Escherichia coli cytotoxic necrotizing factor 1 effaces microvilli and decreases transmigration of polymorphonuclear leukocytes in intestinal T84 epithelial cell monolayers

Infection and Immunity

Volumn 66, Issue 6, 1998, Pages 2494-2500

  Hofman, Paul ^a   Flatau, Gilles ^a   Selva, Eric ^a   Gauthier, Michel ^a   Le Negrate, Gaëlle ^a   Fiorentini, Carla ^b   Rossi, Bernard ^a   Boquet, Patrice ^a  

^a INSERM   (France)

^b ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN; CYTOTOXIC NECROTIZING FACTOR 1; F ACTIN; FORMYLMETHIONYLLEUCYLPHENYLALANINE; GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ARTICLE; CELL MIGRATION; CONTROLLED STUDY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; INTESTINE BRUSH BORDER; INTESTINE EPITHELIUM CELL; MEMBRANE RESISTANCE; MONOLAYER CULTURE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0031841356     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.6.2494-2500.1998     Document Type: Article

References (40)

1. Adam, T., M. Giry, P. Baquet, and P. Sansonetti. 1996. Rho-dependent membrane folding causes Shigella entry into cells. EMBO J. 15:3315-3321.
2. 1a.Boquet, P., et al. Unpublished data.
3. Braga, V. M. M., L. M. Machesky, A. Hall, and N. A. Hotchin. 1997. The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts. J. Cell Biol. 137:1421-1431.
4. Caprioli, A., V. Falbo, L. G. Roda, F. M. Ruggeri, and C. Zona. 1983. Partial purification and characterization of an Escherichia coli toxic factor that induces morphological alterations. Infect. Immun. 39:1300-1306.
5. Chardin, P. 1993. Structural conservation of Ras-relatcd proteins and its functional implications, p. 159-176. In B. F. Dickey and L. Birnbaumer (ed.), Handbook of experimental pharmacology, GTPases in biology. Springer Verlag, Heidelberg, Germany.
6. Dharmasathaphorn, K., J. A. McRobert, K. G. Mandel, L. D. Tisdale, and H. Masui. 1984. A human colonie tumor cell that maintains vectorial electrolyte transport. Am. J. Physiol. 246:6204-6208.
7. Donnenberg, M. S., and J. B. Kaper. 1992. Enteropathogenic Escherichia coli. Infect. Immun. 60:3953-3961.
8. Eichel-Streiber, C., P. Boquet, M. Sauerborn, and M. Thelestam. 1996. Large clostridial toxins, a family of glycosyl transferases modifying small GTP-binding proteins. Trends Microbiol. 4:375-382.
9. Falzano, L., C. Fiorentini, G. Donelli, E. Michel, C. Kocks, P. Cossart, L. Cabanié, E. Oswald, and P. Boquet. 1993. Induction of phagocytic behaviour in human epithelial cells by Escherichia coli cytotoxic necrotizing factor type 1. Mol. Microbiol. 9:1247-1254.
10. Finlay, B., and P. Cossart. 1997. Exploitation of mammalian cell functions by bacterial pathogens. Science 276:718-725.
11. Fiorentini, C., G. Arancia, A. Caprioli, V. Falbo, F. M. Ruggeri, and G. Donelli. 1988. Cytoskeletal changes induced in Hep-2 cells by the cytotoxic necrotizing factor of Escherichia coli. Toxicon 26:1047-1056.
12. Fiorentini, C., G. Donelli, P. Matarrese, A. Fabbri, S. Paradisi, and P. Boquet. 1995. Escherichia coli cytotoxic necrotizing factor 1: evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase. Infect. Immun. 63:3936-3944.
13. Flatau, G., E. Lemichez, M. Gauthier, P. Chardin, S. Paris, C. Fiorentini, and P. Boquet. 1997. Toxin-induced activation of p21 Rho by glutamine deamidation. Nature 387:729-733.
14. Galan, J. E., and J. B. Bliska. 1996. Cross-talk between bacterial pathogens and their host cells. Annu. Rev. Cell Dev. Biol. 12:221-255.
15. 2+ sensitization of smooth muscle. Proc. Natl. Acad. Sci. USA 93:1340-1345.
16. Hofman, P., L. D'Andrea, D. Carnes, S. P. Colgan, and J. L. Madara. 1996. Intestinal epithelial cytoskeleton selectively constrains lumen-to-tissue migration of neutrophils. Am. J. Physiol. 271:312-320.
17. Kimura, K., M. Ito, M. Amano, K. Chihara, Y. Fukata, M. Nakafuku, B. Yamamori, J. Feng, T. Nakano, K. Okawa, A. Iwamatsu, and K. Kaibuchi. 1996. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273:245-247.
