Construction of novel subtilisin E with high specificity, activity and productivity through multiple amino acid substitutions

Protein Engineering

Volumn 10, Issue 9, 1997, Pages 985-989

  Takagi, Hiroshi ^a   Ohtsu, Iwao ^a   Nakamori, Shigeru ^a  

^a FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Autoprocessing; Catalytic efficiency; Site directed mutagenesis; Substrate specificity; Subtilisin

Indexed keywords

ALANINE; ASPARTIC ACID; GLYCINE; ISOLEUCINE; LEUCINE; MUTANT PROTEIN; SUBTILISIN; TYROSINE; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; STEREOSPECIFICITY;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); FELIS CATUS; POSIBACTERIA;

EID: 0031449410     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.9.985     Document Type: Article

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