Calcium binding to the first EGF-like module of human factor IX in a recombinant fragment containing residues 1-85: Mutations V46E and Q50E each manifest a negligible increase in calcium affinity

FEBS Letters

Volumn 421, Issue 2, 1998, Pages 100-104

  Persson, Kristina E M ^a   Astermark, Jan ^a   Björk, Ingemar ^b   Stenflo, Johan ^a  

^a LUND UNIVERSITY   (Sweden)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

Calcium binding; Dissociation constant; EGF like module; Human factor IX; Site directed mutagenesis

Indexed keywords

4 CARBOXYGLUTAMIC ACID; BLOOD CLOTTING FACTOR 9; CALCIUM ION; EPIDERMAL GROWTH FACTOR; GLUTAMIC ACID; RECOMBINANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CALCIUM BINDING; DISSOCIATION CONSTANT; MUTATION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; CALCIUM; CELL LINE; EPIDERMAL GROWTH FACTOR; FACTOR IX; GLUTAMIC ACID; GLUTAMINE; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; RECOMBINANT FUSION PROTEINS; SPODOPTERA; VALINE;

EID: 0032498121     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(97)01546-9     Document Type: Article

References (32)

1. Furie B., Furie B.C. Cell. 53:1988;505-518.
2. Davie E.W., Fujikawa K., Kisiel W. Biochemistry. 30:1991;10363-10370.
3. Brandsetter H., Bauer M., Huber R., Lollar P., Bode W. Proc. Natl. Acad. Sci. USA. 92:1995;9796-9800.
4. Banner D.W., D'Arcy A., Chène C., Winkler F.K., Guha A., Koningsberg W.H., Nemerson Y., Kirchhofer D. Nature. 380:1996;41-46.
5. Sunnerhagen M., Olah G.A., Stenflo J., Forsen S., Drakenberg T., Trewhella J. Biochemistry. 35:1996;11547-11559.
6. Sunnerhagen M., Forsén S., Hoffrén A.-M., Drakenberg T., Teleman O., Stenflo J. Nature Struct. Biol. 2:1995;504-509.
7. Giannelli F., et al. Nucleic Acids Res. 24:1996;103-118.
8. Handford P.A., Mayhew M., Baron M., Winship P.R., Campbell I.D., Brownlee G.G. Nature. 351:1991;164-167.
9. Rees D.J.G., Jones I.M., Handford P.A., Walter S.J., Esnouf M.P., Smith K.J., Brownlee G.G. EMBO J. 7:1988;2053-2061.
10. Persson E., Selander M., Linse S., Drakenberg T., Ohlin A.K., Stenflo J. J. Biol. Chem. 264:1989;16897-16904.
11. Valcarce C., Selander-Sunnerhagen M., Tämlitz A.-M., Drakenberg T., Björk I., Stenflo J. J. Biol. Chem. 268:1993;26673-26678.
12. Baron M., Norman D.G., Harvey T.S., Handford P.A., Mayhew M., Tse A.G.D., Brownlee G.G., Campbell I.D. Protein Sci. 1:1992;81-90.
13. Rao Z., Handford P., Mayhew M., Knott V., Brownlee G.G., Stuart D. Cell. 82:1995;131-141.
14. Selander Sunnerhagen M., Ullner M., Persson E., Teleman O., Stenflo J., Drakenberg T. J. Biol. Chem. 267:1992;19642-19649.
15. Astermark J., Sottile J., Mosher D.F., Stenflo J. J. Biol. Chem. 269:1994;3690-3697.
16. Stenberg, Y., Dahlbäck, B. and Stenflo, J. (1997) Eur. J. Biochem. (in press).
17. Laemmli U.K. Nature. 227:1970;680-684.
18. Rand M.D., Lindblom A., Carlson J., Villoutreix B.O., Stenflo J. Protein Sci. 6:1997;1-13.
19. Nordenman B., Björk I. Biochemistry. 17:1978;3339-3344.
20. Sottile J., Mosher D.F. Biochemistry. 32:1993;1641-1647.
21. Hase S., Kawabata S.-I., Nishimura H., Takeya H., Sueyoshi T., Miyata T., Iwanaga S., Takao T., Shimonoshi Y., Ikenaka T. J. Biochem. 104:1988;867-868.
22. Nishimura H., Takao T., Hase S., Shimonishi Y., Iwanaga S. J. Biol. Chem. 267:1992;17520-17525.
23. Roth D.A., Rehemtulla A., Kaufman R.J., Walsh C.T., Furie B., Furie B.C. Proc. Natl. Acad. Sci. USA. 90:1993;8372-8376.
24. Astermark J., Björk I., Öhlin A.-K., Stenflo J. J. Biol. Chem. 266:1991;2430-2437.
25. Fernlund P., Stenflo J. J. Biol. Chem. 258:1983;12509-12512.
26. Snell E., Boyer P., Meister A., Richardsson C. Annu. Rev. Biochem. 46:1997;157-168.
27. Handford P.A., Baron M., Mayhew M., Willis A., Beesley T., Brownlee G.G., Campbell I.D. EMBO J. 9:1990;475-480.
28. Stenflo J. Blood. 78:1991;1637-1651.
29. Kelly C.R., Dickinson C.D., Ruf W. J. Biol. Chem. 272:1997;17467-17472.
30. Stenberg Y., Linse S., Drakenberg T., Stenflo J. J. Biol. Chem. 272:1997;23255-23260.
31. Knott V., Downing A.K., Cardy C.M., Handford P. J. Mol. Biol. 255:1996;22-27.
32. Linse S., Brodin P., Johansson C., Thulin E., Grundström T., Forsén S. Nature. 335:1988;651-652.