메뉴 건너뛰기




Volumn 271, Issue 5254, 1996, Pages 1427-1431

Egr-1-induced endothelial gene expression: A common theme in vascular injury

Author keywords

[No Author keywords available]

Indexed keywords

PLATELET DERIVED GROWTH FACTOR;

EID: 0029996110     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5254.1427     Document Type: Article
Times cited : (485)

References (44)
  • 1
    • 0028966599 scopus 로고
    • M. A Gimbrone Jr, Am. J. Cardiol, 75, 67B (1995), E. Mattsson and A W Clowes, Trends Cardiovasc Med. 5, 200 (1995), R. Ross, Nature 362, 801 (1993).
    • (1995) Am. J. Cardiol , vol.75 B , pp. 67
    • Gimbrone Jr., M.A.1
  • 3
    • 0027241856 scopus 로고
    • M. A Gimbrone Jr, Am. J. Cardiol, 75, 67B (1995), E. Mattsson and A W Clowes, Trends Cardiovasc Med. 5, 200 (1995), R. Ross, Nature 362, 801 (1993).
    • (1993) Nature , vol.362 , pp. 801
    • Ross, R.1
  • 4
    • 0027982035 scopus 로고
    • M. D. Rekhter and D. Gordon, Circ. Res. 75, 410 (1994); E. G Nabel et al., J Clin. Invest. 91, 1822 (1993); A. Jawien, D. F. Bowen-Pope, V. Lindner, S. M. Schwartz, A. W. Clowes, ibid. 89, 507 (1992), G. A. A. Ferns et al., Science 263, 1129 (1991); J N. Wilcox, K M. Smith, L. T. Williams, S. M Schwartz, D. Gordon, J. Clin. Invest. 82, 1134 (1988).
    • (1994) Circ. Res. , vol.75 , pp. 410
    • Rekhter, M.D.1    Gordon, D.2
  • 5
    • 0027207192 scopus 로고
    • M. D. Rekhter and D. Gordon, Circ. Res. 75, 410 (1994); E. G Nabel et al., J Clin. Invest. 91, 1822 (1993); A. Jawien, D. F. Bowen-Pope, V. Lindner, S. M. Schwartz, A. W. Clowes, ibid. 89, 507 (1992), G. A. A. Ferns et al., Science 263, 1129 (1991); J N. Wilcox, K M. Smith, L. T. Williams, S. M Schwartz, D. Gordon, J. Clin. Invest. 82, 1134 (1988).
    • (1993) J Clin. Invest. , vol.91 , pp. 1822
    • Nabel, E.G.1
  • 6
    • 0026534274 scopus 로고
    • M. D. Rekhter and D. Gordon, Circ. Res. 75, 410 (1994); E. G Nabel et al., J Clin. Invest. 91, 1822 (1993); A. Jawien, D. F. Bowen-Pope, V. Lindner, S. M. Schwartz, A. W. Clowes, ibid. 89, 507 (1992), G. A. A. Ferns et al., Science 263, 1129 (1991); J N. Wilcox, K M. Smith, L. T. Williams, S. M Schwartz, D. Gordon, J. Clin. Invest. 82, 1134 (1988).
    • (1992) J Clin. Invest. , vol.89 , pp. 507
    • Jawien, A.1    Bowen-Pope, D.F.2    Lindner, V.3    Schwartz, S.M.4    Clowes, A.W.5
  • 7
    • 0026418308 scopus 로고
    • M. D. Rekhter and D. Gordon, Circ. Res. 75, 410 (1994); E. G Nabel et al., J Clin. Invest. 91, 1822 (1993); A. Jawien, D. F. Bowen-Pope, V. Lindner, S. M. Schwartz, A. W. Clowes, ibid. 89, 507 (1992), G. A. A. Ferns et al., Science 263, 1129 (1991); J N. Wilcox, K M. Smith, L. T. Williams, S. M Schwartz, D. Gordon, J. Clin. Invest. 82, 1134 (1988).
    • (1991) Science , vol.263 , pp. 1129
    • Ferns, G.A.A.1
  • 8
    • 0023694258 scopus 로고
    • M. D. Rekhter and D. Gordon, Circ. Res. 75, 410 (1994); E. G Nabel et al., J Clin. Invest. 91, 1822 (1993); A. Jawien, D. F. Bowen-Pope, V. Lindner, S. M. Schwartz, A. W. Clowes, ibid. 89, 507 (1992), G. A. A. Ferns et al., Science 263, 1129 (1991); J N. Wilcox, K M. Smith, L. T. Williams, S. M Schwartz, D. Gordon, J. Clin. Invest. 82, 1134 (1988).
