Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells

Journal of Clinical Investigation

Volumn 97, Issue 7, 1996, Pages 1647-1654

  Mashima, Hirosato ^a   Ohnishi, Hirohide ^a   Wakabayashi, Katsumi ^a   Mine, Tetsuya ^b   Miyagawa, Jun Ichiro ^c   Hanafusa, Toshiaki ^c   Seno, Masaharu ^d   Yamada, Hidenori ^d   Kojima, Itaru ^a  

^a GUNMA UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c OSAKA UNIVERSITY MEDICAL SCHOOL   (Japan)

^d OKAYAMA UNIVERSITY   (Japan)

Author keywords

differentiation; glucagon; glucokinase; glucose transporter 2; pancreatic polypeptide

Indexed keywords

ACTIVIN A; AMYLASE; INSULIN; MESSENGER RNA; PANCREAS POLYPEPTIDE;

AMYLASE RELEASE; ANIMAL CELL; ARTICLE; CELL TYPE; DNA SYNTHESIS; HORMONE DETERMINATION; HUMAN; IMMUNOFLUORESCENCE MICROSCOPY; INSULIN RELEASE; NONHUMAN; PANCREAS CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

EID: 0029864906     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI118591     Document Type: Article

References (37)

1. Le Douarin, N.M. 1988. On the origin of pancreatic endocrine cells. Cell. 53:169-171.
2. Teitelman, G. 1991. Cellular and molecular analysis of pancreatic islet cell lineage and differentiation. Recent Prog. Horm. Res 47:259-297.
3. Polak, J.M., S.R. Bloom, and P J. Marango. 1984. Neuron-specific enolase, a marker for neuroendocrine cells. In Evolution and Tumor Pathology of the Neuroendocrine System. S. Falkmer, R. Hakanson, and F. Sundler, editors. Elsevier Science Publishers, Amsterdam. 51-68.
4. Weidenmann, B., W W. Franke, C. Kuhn, R. Moll, and V.E. Gould. 1986. Synaptophysin: a marker protein for neuroendocrine cell and neoplasms. Proc. Natl. Acad. Sci. USA. 83:3500-3504.
5. Teitelman, G., and J.K. Lee. 1987 Cell lineage analysis of pancreatic islet cell development: glucagon and insulin cells arise from catecholaminergic precursors present in the pancreatic duct. Dev. Biol. 121:454-466.
6. Ashcroft, F.M., and P. Rorsman. 1991. Electrophysiology of the pancreatic β-cell. Prog. Biophys. Mol Biol 54:87-143.
7. Teitelman, G. 1990. Insulin cells of pancreas extend neurites but do not arise from the neuroectoderm. Dev. Biol. 142:368-379.
8. Pictet, R.L., W.R. Clark, R H.Williams, and W.J. Rutter 1972 An ultrastructural analysis of the developing embryonic pancreas. Dev. Biol 29:436-467.
9. Rail, L B., R.L. Pictet, R S. Williams, and W.J. Rutter. 1973. Early differentiation of glucagon-producing cells in embryonic pancreas. Proc. Natl. Acad. Sci. USA. 70:3478-3482.
10. Alpert, S., D. Hanahan, and G. Teitelman. 1988. Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons. Cell 53:295-308.
11. Gittes, G.K , and W.J. Rutter. 1992 Onset of cell-specific gene expression in the developing mouse pancreas. Proc Natl Acad. Sci. USA. 89:1128-1132.
12. Herrera, P.L., J. Huarte, F Sanvito, P. Meda, L. Orci, and J.D Vassalli 1991. Embryogenesis of the murine endocrine pancreas: early expression of pancreatic polypeptide gene. Development 113:1257-1265.
13. Teitelman, G, S. Alpert, J.M. Polak, A. Martinez, and D. Hanahan. 1993. Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neural proteins tyrosine hydroxylase and neuropeptide Y but not pancreatic polypeptide. Development. 118.1031-1039.
14. Upchurch, B.H., G.W Aponte, and A.B. Leiter. 1994. Expression of peptide YY in all four islet cell types in the developing mouse pancreas suggests a common peptide YY-producing progenitor. Development. 120:245-252.
15. Herrera, P.L., J. Huarte, R. Zufferey, A. Nichols, B. Mermillod, J. Philippe, P. Muniesa, F. Sanvito, L. Orci, and J.D. Vassalli. 1994. Ablation of islet endocrine cells by targeted expression of hormone- promoter-driven toxigenes. Proc. Natl. Acad. Sci. USA 91:12999-13003
16. Christophe, J. 1994. Pancreatic tumoral cell line AR42J: an amphicrine model. Am. J. Physiol 266:G963-G971.
17. