene are associated with hereditary myelinopathies. Nature Genet., 18, 382-384.
43. White, M.A., Nicolette, C., Minden, A., Polverino, A., Van Aelst, L., Karin, M. and Wigler, M.H. (1995) Multiple ras functions can contribute to mammalian cell transformation. Cell, 80, 533-541.
44. Wilson, T.E., Fahrner, T.J., Johnston, M. and Milbrandt, J. (1991) Identification of the DNA binding site for NGFI-B by genetic selection in yeast. Science, 252, 1296-1300.
45. Wilson, T.E., Padgett, K.A., Johnston, M. and Milbrandt, J. (1993) A genetic method for defining DNA binding domains: application to the nuclear receptor NGFI-B. Proc. Natl Acad. Sci. USA, 90, 9186-9190.