Molecular cloning and expression of the spsB gene encoding an essential type I signal peptidase from Staphylococcus aureus

Journal of Bacteriology

Volumn 178, Issue 19, 1996, Pages 5712-5718

  Cregg, Karen M ^a   Wilding, E Imogen ^a   Black, Michael T ^a,b  

^a GLAXOSMITHKLINE   (United Kingdom)

^b SmithKline Beccham Pharmaceuticals   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PEPTIDASE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; DNA DETERMINATION; DNA FLANKING REGION; GENE EXPRESSION; GENETIC CODE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SOUTHERN BLOTTING; STAPHYLOCOCCUS AUREUS;

EID: 0029796449     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.19.5712-5718.1996     Document Type: Article

References (44)

1. Akagawa, E., K. Kurita, T. Sugawara, K. Nakamura, Y. Kasahara, N. Ogasawara, and K. Yamane. 1995. Determination of a 17848 bp nucleotide sequence around the 39° region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs. Microbiology 141:3241-3245.
2. Allsop, A. E., G. Brooks, G. Bruton, S. Coulton, P. D. Edwards, I. K. Hatton, A. C. Kaura, S. U. McLean, N. D. Pearson, T. Smale, and R. Southgate. 1995. Penem inhibitors of bacterial signal peptidase. Bioorg. Med. Chem. Lett. 5:443-448.
3. Black, M. T. 1993. Evidence that the catalytic activity of prokaryote leader peptidase depends upon the operation of a serine-lysine catalytic dyad. J. Bacteriol, 175:4957-4961.
4. Black, M. T., J. G. R. Munn, and A. E. Allsop. 1992. On the catalytic mechanism of prokaryotic leader peptidase I. Biochem. J. 282:539-543.
5. 4a. Cregg, K. M., and M. T. Black. Unpublished data.
6. Dalbey, K. E., and W. Wickner. 1985. Leader peptidase catalyzes the release of exported proteins from the outer surface of the Escherichia coli plasma membrane. J. Biol. Chem. 260:15925-15931.
7. Date, T. 1983. Demonstration by a novel genetic technique that leader peptidase is an essential enzyme of Escherichia coli J. Bacteriol. 154:76-83.
8. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
9. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J.-F. Tomb, B. A. Dougherty, J. M. Merrick, K. McKenney, G. Sutton, W. FitzHugh, C. Fields, J. D. Gocayne, J. Scott, R. Shirley, L.-I, Liu, A. Glodek, J. M. Kelly, J. F. Weldman, C. A. Phillips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Utterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritchman, J. L. Fuhrmann, N. S. M. Geoghagen, C. L. Gnehm, L. A. McDonald, K. V. Small, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
10. Fraser, C. M., J. D. Gocayne, O. White, M. D. Adams, R. A. Clayton, R. D. Fleischmann, C. J. Bult, A. R. Kerlavage, G. Sutton, J. M. Kelley, J. L. Fritchman, J. F. Weidman, K. V. Small, M. Sandusky, J. Fuhrmann, D. Nguyen, T. R. Utterback, D. M. Saudek, C. A. Phillips, J. M. Merrick, J.-F. Tomb, B. A. Dougherty, K. F. Bott, P.-C. Hu, T. S. Lucier, S. C. Peterson, H. O. Smith, C. A. Hutchison III, and J. C. Venter. 1995. The minimal gene complement of Mycoplasma genitalium. Science 270:397-403.
11. Hamilton, C. M., M. Aldea, B. K. Washburn, P. Babitzke, and S. R. Kushner. 1989. New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171:4617-4622.
12. Hoang, V., A. Birger, and J. Hofemeister. 1993. Sequence P42668. Submitted to EMBL/GenBank/DDBJ data banks.
13. Hoang, V., and J. Hofemeister. 1995. B. amyloliquefaciens possesses a second type I signal peptidase with extensive sequence similarity to other Bacillus SPases, Biochim. Biophys. Acta 1269:64-68.
14. Hojrup, P., M. S. Jensen, and T. E. Petersen. 1995. Amino acid sequence of bovine protein Z: a vitamin K-dependent serine protease homolog. FEBS Lett. 184:333-378.
15. Inada, T., D. L. Court, K. Ito, and Y. Nakamura. 1988. Conditionally lethal amber mutations in the leader peptidase gene of Escherichia coli. J. Bacteriol. 171:585-587.
16. Jacob, F., B. Joris, and J.-M. Frère. 1991. Active site serine mutants of the Streptomyces albus G β-lactamase. Biochem. J. 277:647-652.
17. Keiler, K. C., and R. T. Sauer. 1995. Identification of active site residues of the Tsp protcase, J. Biol. Chem. 270:28864-28868.
18. Klug, G., A. Jaeger, C. Heck, and R. Rauhut. 1996. GenBank accession number Z68305.
19. Kurosky, A., D. R. Barnett, T.-H. Lee, B. Touchstone, R. E. Hay, M. S. Arnott, B. H. Bowman, and W. M. Fitch. 1980. Covalent structure of human haptoglobin: a serine protease homolog. Proc. Natl. Acad. Sci. USA 77: 3388-3392.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
21. Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208-218.
22. Meijer, W. J. J., A. de Jong, G. Bea, A. Wiseman, H. Tjalsma, G. Venema, S. Bron, and J. M. van Dijl. 1995 The endogenous R. subtilis (natto) plasmids pTA1015 and pTA1040 contain signal peptidase-encoding genes: identification of a new structural module un cryptic plasmids. Mol. Microbiol. 17:621-631.
23. Müller, P., K. Ahrens, and A. Klaucke. 1995. A TnphoA insertion within the Bradyrhizobium japonicum sipS gene, homologous to prokaryotic signal peptidases, results in extensive changes in the expression of PMB-specific nodulins of infected soybean (Glycine max) cells. Mol. Microbiol. 18:831-840.
24. Müller, P., A. Klaucke, and E. Wegel. 1995. TnphoA-induced symbiotic mutants of Bradyrhizobium japonicum that impair cell and tissue differentiation in Glycine max nodules. Planta 197:163-175.
25. Nakamura, T., T. Nishizawa, M. Hagiya, T. Seki, M. Shimonishi, A. Sugimura, K. Tashire, and S. Shimizu. 1989. Molecular cloning and expression of human hepatocyte growth factor. Nature (London) 342:440-443.
26. Packer, J. C., D. Andre, and C. J. Howe. 1995. Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum. Plant Mol. Biol. 27:199-204.
27. Petersen, L. C., J. J. Birktoft, and H. Flodgaard. 1993. Binding of bovine pancreatic trypsin inhibitor to heparin binding protein/CAP37/azurocidin. Interaction between a Kunitz-type inhibitor and a proteolytically inactive serine proteinase homologue. Eur. J. Biochem. 214:271-279.
28. Rifai, S., V. Barbancon, G. Prevost, and Y. Piemont. 1989. Synthetic exfoliative toxin A and B DNA probes for detection of toxigenic Staphylococcus aureus strains. J. Clin. Microbiol. 27:504-506.
29. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
30. Schenk, S., and R. A. Laddaga. 1992. Improved method for electroporation of Staphylococcus aureus. FEMS Microbiol. Lett. 94:133-138.
31. Slilaty, S. N., and J. Little. 1987. Lysine-156 and serine-119 are required for LexA repressor cleavage: a possible mechanism. Proc. Natl. Acad. Sci. USA 84:3987-3991.
32. Sung, M., and R. E. Dalbey. 1992. Identification of potential active-site residues in the Escherichia coli leader peptidase. J. Biol. Chem. 267:13154-13159.
33. Toth, M. J., E. J. Murgola, and P. Schimmel. 1988. Evidence for a unique first position codon-anticodon mismatch in vivo. J. Mol. Biol. 201:451-454.
34. Tschantz, W. R., M. Sung, V. M. Delgado-Partin, and R. E. Dalbey. 1993. A serine and a lysine residue implicated in the catalytic mechanism of the Escherichia coli leader peptidase. J. Biol. Chem. 268:27349-27354.
35. van Dijl, J. M. 1993. Sequence p41027. Submitted to EMBL/GenBank/DDBJ data banks.
36. van DiJl, J. M. 1993. Sequence p41026. Submitted to EMBL/GenBank/DDBJ data banks.
37. van Dijl, J. M., A. de Jong, H. Smith, S. Bron, and G. Venema. 1991. Non-functional expression of Escherichia coli signal peptidase I in B. subtilis. J. Gen. Microbiol. 137:2073-2083.
38. van Dijl, J. M., A. de Jong, J. Vehmaanpera, G. Venema, and S. Bron. 1992. Signal peptidase I of B. subtilis: patterns of conserved amino acids in prokaryotic and eukaryotic type 1 signal peptidases. EMBO J. 11:2819-2828.
39. van Dijl, J. M., A. de Jong, G. Venema, and S. Bron. 1995. Identification of the potential active site of the signal peptidase SipS of B. subtilis. J. Biol. Chem. 270:3611-3618.
40. van Dijl, J. M., R. van den Bergh, T. Reversma, H. Smith, S. Bron, and G. Venema. 1990. Molecular cloning of the Salmonella typhimurium lep gene in Escherichia coli. Mol. Gen. Genet. 223:233-240.
41. Vanklompenberg, W., P. Whitley, R. Diemel, G. von Heijne, and B. deKruijff. 1995. A quantitative assay to determine the amount of signal peptidase-1 in Escherichia coli and the orientation of membrane vesicles. Mol. Membr. Biol. 12:349-353.
42. Villafane, R., D. H. Bechhofer, C. S. Narayanan, and D. Dubnau. 1987. Replication control genes of plasmid pE194. J. Bacteriol. 169:4822-4829.
43. Wickner, W., A. J. M. Driessen, and F.-U. Hartl. 1991. The enzymology of protein translocation across the Escherichia coli plasma membrane. Annu. Rev. Biochem. 60:101-124.
44. Wolfe, P. B., W. Wickner, and J. M. Goodman. 1983. Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope. J. Biol. Chem. 258:12073-12080.