    • (1988) J. Clin. Invest. , vol.82 , pp. 1134
    • Wilcox, J.N.1    Smith, K.M.2    Williams, L.T.3    Schwartz, S.M.4    Gordon, D.5
  • 18
    • 13344264830 scopus 로고    scopus 로고
    • note
    • Sequences of Oligo B and Oligo Bm are 5′-GCTGTCTCCACCCACCTCTCGCACTCT-3′ and 5′-GCTGTAGAAGAACAAGAGTACACTCT-3′, respectively.
  • 21
    • 0028304008 scopus 로고
    • B. R. Dey et al., Mol. Endocrinol. 8, 595 (1994), S. J Kim, A Glick, M. B. Sporn, A. B. Roberts, J Biol Chem. 264, 402 (1989).
    • (1994) Mol. Endocrinol. , vol.8 , pp. 595
    • Dey, B.R.1
  • 23
    • 0029100932 scopus 로고
    • N. Mackman, FASEB J 9, 883 (1995), M.-Z Cui, G C N. Parry, T. S Edgington, N. Mackman, Arterioscler. Thromb. 14, 807 (1994).
    • (1995) FASEB J , vol.9 , pp. 883
    • Mackman, N.1
  • 27
    • 13344288329 scopus 로고    scopus 로고
    • M. Z. Gui et al., ibid. 271, 2731 (1996); A. L. Gashler S. Swaminathan, V. P Sukhatme, Mol Cell. Biol. 13 4556 (1993); X-M Cao et al., ibid. 10, 1931 (1990)
    • (1996) J Biol. Chem. , vol.271 , pp. 2731
    • Gui, M.Z.1
  • 29
    • 0025317094 scopus 로고
    • M. Z. Gui et al., ibid. 271, 2731 (1996); A. L. Gashler S. Swaminathan, V. P Sukhatme, Mol Cell. Biol. 13 4556 (1993); X-M Cao et al., ibid. 10, 1931 (1990)
    • (1990) Mol Cell. Biol. , vol.10 , pp. 1931
    • Cao, X.-M.1
  • 30
    • 0028858061 scopus 로고
    • M. J. Reed et al., Am. J. Pathol 147, 1068 (1995) , W W. Hancock, D H. Adams, L R Wyner M. H Sayeh, M J Kamovsky, ibid. 145, 1008 (1994 ). J. D Marmur et al., J. Clin. Invest 91, 2253 (1993), M. W. Majesky, V. Lindner, D. R. Twardzik, S. M. Schwartz, M. A. Reidy, ibid. 88, 904 (1991)
    • (1995) Am. J. Pathol , vol.147 , pp. 1068
    • Reed, M.J.1
  • 31
    • 0027946012 scopus 로고
    • M. J. Reed et al., Am. J. Pathol 147, 1068 (1995) , W W. Hancock, D H. Adams, L R Wyner M. H Sayeh, M J Kamovsky, ibid. 145, 1008 (1994 ). J. D Marmur et al., J. Clin. Invest 91, 2253 (1993), M. W. Majesky, V. Lindner, D. R. Twardzik, S. M. Schwartz, M. A. Reidy, ibid. 88, 904 (1991)
    • (1994) Am. J. Pathol , vol.145 , pp. 1008
    • Hancock, W.W.1    Adams, D.H.2    Wyner, L.R.3    Sayeh, M.H.4    Kamovsky, M.J.5
  • 32
    • 0027319446 scopus 로고
    • M. J. Reed et al., Am. J. Pathol 147, 1068 (1995) , W W. Hancock, D H. Adams, L R Wyner M. H Sayeh, M J Kamovsky, ibid. 145, 1008 (1994 ). J. D Marmur et al., J. Clin. Invest 91, 2253 (1993), M. W. Majesky, V. Lindner, D. R. Twardzik, S. M. Schwartz, M. A. Reidy, ibid. 88, 904 (1991)
    • (1993) J. Clin. Invest , vol.91 , pp. 2253
    • Marmur, J.D.1
  • 33
    • 0026075910 scopus 로고
    • M. J. Reed et al., Am. J. Pathol 147, 1068 (1995) , W W. Hancock, D H. Adams, L R Wyner M. H Sayeh, M J Kamovsky, ibid. 145, 1008 (1994 ). J. D Marmur et al., J. Clin. Invest 91, 2253 (1993), M. W. Majesky, V. Lindner, D. R. Twardzik, S. M. Schwartz, M. A. Reidy, ibid. 88, 904 (1991)
    • (1991) J. Clin. Invest , vol.88 , pp. 904
    • Majesky, M.W.1    Lindner, V.2    Twardzik, D.R.3    Schwartz, S.M.4    Reidy, M.A.5
  • 34
    • 13344266683 scopus 로고    scopus 로고
    • note
