Expression and characterization of recombinant type 2 3α- hydroxysteroid dehydrogenase (HSD) from human prostate: Demonstration of bifunctional 3α/17β-HSD activity and cellular distribution

Molecular Endocrinology

Volumn 11, Issue 13, 1997, Pages 1971-1984

  Lin, Hsueh Kung ^a   Jez, Joseph M ^a   Schlegel, Brian P ^a   Peehl, Donna M ^b   Pachter, Jonathan A ^c   Penning, Trevor M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3ALPHA HYDROXYSTEROID DEHYDROGENASE;

AMINO ACID SEQUENCE; ARTICLE; CELLULAR DISTRIBUTION; COLON; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME LOCALIZATION; ESCHERICHIA COLI; HUMAN; HUMAN TISSUE; KIDNEY; LIVER; LUNG; NONHUMAN; PRIORITY JOURNAL; PROSTATE; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; SMALL INTESTINE; TISSUE DISTRIBUTION;

ESCHERICHIA COLI;

EID: 0030784509     PISSN: 08888809     EISSN: None     Source Type: Journal    
DOI: 10.1210/mend.11.13.0026     Document Type: Article

References (50)

1. Bruchovsky N, Wilson JD 1968 The conversion of testosterone to 5α-androstan-17β- ol-3-one by rat prostate in vivo and in vitro. J Biol Chem 243:2012-2021
2. Wilson JD, Gloyna RE 1970 The intranuclear metabolism of testosterone in the accessory organs of reproduction. Recent Prog Horm Res 26:309-336
3. Fang S, Anderson KM, Liao S 1969 Receptor proteins for androgens: on the role of specific proteins in selective retention of 17β-hydroxy-5α-androstan-3-one by rat ventral prostate in vivo and in vitro. J Biol Chem 244:6584-6596
4. Mainwaring WI 1969 A soluble androgen receptor in the cytoplasm of rat prostate. J Endocrinol 45:531-541
5. Siiteri PK, Wilson JD 1970 Dihydrotestosterone in prostatic hypertrophy, I: the formation and content of dihydrotestosterone in the hypertrophic prostate of man. J Clin Invest 49:1737-1745
6. Gloyna R, Siiteri PK, Wilson JD 1970 Dihydrotestosterone in prostatic hypertrophy, II: the formation and content of dihydrotestosterone in the hypertrophic canine prostate and the effect of dihydrotestosterone on prostate growth in the dog. J Clin Invest 49:1746-1753
7. Lloyd JW, Thomas JA, Mawhinney MG 1975 Androgens and estrogens in the plasma and prostatic tissue of normal dogs and dogs with benign prostatic hypertrophy. Invest Urol 13:202-222
8. Hammond GL 1978 Endogenous steroid levels in the human prostate from birth to old age: a comparison of normal and diseased tissues. J Endocrinol 78:7-19
9. Meikle AW, Collier ES, Stringham JD, Fang S, Taylor GN 1981 Elevated intranuclear dihydrotestosterone in prostatic hyperplasia of aging dogs. J Steroid Biochem 14:331-335
10. Geller J, Albert J, Loza D, Geller S, Stoeltzing W, de la Vega D 1978 Dihydrotestosterone concentrations in human prostatic cancer tissues. J Clin Endocrinol Metab 46:440-444
11. Krieg M, Bartsch W, Janssen W, Voigt KD 1979 A comparitive study of binding, metabolism, and endogenous levels of androgens in normal, hyperplastic, and carcinomatous human prostate. J Steroid Biochem 11:615-624
12. Liao S, Liang T, Fang S, Castaneda E, Shao TC 1973 Steroid structure and androgenic activity: specificities involved in the receptor binding and nuclear retention of various androgens. J Biol Chem 248:6154-6162
13. Issacs JT 1983 Changes in dihydrotestosterone metabolism and the development of benign prostatic hyperplasia in the aging beagle. J Steroid Biochem 18:749-757
