Mixer, a homeobox gene required for endoderm development

Science

Volumn 281, Issue 5373, 1998, Pages 91-96

  Henry, Gilbert L ^a   Melton, Douglas A ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; ENDODERMIN; FATTY ACID BINDING PROTEIN; HOMEODOMAIN PROTEIN; MARKER; MESSENGER RNA; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLASTULA; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; ENDODERM; GASTRULA; GENE ISOLATION; HOMEOBOX; MESODERM; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; XENOPUS LAEVIS;

ANIMALS; BLASTOCYST; CELL LINEAGE; CLONING, MOLECULAR; DNA-BINDING PROTEINS; EMBRYONIC INDUCTION; ENDODERM; GASTRULA; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENES, HOMEOBOX; HIGH MOBILITY GROUP PROTEINS; HOMEODOMAIN PROTEINS; IN SITU HYBRIDIZATION; MESODERM; MOLECULAR SEQUENCE DATA; PROTEINS; RNA, MESSENGER; TRANSCRIPTION FACTORS; XENOPUS LAEVIS; XENOPUS PROTEINS;

ANIMALIA; VERTEBRATA; XENOPUS LAEVIS;

EID: 0032479582     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5373.91     Document Type: Article

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48. 2, 2.5 mM Hepes (pH 7.4)] at 16° to 23°C.
49. RT-PCR was performed as described in (4). Primer sets are either described below or referenced (sequences are 5′ to 3′). All explants, including those used in library construction, were harvested by the Trisolv method. NCAM, GLOBIN, XTWIST, MUSCLE ACTIN, IFABP (4); Xlhbox-8 (9); XBRA [P. A. Wilson and D. A. Melton, Curr. Biol. 4, 676 (1994)]; EDD. ODC (25); LFABP, up-ACCGAGATTGAACAGAATGG; down-CCTCCATGTTTACCACGGAC; MIX. 1, up-CCCAGGCATCATCCAATGTC; down-TGACACGGCTCTTGGTTGGC; MIX-ER. up-CACCAGCCCAGCACTTAACC; down-CAATGTCACATCAACTGAAG; Sox17α, Sox17β (8).
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51. Supported in part by grants from the National Institutes of Health and the Howard Hughes Medical Institute (HHMI). C.L.H. was supported by a predoctoral grant from the National Science Foundation and DAM. is an investigator of the HHMI. We acknowledge the help of R. Harland for advice on expression libraries, K. Symes for histological advice, R. Nicholls and S. Newfield for sequence alignment advice, P. Rahaim and J. Rush of the HHMI Biopolymers facility for both DNA synthesis and sequencing, E. Wu for computing advice, P. Mead for communicating data before publication, H. Woodland and D. Clements for providing Sox17 clones, and all members of the Melton lab for helpful discussions - in particular L. Chen, Z. Balsara, S. Kim, M. Hebrok, O. Kelley, E. Wu, K. O'Donnel, C-J. Lai, J. Wells, P. Klein, D. Kessler, and C. Dohrman.