Expression and purification of the seven nonstructural proteins of the flavivirus Kunjin in the E. coli and the baculovirus expression systems

Journal of Virological Methods

Volumn 61, Issue 1-2, 1996, Pages 47-58

  Khromykh, A A ^a   Harvey, T J ^a   Abedinia, M ^b   Westaway, E G ^a  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Baculovirus; Expression; Fusion proteins; Kunjin virus; Non structural proteins

Indexed keywords

HYBRID PROTEIN; VIRUS PROTEIN; VIRUS VECTOR;

ARTICLE; BACULOVIRUS; CONTROLLED STUDY; ESCHERICHIA COLI; FLAVIVIRUS; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ESCHERICHIA COLI; FLAVIVIRUS; KUNJIN VIRUS; SPODOPTERA FRUGIPERDA; UNIDENTIFIED BACULOVIRUS;

EID: 0030249999     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(96)02068-X     Document Type: Article

References (42)

1. Beekman, J.M., Cooney, A.J., Elliston, J.F., Tsai, S.Y. and Tsai, M.J. (1994) A rapid one-step method to purify baculovirus-expressed human estrogen receptor to be used in the analysis of the oxytocin promoter. Gene 146, 285-289.
2. Biedrzycka, A., Cauchi, M.R., Bartholomeusz, A., Gorman, J.J. and Wright, P.J. (1987) Characterization of protease cleavage sites involved in the formation of the envelope glycoprotein and three non-structural proteins of dengue virus type 2, New Guinea strain. J. Gen. Virol. 68, 1317-1326
3. Cahour, A., Falgout, B. and Lai, C.J. (1992) Cleavage of the dengue virus polyprotein at the NS3/NS4A and NS4B/NS5 junctions is mediated by viral protease NS2B-NS3, whereas NS4A/NS4B may be processed by a cellular protease. J. Virol. 66, 1535-1542.
4. Chambers, T.J., Grakoui, A. and Rice, C.M. (1991) Processing of the yellow fever virus nonstructural polyprotein: a catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites. J. Virol. 65, 6042-6050.
5. Chambers, T.J., McCourt, D.W. and Rice, C.M. (1989) Yellow fever virus proteins NS2A, NS2B, and NS4B: identification and partial N-terminal amino acid sequence analysis. Virology 169, 100-109.
6. Chambers, T.J., Hahn, C.S., Galler, R. and Rice, C.M. (1990a) Flavivirus genome organization, expression, and replication. Annu. Rev. Microbiol. 44, 649-688.
7. Chambers, T.J., McCourt, D.W. and Rice, C.M. (1990b) Production of yellow fever virus proteins in infected cells: identification of discrete polyprotein species and analysis of cleavage kinetics using region-specific polyclonal antisera. Virology 177, 159-174.
8. Coia, G., Parker, M.D., Speight, G., Byrne, M.E. and Westaway, E.G. (1988) Nucleotide and complete amino acid sequences of Kunjin virus: definitive gene order and characteristics of the virus-specified proteins. J. Gen. Virol. 69, 1-21.
9. Davies, A.H., Jowett, J.B.M. and Jones, I.M. (1993) Recombinant baculovirus vectors expressing glutathione-S-transferase fusion proteins. Bio/Technology 11, 933-936.
10. Falgout, B., Pethel, M., Zhang, Y.M. and Lai, C.J. (1991) Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J. Virol. 65, 2467-2475.
11. Gorbalenya, A.E., Donchenko, A.P., Koonin, E.V. and Blinov, V.M. (1989) N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases. Nucleic Acids Res. 17, 3889-3897.
12. Gorbalenya, A.E., Koonin, E.V., Donchenko, A.P. and Blinov, V.M. (1989b) Two related superfamilies of putative helicases involved in replication, recombination, repair, and expression of DNA and RNA genomes. Nucleic Acids Res. 17, 4713-4730.
13. Hanke, T., Bolting, C., Green, E.A., Szawlowski, P.W., Rud, E. and Randall, R.E. (1994) Expression and purification of nonglycosylated SIV proteins, and their use in induction and detection of SIV-specific immune responses. AIDS Res. Hum. Retroviruses 10, 665-674.
14. Harris, M.P. and Coates, K. (1993) Identification of cellular proteins that bind to the human immunodeficiency virus type 1 nef gene product in vitro: a role for myristylation. J. Gen. Virol. 74, 1581-1589.
15. Hay, J.M. and Hull, R. (1992) An improved procedure for the purification of protein fused with glutathione-S-transferase. BioTechniques 13, 856-857.
16. Jan, L-R., Yang., C-S., Trent, D.W., Falgout, B. and Lai, C.-J. (1995) Processing of Japanese encephalitis virus non-structural proteins: NS2B-NS3 complex and heterologous proteases. J. Gen. Virol. 76, 573-580.
17. Khromykh, A.A. and Westaway, E.G. (1994) Completion of Kunjin virus RNA sequence and recovery of an infectious RNA transcribed from stably cloned full-length cDNA. J. Virol. 68, 4580-4588.
18. Khromykh, A.A., Meka, H. and Westaway, E.G. (1995) Preparation of recombinant baculovirus by transfection of a ligated cDNA fragment without prior plasmid amplification in E. coli. BioTechniques 12, 356-360.
