Recent advances in the molecular biology of nucleoside transporters of mammalian cells

Biochemistry and Cell Biology

Volumn 76, Issue 5, 1998, Pages 761-770

  Cass, Carol E ^a,c   Young, James D ^a   Baldwin, Stephen A ^b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b UNIVERSITY OF LEEDS   (United Kingdom)

^c CROSS CANCER INSTITUTE   (Canada)

Author keywords

CNT; Concentrative; ENT; Equilibrative; Nucleoside transporter

Indexed keywords

CARRIER PROTEIN; COMPLEMENTARY DNA; CONCENTRATIVE NUCLEOSIDE TRANSPORTER; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER; ISOPROTEIN; NITROBENZYLTHIOINOSINE; UNCLASSIFIED DRUG;

CONFERENCE PAPER; HUMAN; MAMMAL CELL; MOLECULAR CLONING; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOSIDE TRANSPORT; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN TERTIARY STRUCTURE; SPECIES DIFFERENCE; TRANSPORT KINETICS;

MAMMALIA; XENOPUS LAEVIS;

EID: 0032467665     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/o98-095     Document Type: Conference Paper

References (68)

1. Agbanyo, F.R., Cass, C.E., and Paterson, A.R. 1988. External location of sites on pig erythrocyte membranes that bind nitrobenzylthioinosine. Mol. Pharmacol. 33: 332-337.
2. Agbanyo, F.R., Vijayalakshmi, D., Craik, J.D., Gati, W.P., McAdam, D.P., Asakura, J., Robins, M.J., Paterson, A.R., and Cass, C.E. 1990. 5′-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine (SAENTA), a novel ligand with high affinity for polypeptides associated with nucleoside transport. Partial purification of the nitrobenzylthioinosine-binding protein of pig erythrocytes by affinity chromatography. Biochem. J. 270: 605-614.
3. Anderson, C.M., Xiong, W., Young, J.D., Cass, C.E., and Parkinson, F.E. 1996. Demonstration of the existence of mRNAs encoding N1/cif and N2/cit sodium/nucleoside cotransporters in rat brain. Mol. Brain Res. 42: 358-361.
4. Belt, J.A. 1983a. Heterogeneity of nucleoside transport in mammalian cells. Two types of transport activity in L1210 and other cultured neoplastic cells. Mol. Pharmacol. 24: 479-484.
5. Belt, J.A. 1983b. Nitrobenzylthioinosine-insensitive uridine transport in human lymphoblastoid and murine leukemia cells. Biochem. Biophys. Res. Commun. 110: 417-423.
6. Belt, J.A., Harper, E.H., Byl, J.A., and Noel, L.D. 1992. Sodium-dependent nucleoside transport in human myeloid leukemic cell lines and freshly isolated myeloblasts. Proc. Am. Assoc. Cancer Res. 33: 20.
7. Belt, J.A., Marina, N.M., Phelps, D.A., and Crawford, C.R. 1993. Nucleoside transport in normal and neoplastic cells. Adv. Enzyme. Regul. 33: 235-252.
8. Boleti, H., Coe, I.R., Baldwin, S.A., Young, J.D., and Cass, C.E. 1997. Molecular identification of the equilibrative NBMPR-sensitive (es) nucleoside transporter and demonstration of an equilibrative NBMPR-insensitive (ei) transport activity in human erythroleukemia (K562) cells. Neuropharmacology, 36: 1167-1179.
9. Boumah, C.E., Hogue, D.L., and Cass, C.E. 1992. Expression of high levels of nitrobenzylthioinosine-sensitive nucleoside transport in cultured human choriocarcinoma (BeWo) cells. Biochem. J. 288: 987-996.
10. Buolamwini, J.K. 1997. Nucleoside transport inhibitors: structure-activity relationships and potential therapeutic applications. Curr. Med. Chem. 4: 35-66.
11. Buolamwini, J.K., Craik, J.D., Wiley, J.S., Robins, M.J., Gati, W.P., Cass, C.E., and Paterson, A.R.P. 1994. Conjugates of fluorescein and SAENTA (5′-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine): flow cytometry probes for the es nucleoside transporter elements of the plasma membrane. Nucleosides Nucleotides, 13: 737-751.
