The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity

RNA

Volumn 4, Issue 7, 1998, Pages 856-869

  Motorin, Y ^a   Keith, G ^b   Simon, C ^a   Foiret, D ^a   Simos, G ^c   Hurt, E ^c   Grosjean, H ^a  

^a LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

^b INST DE BIOL MOLEC ET CELLULAIRE   (France)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Intron; Maturation; Pseudouridine synthase; Saccharomyces cerevisiae; tRNA; Yeast

Indexed keywords

SYNTHETASE; TRANSFER RNA; URIDINE DERIVATIVE;

ARTICLE; CATALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; INTRON; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; RNA SEQUENCE; SACCHAROMYCES CEREVISIAE; YEAST;

CLONING, MOLECULAR; FUNGAL PROTEINS; HYDRO-LYASES; MUTATION; PSEUDOURIDINE; RECOMBINANT PROTEINS; RNA PRECURSORS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, FUNGAL; RNA, PLANT; RNA, TRANSFER; SACCHAROMYCES CEREVISIAE; SUBCELLULAR FRACTIONS; SUBSTRATE SPECIFICITY;

SACCHAROMYCES CEREVISIAE;

EID: 0031870753     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838298980396     Document Type: Article

References (47)

1. Tyr introns: Their evolution from red algae to higher plants. FEBS Lett 417:213-218.
2. Arluison V, Hountoundji C, Robert B, Grosjean H. 1998. Transfer RNA:pseudouridine synthase Pus1 of S. cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition. Biochemistry 37:7268-7276.
3. Arts GJ, Foprnerod M, Mattaj I. 1998. Identification of a nuclear export receptor for tRNA. Curr Biol 8:305-314.
4. Auxilien S, Crain PF, Trewyn RW, Grosjean H. 1996. Mechanism, specificity and general properties of the yeast enzyme catalysing the formation of inosine 34 in the anticodon of transfer RNA. J Mol Biol 262:437-458.
5. Bakin A, Kowalak JA, McCloskey JA, Ofengand J. 1994. The single pseudouridine residue in Escherichia coli 16S RNA is located at position 516. Nucleic Acids Res 22:3681-3684.
6. Bakin A, Ofengand J. 1993. Four newly located pseudouridylate residues in Escherichia coli 23S ribosomal RNA are all at the peptidyltransferase center - Analysis by the application of a new sequencing technique. Biochemistry 32:9754-9762.
7. Bakin A, Ofengand J. 1995. Mapping of the 13 pseudouridine residues in Saccharomyces cerevisiae small subunit ribosomal RNA to nucleotide resolution. Nucleic Acids Res 23:3290-3294.
8. Becker HF, Motorin Y, Planta RJ, Grosjean H. 1997a. The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of Ψ55 in both mitochondrial and cytoplasmic tRNAs. Nucleic Acids Res 25:4493-4499.
9. Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H. 1997b. Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the TΨ-loop of yeast tRNAs. J Mol Biol 274:505-518.
10. Bousquet-Antonelli C, Henry Y, Gelugne JP, Caizergues-Ferrer M, Kiss T. 1997. A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs. EMBO J 16:4770-4776.
11. Foster PG, Huang L, Santi DV, Stroud RM. 1997. The crystal structure of E. coli tRNA pseudouridine synthase I. FASEB J 11:A862.
12. Fradin A, Gruhl H, Feldmann H. 1975. Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels. FEBS Lett 50:185-189.
13. Ganot P, Bortolin ML, Kiss T. 1997. Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs. Cell 89:799-809.
14. Grosjean H, Szweykowska-Kulinska Z, Motorin Y, Fasiolo F, Simos G. 1997. Intron-dependent enzymatic formation of modified nucleosides in eukaryotic tRNAs: A review. Biochimie 79:293-302.
15. Gu X, Yu M, Ivanetich KM, Santi DV. 1998. Molecular recognition of tRNA by tRNA pseudouridine 55 synthase. Biochemistry 37:339-343.
16. Gustafsson C, Reid R, Greene PJ, Santi DV. 1996. Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes. Nucleic Acids Res 24:3756-3762.
17. Heitzler J, Maréchal-Drouard L, Dirheimer G, Keith G. 1992. Use of a dot blot hybridization method for identification of pure tRNA species on different membranes. Biochim Biophys Acta 1129:273-277.
18. Hellmuth K, Lau D, Bischoff R, Künzler M, Hurt E, Simos G. 1998. Evidence for a function of Los1p as a tRNA nuclear export receptor. Mol Cell Biol. Forthcoming.
