A side chain in S6 influences both open-state stability and ion permeation in a voltage-gated K+ channel

Pflugers Archiv European Journal of Physiology

Volumn 435, Issue 5, 1998, Pages 654-661

  Liu, Yu ^a   Joho, Rolf H ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Channel mutants; Gating; Ion selectivity; Pore; Potassium channel

Indexed keywords

POTASSIUM CHANNEL; TETRYLAMMONIUM;

ANIMAL CELL; ARTICLE; CHANNEL GATING; CONFORMATIONAL TRANSITION; ION CONDUCTANCE; ION TRANSPORT; MUTANT; NONHUMAN; POTASSIUM CONDUCTANCE; PRIORITY JOURNAL; XENOPUS LAEVIS;

AMINO ACID SUBSTITUTION; ANIMALS; CYSTEINE; DELAYED RECTIFIER POTASSIUM CHANNELS; ELECTRIC CONDUCTIVITY; ION CHANNEL GATING; MUTATION; OOCYTES; PATCH-CLAMP TECHNIQUES; PERMEABILITY; POTASSIUM CHANNEL BLOCKERS; POTASSIUM CHANNELS; POTASSIUM CHANNELS, VOLTAGE-GATED; SHAB POTASSIUM CHANNELS; TETRAETHYLAMMONIUM; XENOPUS LAEVIS;

EID: 0031933383     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240050566     Document Type: Article

References (46)

1. Aiyar J, Nguyen AA, Chandy KG, Grissmer S (1994) The P-region and S6 of Kv3.1 contribute to the formation of the ion conduction pathway. Biophys J 67:2261-2264
2. + channel revealed by the NMR-derived structures of scorpion toxins. Neuron 15:1169-1181
3. Aiyar J, Rizzi JP, Gutman GA, Chandy KG (1996) The signature sequence of voltage-gated potassium channels projects into the external vestibule. J Biol Chem 271:31013-31016
4. Armstrong CM (1971) Interaction of tetraethylammonium ion derivatives with the potassium channels of giant axon. J Gen Physiol 58:413-437
5. Armstrong CM, Swenson RP, Taylor SR (1982) Block of squid axon channels by internally and externally applied barium ions. J Gen Physiol 80:663-682
6. Bezanilla F, Perozo E, Papazian DM, Stefani E (1991) Molecular basis of gating charge immobilization in Shaker potassium channels. Science 254:679-683
7. + channels: II. The components of gating currents and a model of channel activation. Biophys J 66:1011-1021
8. Choi KL, Mossman C, Aubé J, Yellen G (1993) The internal quaternary ammonium receptor site of Shaker potassium channels. Neuron 10:533-541
9. Creighton TE (1984) Proteins: structures and molecular properties. Freeman, New York
10. + channel. Neuropharmacology 35:761-773
11. Frech GC, VanDongen AMJ, Schuster G, Brown AM, Joho RH (1989) A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning. Nature 340:642-645
12. Grissmer S, Cahalan MD (1989) Divalent ion trapping inside potassium channels of human lymphocytes. J Gen Physiol 93: 609-630
13. + channels. Science 251:942-944
14. + channel signature sequence. Biophys J 66: 1061-1067
15. + channel pore through mutant cycles with a peptide inhibitor. Science 268:307-310
16. Hille B (1992) Ionic channels of excitable membranes. Sinauer, Sunderland, Mass.
17. + channels. Evidence for a trap door mechanism of activation gating. J Gen Physiol 109:527-535
18. Hoshi T, Zagotta WN, Aldrich RW (1994) Shaker potassium channel gating I: transitions near the open state. J Gen Physiol 103:249-278
19. Karpusas M, Baase WA, Matsumara M, Matthews BW (1989) Hydrophobic packing in T4 lysozyme probed by cavity-filling mutants. Proc Natl Acad Sci USA 86:8237-8241
20. + channels determined by an interacting pair of amino acids. Neuron 8:499-505
21. + conductance switch. Biophys J 62:136-144
22. Korn SJ, Ikeda SR (1995) Permeation selectivity by competition in a delayed rectifier potassium channel. Science 269: 410-412
23. + channel pore. Biophys J 72: 117-126
24. + channel probed by sulfhydryl-specific reagents after cysteine-scanning mutagenesis. Biophys J 68:900-905
25. + channel. Neuron 19:175-184
26. Lo DC, Pinkham JL, Stevens CF (1991) Role of a key cysteine residue in the gating of the acetylcholine receptor. Neuron 6: 31-40
27. + channel comprises part of the pore. Nature 367:179-182
28. Lu Q, Miller C (1995) Silver as a probe of pore-forming residues in a potassium channel. Science 268:304-307
29. Mannuzzu LM, Moronne MM, Isacoff EY (1996) Direct physical measure of conformational rearrangement underlying potassium channel gating. Science 271:213-216
30. + channel. J Gen Physiol 90:427-449
31. Moorman JR, Kirsch GE, VanDongen AMJ, Joho RH, Brown AM (1990) Fast and slow gating of sodium channels encoded by a single mRNA. Neuron 4:243-252
32. Ogielska EM, Aldrich RW (1997) A single amino acid substitution in the S6 of Shaker decreases potassium affinity and allows for sodium permeation in the absence of potassium. Biophys J 72:A233
33. + pore structure revealed by reporter cysteines at inner and outer surfaces. Neuron 14:1055-1063
34. Perozo E, Santacruz-Toloza L, Stefani E, Bezanilla F, Papazian DM (1994) S4 mutations alter gating currents of Shaker K channels. Biophys J 66:345-354
35. + channel selectivity filter by mutant cycle-based structure analysis. Neuron 16:131-139
36. Schoppa NE, McCormack K, Tanouye MA, Sigworth FJ (1992) The size of gating charge in wild-type and mutant Shaker potassium channels. Science 255:1712-1715
37. Slesinger PA, Jan YN, Jan LY (1993) The S4-S5 loop contributes to the ion-selective pore of potassium channels. Neuron 11:739-749
38. + channels: I. Ionic and gating currents. Biophys J 66:996-1010
39. + channels. Mol Pharmacol 40:299-307
40. + channel function by deletions at the N- and C-termini. Neuron 5:433-443
41. + channel. Science 251: 939-942
42. + channel by mutation of the H5 region. Nature 349: 700-704
43. Zagotta WN, Hoshi T, Dittman J, Aldrich RW (1994) Shaker potassium channel gating II: transitions in the activation pathway. J Gen Physiol 103:279-319
44. + channel in a nonconducting state. Biophys J 71:3083-3090
45. + channel Kv2.1. Receptors Channels 2:237-248
46. Zühlke RD, Zhang H-J, Joho RH (1995) In: Conn PM (ed) Methods in Neurosciences, vol. 25. Academic, New York, pp 67-89