12. Casari, G., Andrade, M.A., Bork, P., Boyle, J., Daruvar, A., Ouzounis, C., Schneider, R., Tamames, J., Valencia, A., and Sander, C. 1995. Challenging times for bioinformatics. Nature (London), 376: 647-648.
13. Cass, C.E. 1995. Nucleoside transport. In Drug transport in antimicrobial therapy and anticancer therapy. Edited by N.H. Georgopapadakou. Marcel Dekker, New York. pp. 403-451.
14. Cass, C.E., Belt, J.A., and Paterson, A.R. 1987. Adenosine transport in cultured cells and erythrocytes. Prog. Clin. Biol. Res. 230: 13-40.
15. Cass, C.E., Young, J.D., Baldwin, S.A., Cabrita, M.A., Graham, K.A., Griffiths, M., Jennings, L.L., Mackey, J.R., Ng, A.M.L., Ritzel, M.W.L., Vickers, M.F., and Yao, S.Y.M. 1998. Nucleoside transporters of mammalian cells. In Membrane transporters as drug targets. Edited by G. Amidon and W. Sadee. Plenum Publishing Corporation, New York. In press.
16. +-nucleoside cotransporter. J. Biol. Chem. 270: 13 596-13 599.
17. Coe, I.R., Griffiths, M., Young, J.D., Baldwin, S.A., and Cass, C.E. 1997. Assignment of the human equilibrative nucleoside transporter (hENT1) to 6p21.1-p21.2. Genomics, 45: 459-460.
18. Conant, A.R., and Jarvis, S.M. 1994. Nucleoside influx and efflux in guinea-pig ventricular myocytes. Inhibition by analogues of lidoflazine. Biochem. Pharmacol. 48: 873-880.
19. Craig, J.E., Zhang, Y., and Gallagher, M.P. 1994. Cloning of the nupC gene of Escherichia coli encoding a nucleoside transport system, and identification of an adjacent insertion element, IS 186. Mol. Microbiol. 11: 1159-1168.
20. Crawford, C.R., Ng, C.Y., Noel, L.D., and Belt, J.A. 1990. Nucleoside transport in L1210 murine leukemia cells. Evidence for three transporters. J. Biol. Chem. 265: 9732-9736.
21. Crawford, C.R., Patel, D.H., and Belt, J.A. 1997. Isolation of a human cDNA that confers equilibrative, nitrobenzylmercaptopurine riboside-insensitive nucleoside transport activity (ei) to a transport deficient human leukemia cell line. Proc. Am. Assoc. Cancer Res. 38: A406.
22. Crawford, C.R., Patel, D.H., Naeve, C., and Belt, J.A. 1998a. Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transport-deficient cell line. J. Biol. Chem. 273: 5288-5293.
23. Crawford, C.R., Cass, C.E., Young, J.D., and Belt, J.A. 1998b. Stable expression of a recombinant sodium-dependent, pyrimidine-selective nucleoside transporter (rCNT1) in a transport-deficient mouse leukemia cell line. Biochem. Cell Biol. 76. This issue.
24. 3H)nitrobenzylthioinosine binding sites at the human parietal cortex and erythrocyte adenosine transporter: a comparison. Life Sci. 55: 1675-1682.
25. Fang, X., Parkinson, F.E., Mowles, D.A., Young, J.D., and Cass, C.E. 1996. Functional characterization of a recombinant sodium-dependent nucleoside transporter with selectivity for pyrimidine nucleosides (cNT1rat) by transient expression in cultured mammalian cells. Biochem. J. 317: 457-465.
26. +-dependent nucleoside transport in liver: two different isoforms from the same gene family are expressed in liver cells. Biochem. J. 330: 997-1001.
27. + dependent, guanosine specific, nitrobenzylthioinosine sensitive transporter in acute promyelocytic leukemia cells. J. Biol. Chem. 272: 18 026-18 032.
28. Gati, W.P., and Paterson, A.R.P. 1989. Nucleoside transport. In Red blood cell membranes: structure, function, clinical implications. Dekker, New York. pp. 635-661.
29. Geiger, D.D., and Fyda, D.M. 1991. Adenosine transport in nervous system tissues. Academic Press Ltd., New York. pp. 1-23.
30. Griffith, D.A., and Jarvis, S.M. 1996. Nucleoside and nucleobase transport systems of mammalian cells. Biochim. Biophys. Acta, 1286: 153-181.