19. Huang L, Pookanjanatavip M, Gu X, Santi DV. 1998. A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst. Biochemistry 37:344-351.
20. Phe: An in vitro study. Nucleic Acids Res 25:2694-2701.
21. Tyr gene intron is essential for correct modification of its tRNA product. Nature 302:681-687.
22. Kammen HO, Marvel CC, Hardy L, Penhoet EE. 1988. Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I. J Biol Chem 263:2255-2263.
23. Keith G. 1990. Nucleic acid chromatographic isolation and sequence methods. In: Gehrke CW, Kuo KC, eds. Chromatography and modifications of nucleosides. Chromatography Library Series, vol. 45A. Amsterdam, The Netherlands: Elsevier. ppA103-A141.
24. Koonin EV. 1996. Pseudouridine synthases: Four families of enzymes containing a putative uridine-binding motif also conserved in dUTPases and dCTP deaminases. Nucleic Acids Res 24:2411-2415.
25. Kutay U, Lipowsky G, Izaurralde E, Bischoff F, Schwarzmeier P, Hartmann E, Görlich D. 1998. Identification of a tRNA-specific nuclear export receptor. Mol Cell 1:359-369.
26. Lafontaine DLJ, Bousquet-Antonelli C, Henry Y, Caizergues-Ferrer M, Tollervey D. 1998. The box H+ACA snoRNAs carry the putative rRNA pseudouridine synthase. Genes & Dev 12:527-537.
27. 39 in tRNA anticodon loop. J Biol Chem 273:1316-1323.
28. Massenet S, Mougin A, Branlant C. 1998. Posttranscriptional modifications in the U small nuclear RNAs. In: Grosjean H, Benne R, eds. The modification and editing of RNA. New York: ASM Press. pp 201-227.
29. Monier R, Stephenson ML, Zamecnik PC. 1960. The preparation and some properties of a low molecular weight ribonucleic acid from baker's yeast. Biochim Biophys Acta 43:1-8.
30. Ni JW, Tien AL, Fournier MJ. 1997. Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA. Cell 89:565-573.
31. Nurse K, Wrzesinski J, Bakin A, Lane BG, Ofengand J. 1995. Purification, cloning, and properties of the tRNA Ψ55 synthase from Escherichia coli. RNA 1:102-112.
32. Ofengand J, Fournier M. 1998. The pseudouridine residues of rRNA: Number, location, biosynthesis, and function. In: Grosjean, H, Benne R, eds. The modification and editing of RNA. New York: ASM Press, pp 229-253.
33. Asp transcripts deprived of modified nucleotides. Biochimie 72:735-744.
34. Sampson J, DiRenzo AB, Behlen LS, Uhlenbeck OC. 1990. Role of the tertiary nucleotides in the interaction of yeast phenylalanine tRNA with its cognate synthetase. Biochemistry 29:2523-2532.
35. Samuelsson T, Olsson M. 1990. Transfer RNA pseudouridine synthases in Saccharomyces cerevisiae. J Biol Chem 265:8782-8787.
36. Senger B, Despons L, Walter P, Fasiolo F. 1992. The anticodon triplet is not sufficient to confer methionine acceptance to a transfer RNA. Proc Natl Acad Sci USA 89:10768-10771.
37. Shen WC, Selvakumar D, Stanford D, Hopper A. 1993. The Saccharomyces cerevisiae LOS1 gene involved in pre-tRNA splicing encodes a nuclear protein that behaves as a component of the nuclear matrix. J Biol Chem 268:19436-19444.
38. Simos G, Sauer A, Fasiolo F, Hurt EC. 1998. A conserved domain within Arc1p delivers tRNA to aminoacyl-tRNAsynthetases. Mol Cell 1:235-242.
39. Simos G, Tekotte H, Grosjean H, Segref A, Sharma K, Tollervey D, Hurt EC. 1996. Nuclear pore proteins are involved in the biogenesis of functional tRNA. EMBO J 15:2270-2284.
40. Smith CM, Steitz JA. 1997. Sno storm in the nucleolus: New roles for myriad small RNPs. Cell 59:699-672.
41. Sprinzl S, Horn C, Brown M, Ioudovitch A, Steinberg S. 1998. Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res 26:148-153.
42. Swerdlow H, Guthrie C. 1984. Structure of intron-containing tRNA precursors. Analysis of solution conformation using chemical and enzymatic probes. J Biol Chem 259:5197-5207.
43. Tyr. EMBO J 11:1907-1912.
44. Tyr affecting pseudouridylation of U35. Biochim Biophys Acta 1264:87-92.
45. lle. EMBO J 13:4636-4644.
46. Wilhelm ML, Baranovski W, Keith G, Wilhelm FX. 1992. Rapid transfer of small RNAs from a polyacrylamide gel onto a nylon membrane using a gel dryer. Nucleic Acids Res 20:4106.
47. phe. RNA 1:437-448.