Intracellular localisation of dengue-2 RNA in mosquito cell culture using electron microscopic in situ hybridisation

Archives of Virology

Volumn 142, Issue 12, 1997, Pages 2347-2357

  Grief, C ^a,b   Galler, R ^a   Cortes L M C ^a   Barth, O M ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b FEDERAL UNIVERSITY OF MATO GROSSO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

AEDES; ANIMAL; ARTICLE; CELL LINE; DENGUE VIRUS; ELECTRON MICROSCOPY; GENETICS; HUMAN; IN SITU HYBRIDIZATION; METHODOLOGY; PHYSIOLOGY; TIME; ULTRASTRUCTURE; VIROLOGY; VIRUS REPLICATION;

AEDES; ANIMALS; CELL LINE; DENGUE VIRUS; HUMANS; IN SITU HYBRIDIZATION; MICROSCOPY, ELECTRON; RNA, VIRAL; TIME FACTORS; VIRUS REPLICATION;

EID: 0031445610     PISSN: 03048608     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007050050247     Document Type: Article

References (20)

1. An SF, Franklin D, Flemming KA (1992) Generation of digoxigenin-labelled double-stranded and single-stranded probes using the polymerase chain reaction. Mol Cell Probes 6: 193-200
2. Barth OM (1992) Replication of dengue viruses in mosquito cell cultures-a model from ultrastructural observations. Mem Inst Oswaldo Cruz 87: 565-574
3. Bienz K, Egger D, Pasamontes L (1987) Association of poliovirus proteins of the P2 genomic region with the viral replication complex and virus induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography. Virology 160: 220-226
4. Bienz K, Egger D, Pfister T, Troxler M (1992) Structural and functional characterization of the poliovirus replication complex. J Virol 66: 2 740-2 747
5. Caliguiri LA, Tamm I (1970) Characterization of poliovirus specific structures associated with cytoplasmic membranes. Virology 42: 112-122
6. Chu PWG, Westaway EG (1992) Molecular and structural analysis of heavy membrane fractions associated with the replication of Kunjin virus RNA. Arch Virol 125: 177-191
7. Etchison D, Ehrenfeld E (1981) Comparison of replication complexes synthesizing poliovirus RNA. Virology 111: 33-46
8. Fields BN, Knipe DM (1990) Virology. Raven Press, New York
9. Grief C, Farrar GH, Kent KA, Berger EG (1991) The assembly of HIV within the Golgi apparatus and Golgi derived vesicles of JM cell syncytia. AIDS 5: 1 433-1 439
10. Hahn YS, Galler R, Hunkapillar T, Dalrymple J, Strauss JH, Srauss EG (1988) Nucleotide sequence of dengue 2 RNA and comparison of encoded proteins with those of other flaviviruses. Virology 162: 167-180
11. Hase T, Summers PL, Eckels KH (1989) Morphogenesis of flavivirus. Subcell Biochem 15: 275-305
12. Henchal E A, Putnic JR (1990) The dengue viruses. Clin Microbiol Rev 3: 376-396
13. Mackenzie JM, Jones MK, Young PR (1996) Immunolocalization of the non-structural glycoprotein NS1 suggests a role in viral RNA replication. Virology 220: 232-240
14. Nogueira RMR, Miagostovich MP, Lampe E, Schatzmayr HG (1990) Isolation of dengue virus type 2 in Rio de Janeiro. Mem Inst Oswaldo Cruz 85: 253
15. Ng ML, Corner LC (1989) Detection of some dengue-2 virus antigens in infected cells using immuno-microscopy. Arch Virol 104: 197-208
16. Ng ML, Yeong FM, Tan SH (1994) Cryosubstitution technique reveals new morphology of flavivirus-induced structures. J Virol Methods 49: 305-314
17. Schatzmayer HG, Nogueira RMR, Travassos Da Rosa APA (1986) An outbreak of dengue virus in Rio de Janeiro. Mem Inst Oswaldo Cruz 81: 245-246
18. Troxler M, Pasamontes C, Egger D, Bienz K (1990) In situ hybridization for light and electron microscopy a comparison of methods for the localization of viral RNA using biotinylated DNA and RNA probes. J Virol Methods 30: 1-14
19. Westaway EG (1987) Flavivirus replication strategy. Adv Virus Res 33: 45-90
20. Williams MA (1977) Quantitative methods in biology. In: Glauert AM (ed) Practical methods in electron microscopy, Vol 6 Part 11. North Holland/Elsevier, New York, pp 203-216