Conferring aldosterone synthesis to human CYP11B1 by replacing key amino acid residues with CYP11B2-specific ones

European Journal of Biochemistry

Volumn 252, Issue 3, 1998, Pages 458-466

  Böttner, Benjamin ^a   Denner, Karsten ^a   Bernhardt, Rita ^a,b  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

Author keywords

Aldosterone synthesis; CYP11B1; CYP11B2; Engineering substrate specificity; Regiospecificity of steroid hydroxylation

Indexed keywords

CHIMERIC PROTEIN; STEROID 11BETA MONOOXYGENASE;

ALDOSTERONE BLOOD LEVEL; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BINDING SITE; ENZYME ACTIVITY; HORMONE SYNTHESIS; HYDROXYLATION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; STEROIDOGENESIS;

ALDOSTERONE; ALDOSTERONE SYNTHASE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; COS CELLS; DNA PRIMERS; HUMANS; HYDROXYLATION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE DELETION; SEQUENCE HOMOLOGY, AMINO ACID; STEROID 11-BETA-HYDROXYLASE; TRANSFECTION;

ANIMALIA;

EID: 0032521610     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2520458.x     Document Type: Article

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