    • An electrophoretic mobility-shift assay (EMSA) was done in buffer containing 50 mM NaCl, 1 mM EDTA 10 mM Tris-HCI (pH 7 5), 5% glycerol, 0 5% Nonidet-P40 (Sigma), 1 mM dithiothreitol, and 20 μg of bovine serum albumin (BSA) for 30 min at 22°C.
  • 35
    • 13344289807 scopus 로고    scopus 로고
    • note
    • 35S-undine 5′-triphosphate (UTPJ-labeled riboprobe for 16 hours. After washing, the slides were coated with autoradiographic emulsion and exposed for 3 weeks. The images were photographed and digitized The hybridization signal of the radiolabeled probe appears as white grains All specimens were observed under dark field illumination after nuclear counterstain with hematoxylin. Immunostainmg for factor VIII-related antigen (4) confirmed that injury was limited to endothelium
  • 36
    • 13344267407 scopus 로고    scopus 로고
    • note
    • 2, 5% glycerol, 2 5 mM Hepes (pH 7.9), 1 μg of polydeoxyinosinic-deoxycytidylic acid, and 20 μg of BSA for 30 min at 22°C Polyclonal antipeptide antibodies to Sp1 and Egr-1 (Santa Cruz Biotechnology, Santa Cruz, CA) were incubated with nuclear extracts 15 min before the addition of the probe.
  • 37
    • 13344251088 scopus 로고    scopus 로고
    • note
    • d77mEgr-CAT was constructed with the use of an oligonucleotide bearing the Oligo Bm (12) sequence as the 5′ primer for the polymerase chain reaction. Transfections in BAECs were performed with 10 μg of reporter plasmid and the calcium phosphate protocol (10). The cells were incubated with PMA (100 ng/ml), cotransfected with CMV-Egr-1, or injured with a sterile comb (8), and then incubated for 36 hours at 37°C. CAT activity was assessed by the two-phase fluor diffusion technique (13) and was normalized to the amounts of protein in the cell lysate
  • 38
    • 13344255453 scopus 로고    scopus 로고
    • note
    • 32P-Oligo B (72) for 30 min at 22°C and applied to a running nondenaturing 5% polyacrylamide gel at the times indicated Alternatively, a 1000-fold molar excess of the unlabeled cognate was added after the 30-min incubation and applied to the gel (21).
  • 39
    • 13344255452 scopus 로고    scopus 로고
    • note
    • 32P-Oligo B was incubated with a fixed concentration of Sp1 and decreasing amounts of Egr-1 (Fig 3B, right side) (21).
  • 40
    • 13344289071 scopus 로고    scopus 로고
    • note
    • Protein immunoblots were analyzed with polyclonal antibodies to Egr-1 and Sp1 (1 2500: Santa Cruz Biotechnology) and to PDGF-B (1:200; Genzyme) Immunoreactive proteins were detected by enhanced chemiluminescence (Amersham) with horseradish peroxidase-linked donkey secondary antiserum to rabbit immunoglobulin at 1 10,000 dilution.
  • 44
    • 13344265902 scopus 로고    scopus 로고
    • note
    • We thank F J. Rauscher III for recombinant Egr-1, V. P. Sukhatme for CMV-Egr-1, M. A Frosch for critical review of the manuscript, and M. A. Gimbrone Jr. for enthusiastic support. Supported in part by grants from NIH (T.C.) and the American Heart Association (V.L.). L M.K. is a C. J. Martin Postdoctoral Research Fellow (National Health and Medical Research Council of Australia) and a recipient of a J William Fulbright Postdoctoral Research Award T.C. is an Established Investigator of the American Heart Association.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.