14. McKercher G, Chevalier S, Roberts KD, Bleau G, Chapdelaine A 1984 5α-Reductase and 3α-hydroxysteroid dehydrogenase activities in isolated canine prostatic epithelial cells. J Steroid Biochem 21:549-554
15. Span PN, Sweep CGJ, Benraad TJ, Smals AGH 1996 3α-Hydroxysteroid oxidoreductase activities in dihydrotestosterone degradation and back-formation in rat prostate and epididymis. J Steroid Biochem Mol Biol 58:319-324
16. + oxidoreductase of rat ventral prostate. Biochemistry 14:810-817
17. Inano H, Hayahi S, Tamaoki B 1977 Prostate 3α-hydroxysteroid dehydrogenase: its partial purification and properties. J Steroid Biochem 8:41-46
18. Jacobi GH, Moore RJ, Wilson JD 1978 Studies on the mechanism of 3α-androstanediol-induced growth of the dog prostate. Endocrinology 102:1748-1755
19. Krieg M, Weisser H, Tunn S 1995 Potential activities of androgen metabolizing enzymes in human prostate. J Steroid Biochem Mol Biol 53:395-400
20. Jacobi GH, Moore RJ, Wilson JD 1977 Characterization of the 3α-hydroxysteroid dehydrogenase of dog prostate. J Steroid Biochem 8:719-723
21. Hudson RW 1982 Studies of cytosol 3α-hydroxysteroid dehydrogenase of human prostatic tissue: comparison of enzyme activities in hyperplastic malignant and normal tissues. J Steroid Biochem 16:373-377
22. Hudson RW 1984 Comparison of 3α-hydroxysteroid dehydrogenase activities in the microsomal fractions of hyperplastic malignant and normal human prostatic tissues. J Steroid Biochem 20:829-833
23. Amet Y, Simon B, Quemener E, Mangin P, Floch HH, Abalain JH 1992 Partial purification of 3α- and 3β-hydroxysteroid dehydrogenases from human hyperplastic prostate: comparison between the two enzymes. J Steroid Biochem Mol Biol 41:689-692
24. Penning TM, Pawlowski JE, Schlegel BP, Jez JM, Lin H-K, Smith-Hoog S, Bennett MJ, Lewis M 1996 Mammalian 3α-hydroxysteroid dehydrogenases. Steroids 61:508-523
25. Jez JM, Flynn TG, Penning TM 1996 A nomenclature system for the aldo-keto reductase superfamily. In: Weiner H, Lindahl R, Crabb DW, Flynn TG (eds) Enzymology and Molecular Biology of Carbonyl Metabolism. Plenum Press, New York, vol 6:579-589
26. Pawlowski JE, Huizinga M, Penning TM 1991 Cloning and sequencing of the cDNA for rat liver 3α-hydroxysteroid/dihydrodiol dehydrogenase. J Biol Chem 266:8820-8825
27. Khanna M, Qin KN, Wang RW, Cheng KC 1995 Substrate specificity, gene structure, and tissue-specific distribution of multiple human 3α-hydroxysteroid dehydrogenases. J Biol Chem 270:20162-20168
28. Dufort I, Soucy P, Labrie F, Luu-The V 1996 Molecular cloning of human type 3 3α-hydroxysteroid dehydrogenase that differs from 20α-hydroxysteroid dehydrogenase by seven amino acids. Biochem Biophys Res Commun 228:474-479
29. Pawlowski JE, Penning TM 1994 Overexpression and mutagenesis of the cDNA for rat liver 3α-hydroxysteroid/dihydrodiol dehydrogenase. J Biol Chem 269: 13502-13510
30. Hoog SS, Pawlowski JE, Alzari PM, Penning TM, Lewis M 1994 Three-dimensional structure of rat liver 3α-hydroxysteroid/dihydrodiol dehydrogenase: a member of the aldo-keto reductase superfamily. Proc Natl Acad Sci USA 91:2517-2521
31. +. Biochemistry 35:10702-10711
32. + bound to 3α-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase. Structure 5:799-812