31. Griffiths, M., Beaumont, N., Yao, S.Y., Sundaram, M., Boumah, C.E., Davies, A., Kwong, F.Y., Coe, I., Cass, C.E., Young, J.D., and Baldwin, S.A. 1997a. Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs. Nat. Med. 3: 89-93.
32. Griffiths, M., Yao, S.Y.M., Abidi, F., Phillips, S.E.V., Cass, C.E., Young, J.D., and Baldwin, S.A. 1997b. Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta. Biochem. J. 328: 739-743.
33. +-nucleoside transport in brush border membrane vesicles from human kidney. Biochim. Biophys. Acta, 1149: 202-208.
34. Gutierrez, M.M., Brett, C.M., Ott, R.J., Hui, A.C., and Giacomini, K.M. 1992. Nucleoside transport in brush border membrane vesicles from human kidney. Biochim. Biophys. Acta, 1105: 1-9.
35. +-dependent nucleoside transporter rCNT1. J. Physiol. (Lond.), 499: P50-P51.
36. Hogue, D.L., Ellison, M.J., Young, J.D., and Cass, C.E. 1996. Identification of a novel membrane transporter associated with intracellular membranes by phenotypic complementation in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 271: 9801-9808.
37. Huang, Q.Q., Harvey, C.M., Paterson, A.R., Cass, C.E., and Young, J.D. 1993. Functional expression of Na(+)-dependent nucleoside transport systems of rat intestine in isolated oocytes of Xenopus laevis. Demonstration that rat jejunum expresses the purine-selective system N1 (cif) and a second, novel system N3 having broad specificity for purine and pyrimidine nucleosides. J. Biol. Chem. 268: 20 613-20 619.
38. Huang, Q.Q., Yao, S.Y., Ritzel, M.W., Paterson, A.R., Cass, C.E., and Young, J.D. 1994. Cloning and functional expression of a complementary DNA encoding a mammalian nucleoside transport protein. J. Biol. Chem. 269: 17 757-17 760.
39. Jarvis, S.M. 1986. Nitrobenzylthioinosine-sensitive nucleoside transport system: mechanism of inhibition by dipyridamole. Mol. Pharmacol. 30: 659-665.
40. Jarvis, S.M., and Young, J.D. 1986. Nucleoside transport in rat erythrocytes: two components with differences in sensitivity to inhibition by nitrobenzylthioinosine and p-chloromercuriphenyl sulfonate. J. Membr. Biol. 93: 1-10.
41. Jarvis, S.M., McBride, D., and Young, J.D. 1982. Erythrocyte nucleoside transport: asymmetrical binding of nitrobenzylthioinosine to nucleoside permeation sites. J. Physiol. 324: 31-46.
42. Kwong, F.Y., Davies, A., Tse, C.M., Young, J.D., Henderson, P.J., and Baldwin, S.A. 1988. Purification of the human erythrocyte nucleoside transporter by immunoaffinity chromatography. Biochem. J. 255: 243-249.
43. Mackey, J.R., Baldwin, S.A., Young, J.D., and Cass, C.E. 1998a. Nucleoside transport and its significance for anticancer drug resistance. Drug Res. Updates, 1: 310-324.
44. Mackey, J.R., Mani, R.S., Selner, M., Mowles, D., Young, J.D., Belt, J.A., Crawford, C.R., and Cass, C.E. 1998b. Functional nucleoside transporters are required for gemcitabine and cytotoxicity in cancer cell lines. Cancer Res. 58: 4349-4357.
45. Mani, R.S., Hammond, J.R., Marjan, J.M.J., Graham, K.A., Young, J.D., Baldwin, S.A., and Cass, C.E. 1998. Demonstration of equilibrative nucleoside transporters (hENT1, hENT2) in intracellular membranes of cultured human choriocarcinoma (BeWo) cells by functional reconstitution in proteoliposome. J. Biol. Chem. 273: 30 818-30 825.
46. Osses, N., Pearson, J.D., Yudilevich, D.L., and Jarvis, S.M. 1996. Hypoxanthine enters human vascular endothelial cells (ECV 304) via the nitrobenzylthioinosine-insensitive equilibrative nucleoside transporter. Biochem. J. 317: 843-848.
