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21. Thoden JB, Miran SG, Phillips JC, Howard AJ, Raushel FM, Holden HM: Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis. Biochemistry 1998, 37:8825-8831. This paper describes the structural analysis of a site-directed mutant of CPS, in which a glutamyl thioester intermediate has been trapped in the active site of the small subunit. This investigation provides the first direct structural observation of an enzyme intermediate in the amidotransferase family.
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