33. Jez JM, Schlegel BP, Penning TM 1996 Characterization of the substrate binding site in rat liver 3α-hydroxysteroid/dihydrodiol dehydrogenase: the roles of tryptophans in ligand recognition and protein fluorescence. J Biol Chem 271:30190-30198
34. Bruchovsky N, Sadar MA, Akafura K, Goldenberg SL, Matsuoka K, Rennie PS 1996 Characterization of 5α-reductase gene expression in stroma and epithelium of human prostate. J Steroid Biochem Mol Biol 59:397-404
35. Hara A, Matsuura K, Tamada Y, Sato K, Miyabe Y, Deyashiki Y, Ishida N 1996 Relationship of human liver dihydrodiol dehydrogenases to hepatic bile acid binding protein and an oxidoreductase of human colon cells. Biochem J 313:373-376
36. Stolz A, Hammond L, Lou H, Takikawa H, Ronk M, Shively JE 1993 cDNA cloning and expression of human hepatic bile-acid binding protein. J Biol Chem 268: 10448-10457
37. Deyashiki Y, Ogasawara A, Nakayama T, Nakanishi M, Miyabe Y, Sato K, Hara A 1994 Molecular cloning of two human liver 3α-hydroxysteroid/dihydrodiol dehydrogenase isoenzymes that are identical with chlordecone reductase and bile-acid binder. Biochem J 299:545-552
38. Winters CJ, Molowa DT, Guzelian PS 1990 Isolation and characterization of cloned cDNAs encoding human liver chlordecone reductase. Biochemistry 29:1080-1087
39. Dufort I, Soucy P, Zhang Y, Luu-The V, Cloning and characterization of human type 2 3α-hydroxysteroid dehydrogenase from human prostatic cDNA library. Program of the Fifth International Congress on Hormones, Cancer, Quebec, Canada, 1995, p 133 (Abstract)
40. Hara A, Taniguchi H, Nakayama T, Sawada H 1990 Purification and properties of multiple forms of dihydrodiol dehydrogenase from human liver. J Biochem 108:250-254
41. Labrie F, Luu-The V, Lin SX, Labrie C, Simard J, Breton R, Belanger A 1997 The key role of 17β-hydroxysteroid dehydrogenases in sex steroid biology. Steroids 62:148-158
42. Cowan RA, Cowan SK, Grant JK, Elder HY 1977 Biochemical investigations of separated epithelium and stroma from benign hyperplastic prostatic tissue. J Endocrinol 74:111-120
43. Lasnitzki I, Mizuno T 1979 Role of the mesenchyme in the induction of the rat prostate gland by androgens in organ culture. J Endocrinol 82:171-178
44. Orlowski J, Bird CE, Clark AF 1983 Androgen 5α-reductase and 3α-hydroxysteroid dehydrogenase activities in ventral prostate epithelial and stromal cells from immature and mature rats. J Endocrinol 99:131-139
45. Levine AC, Wang JP, Ren M, Eliashvili E, Russell DW, Kirschenbaum A 1996 Immunohistochemical localization of steroid 5α-reductase-2 in the human male reproductive-tract and adult prostate. J Clin Endocrinol Metab 81:384-389
46. Ciaccio PJ, Tew KD 1994 cDNA and deduced amino acid sequence of a human colon dihydrodiol dehydrogenase. Biochim Biophys Acta 1186:129-132
47. Leatherbarrow RJ 1987 ENZFITTER: A Non-linear Regression Data Analysis Program for the IBM PC (and true compatibles). BioSoft, Cambridge, UK
48. Wilkinson GN 1961 Statistical estimations in enzyme kinetics. Biochem J 80:324-332
49. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ 1990 Basic local alignment search tool. J Mol Biol 215: 403-410
50. Kabalin JN, Peehl DM, Stamey TA 1989 Clonal growth of human prostatic epithelial cells is stimulated by fibroblasts. Prostate 14:251-263