47. +/nucleoside cotransporter. A member of the SGLT family. J. Biol. Chem. 267: 3557-3560.
48. Patel, D., Crawford, C., Naeve, C., and Belt, J. 1997. Molecular cloning and characterization of a mouse purine-selective concentrative nucleoside transporter. Proc. Am. Assoc. Cancer Res. 38: A405.
49. Paterson, A.R.P., and Cass, C.E. 1986. Transport of nucleoside drugs in animal cells. In Membrane transport of antineoplastic agents. Edited by I.D. Goldman. Pergamon Press, Oxford. pp. 309-329.
50. Paterson, A.R., Lau, E.Y., Dahlig, E., and Cass, C.E. 1980. A common basis for inhibition of nucleoside transport by dipyridamole and nitrobenzylthioinosine? Mol. Pharmacol. 18: 40-44.
51. +-linked transport of nucleoside analogs in leukemia cells from patients. Proc. Am. Assoc. Cancer Res. 34: A84.
52. Pisoni, R.L., and Thoene, J.G. 1989. Detection and characterization of a nucleoside transport system in human fibroblast lysosomes. J. Biol. Chem. 264: 4850-4856.
53. Pisoni, R.L., and Thoene, J.G. 1991. The transport systems of mammalian lysosomes. Biochim. Biophys. Acta, 1071: 351-373.
54. Plagemann, P.G., Wohlhueter, R.M., and Woffendin, C. 1988. Nucleoside and nucleobase transport in animal cells. Biochim. Biophys. Acta, 947: 405-443.
55. +-nucleoside cotransporter (hCNT1). Am. J. Physiol. 272: C707-C714.
56. +-nucleoside cotransporter (hCNT2) selective for purine nucleosides and uridine. Mol. Membr. Biol. 15: 203-211.
57. Schaner, M.E., Wang, J., Zevin, S., Gerstin, K.M., and Giacomini, K.M. 1997. Transient expression of a purine-selective nucleoside transporter (SPNT) in a human cell line (HeLa). Pharm. Res. 14: 1316-1321.
58. Sundaram, M., Yao, S.Y.M., Cass, C.E., Baldwin, S.A., and Young, J.D. 1998. Chimaeric constructs between human and rat equilibrative nucleoside transporters (hENT1 and rENT1) reveal hENT1 structural domains interacting with coronary vasoactive drugs. J. Biol. Chem. 273: 21 519-21 525.
59. Thorn, J.A., and Jarvis, S.M. 1996. Adenosine transporters. Gen. Pharmacol. 27: 613-620.
60. Vijayalakshmi, D., and Belt, J.A. 1988. Sodium-dependent nucleoside transport in mouse intestinal epithelial cells. Two transport systems with differing substrate specificities. J. Biol. Chem. 263: 19 419-19 423.
61. +-dependent nucleoside transporters. J. Biol. Chem. 272: 28 845-28 848.
62. +-dependent nucleoside transporters. Pharm. Res. 14: 1524-1532.
63. +-dependent purine nucleoside transporter from human kidney: cloning and functional characterization. Am. J. Physiol. 273: F1058-F1065.
64. Williams, J.B., and Lanahan, A.A. 1995. A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein. Biochem. Biophys. Res. Commun. 213: 325-333.
65. Wu, X., Yuan, G., Brett, C.M., Hui, A.C., and Giacomini, K.M. 1992. Sodium-dependent nucleoside transport in choroid plexus from rabbit. Evidence for a single transporter for purine and pyrimidine nucleosides. J. Biol. Chem. 267: 8813-8818.
66. Yao, S.Y., Cass, C.E., and Young, J.D. 1996a. Transport of the antiviral nucleoside analogs 3′-azido-3′-deoxythymidine and 2′,3′-dideoxycytidine by a recombinant nucleoside transporter (rCNT) expressed in Xenopus laevis oocytes. Mol. Pharmacol. 50: 388-393.
67. Yao, S.Y., Ng, A.M., Ritzel, M.W., Gati, W.P., Cass, C.E., and Young, J.D. 1996b. Transport of adenosine by recombinant purine-and pyrimidine-selective sodium/nucleoside cotransporters from rat jejunum expressed in Xenopus laevis oocytes. Mol. Pharmacol. 50: 1529-1535.
68. Yao, S.Y., Ng, A.M., Muzyka, W.R., Griffiths, M., Cass, C.E., Baldwin, S.A., and Young, J.D. 1997. Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues. J. Biol. Chem. 272: 28 423-28 430.