18. Machesky, L. M., and A. Hall. 1996. Rho: a connection between membrane receptor signalling and the cytoskeleton. Trends Cell Biol. 6:304-310.
19. Madara, J. L., D. Barenberg, and S. Carlson. 1986. Effects of cytochalasin D on occluding junctions of intestinal absortive cells: further evidence that the cytoskeleton may influence paracellular permeability. J. Cell Biol. 102:2125-2135.
20. Madara, J. L. 1987. Intestinal absortive cell tight junctions are linked to cytoskeleton. Am. J. Physiol. 253:171-175.
21. Madara, J. L., R. Moore, and S. Carlson. 1987. Alteration of intestinal tight junction structure and permeability by cytoskeletal contraction. Am. J. Physiol. 253:854-861.
22. Madara, J. L., J. Stafford, D. Barenberg, and S. Carlson. 1988. Functional coupling of tight junctions and microfilaments in T84 monolayers. Am. J. Physiol. 254:416-423.
23. Madara, J. L. 1989. Loosening tight junctions. Lessons from the intestine. J. Clin. Invest. 83:1089-1094.
24. Madara, J. L., S. Colgan, A. Nusrat, C. Delp, and C. Parkos. 1992. A simple approach to measurement of electrical parameters of cultured epithelial monolayers: use in assessing neutrophil epithelial monolayers transmigration. J. Tissue Culture Methods 14:209-216.
25. Mecsas, J., and E. J. Strauss. 1996. Molecular mechanisms of bacterial virulence-type III secretion and pathogenicity islands. Emerging Infect. Dis. 2:271-288.
26. - secretion. Am. J. Physiol. 258:165-172.
27. Nash, S., J. Stafford, and J. L. Madara. 1987. Effects of polymorphonuclear leukocyte transmigration on the barrier function of cultured intestinal epithelial monolayers. J. Clin. Invest. 80:1104-1113.
28. Nobes, C. D., and A. Hall. 1995. Rho, Rac and Cdc42 GTPases regulate the assembly of multimolecular complexes associated with actin stress fibers, lamellipodia and filopodia. Cell 81:53-62.
29. Nusrat, A., M. Giry, J. R. Turner, S. P. Colgan, C. A. Parkos, E. Lemichez, P. Boquet, and J. L. Madara. 1995. Rho protein regulates tight junctions and perijunctional actin organization in polarized epithelia. Proc. Natl. Acad. Sci. USA 92:10629-10633.
30. Parkos, C. P., C. Delp, M. A. Arnaout, and J. L. Madara. 1991. Neutrophil migration across a cultured intestinal epithelium. Dependence on a CD11b/CD18-mediated event and enhanced efficiency in physiological direction. J. Clin. Invest. 88:1605-1612.
31. Parkos, C. A., S. P. Colgan, T. W. Liang, A. Nusrat, A. E. Bacarra, D. K. Carnes, and J. L. Madara. 1996. CD47 mediates post-adhesive events required for neutrophil migration across polarized intestinal epithelia. J. Cell Biol. 132:437-450.
32. Price, A. B., and D. R. Davies. 1977. Pseudomembranous colitis. J. Clin. Pathol. 30:1-12.
33. Ridley, A. J., and A. Hall. 1992. The small GTP-binding protein Rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70:389-400.
34. Ridley, A. J., H. F. Paterson, C. L. Johnston, D. Diekmann, and A. Hall. 1992. The small GTP-binding protein Rac regulates growth factor-induced membrane ruffling. Cell 70:401-410.
35. Salmond, G. P. C. 1994. Secretion of extracellular virulence factors by plant pathogenic bacteria. Annu. Rev. Physiopathol. 32:181-200.
36. Schmidt, G., P. Sehr, M. Wilm, J. Selzer, M. Mann, and K. Aktories. 1997. Gln63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1. Nature 387:725-728.
37. ++: a protein kinase C effect. J. Membr. Biol. 149:71-79.
38. Takaishi, K., T. Sasaki, H. Kotani, H. Nishioka, and Y. Takai. 1997. Regulation of cell-cell adhesion by Rac and Rho small G proteins in MDCK cells. J. Cell Biol. 139:1047-1059.
39. Watarai, M., Y. Kamata, S. Kosaki, and C. Sasakawa. 1997. Rho, a small GTP-binding protein, is essential for Shigella invasion of epithelial cells. J. Exp. Med. 185:281-292.
40. Yardley, J. H. 1986. Pathology of idiopathic inflammatory bowel disease and relevance of specific cell findings: an overview p. 3-9. In Recent developments in the therapy of inflammatory bowel disease. Proceedings of a symposium. Johns Hopkins University, Baltimore, Md.