Engineering subtilisin YaB: Restriction of substrate specificity by the substitution of Gly24 and Gly151 with Ala

Protein Engineering

Volumn 11, Issue 2, 1998, Pages 109-117

  Mei, Hui Ching ^a   Liaw, Yen Chywan ^b   Li, Yao Cheng ^a   Wang, Der Chyun ^a   Takagi, Hiroshi ^c   Tsai, Ying Chieh ^a  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b INSTITUTE OF MOLECULAR BIOLOGY   (Taiwan)

^c FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Site directed mutagenesis; Structure modelling; Substrate specificity; Subtilisin YaB

Indexed keywords

ALANINE; AMINO ACID DERIVATIVE; ANILIDE; CASEIN; ELASTIN; GLYCINE; MUSCLE PROTEIN; MUTANT PROTEIN; SUBTILISIN; THERMITASE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; HYDROLYSIS; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

ALANINE; AMINO ACID SEQUENCE; COMPUTER SIMULATION; CRYSTALLIZATION; GENE EXPRESSION; GLYCINE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; SUBTILISINS;

BACILLUS SUBTILIS; FELIS CATUS;

EID: 0031941034     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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