19. Kitts, P.A. and Possee, R.D. (1993) A method for producing recombinant baculovirus expression vectors at high frequency. BioTechniques 14, 810-817.
20. Koonin, E.V. (1993) Computer-assisted identification of a putative methyltransferase domain in NS5 protein of flaviviruses and lambda 2 protein of reovirus. J. Gen. Virol. 74, 733-740.
21. Kwang, J., Littledike, E.T., Donis, R.O. and Dubovi, E.J. (1992) Recombinant polypeptide from the gp48 region of the bovine viral diarrhea virus (BVDV) detects serum antibodies in vaccinated and infected cattle. Vet. Microbiol. 32, 281-292.
22. Matsuura, Y., Possee, R., Overton, H.A. and Bishop, D.H.L. (1987) Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. J. Gen. Virol. 68, 1233-1250.
23. Parrish, C.R., Coia, G., Hill, A., Mullbacher, A., Westaway, E.G. and Blanden, R.V. (1991) Preliminary analysis of murine cytotoxic T cell responses to the proteins of the flavivirus Kunjin using vaccinia virus expression. J. Gen. Virol. 72, 1645-1653.
24. Preugschat, F., Yao, C.W. and Strauss, J.H. (1990) In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3. J. Virol. 64, 4364-4374.
25. Pugachev, K.V., Pletnev, A.G. and Matveev, L.E. (1992) Expression of the gene for tick-borne viral encephalitis virus NS3 protein in Escherichia coli cells. Mol. Biol. Mosk. 26, 158-167 (in Russian).
26. Rice, C.M., Lenches, E.M., Eddy, S.R., Shin, S.J., Sheets, R.L. and Strauss, J.H. (1985) Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229, 726-733.
27. Sankar, S. and Porter, A.G. (1991) Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependant RNA polymerase. J. Virol. 65, 2993-3000.
28. Smith, D.B. and Johnson, K.S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
29. Speight, G., Coia, G., Parker, M.D. and Westaway, E.G. (1988) Gene mapping and positive identification of the non-structural proteins NS2A, NS2B, NS3, NS4B and NS5 of the flavivirus Kunjin and their cleavage sites. J. Gen. Virol. 69, 23-34.
30. Speight, G. and Westaway, E.G. (1989a) Carboxy-terminal analysis of nine proteins specified by the flavivirus Kunjin: evidence that only the intracellular core protein is truncated. J. Gen. Virol. 70, 2209-2214.
31. Speight, G. and Westaway, E.G. (1989b) Positive identification of NS4A, the last of the hypothetical nonstructural proteins of flaviviruses. Virology 170, 299-301.
32. Takegami, T., Sakamuro, D. and Furukawa, T. (1994) Japanese encephalitis virus nonstructural protein NS3 has RNA binding and ATPase activities. Virus Genes 9, 105-112.
33. Wan, M. and Loh, B.N. (1995) Expression and purification of active form of HIV-1 protease from E. coli. (1995) Biochem. Mol. Biol. Int. 35, 899-912.
34. Wang, Y-H., Davies, A.H. and Jones, I.M. (1995) Expression and purification of glutathione S-transferase-tagged HIV-1 gp120: no evidence of an interaction with CD26. Virology 208, 142-146.
35. Warrener, P., Tamura, J.K. and Collett, M.S. (1993) RNA-stimulated NTPase activity associated with yellow fever virus NS3 protein expressed in bacteria. J. Virol. 67, 989-996.
36. Weiss, S., Famulok, M., Edenhofer, F., Wang, Y.H., Jones, I.M., Groschup, M. and Winnacker, E.L. (1995) Overexpression of active Syrian golden hamster prion protein PrPc as a glutathione S-transferase fusion in heterologous systems. J. Virol. 69, 4776-4783.
37. Wengler, G. and Wengler, G. (1991) The carboxy-terminal part of the NS 3 protein of the West Nile flavivirus can be isolated as a soluble protein after proteolytic cleavage and represents an RNA-stimulated NTPase. Virology 184, 707-715.
38. Wengler, G. and Wengler, G. (1993) The NS3 nonstructural protein of flaviviruses contains an RNA triphosphatase activity. Virology 197, 265-73.
39. Wengler, G., Czaya, G., Farber, P.M. and Hegemann, J.H. (1991) In vitro synthesis of West Nile virus proteins indicates that the amino-terminal segment of the NS3 protein contains the active centre of the protease which cleaves the viral polyprotein after multiple basic amino acids. J. Gen. Virol. 72, 851-858.
40. Westaway, E.G., Brinton, M.A., Gaidamovich, S.Y., Horzinek, M.C., Igarashi, A., Kaariainen, L., Lvov, D.K., Porterfield, J.S., Russell, P.K. and Trent, D.W. (1985) Togaviridae. Intervirology 24, 125-139.
41. Yu, M., Gould, A.R., Morrissy, C.J. and Westbury, H.A. (1994) High level expression of the envelope glycoprotein (gp53) of bovine viral diarrhoea virus (Singer) and its potential use as a diagnostic reagent. Virus. Res. 34, 178-186.
42. Zhang, L. and Padmanabhan, R. (1993) Role of protein conformation in the processing of dengue virus type 2 nonstructural polyprotein precursor. Gene 129, 197-205.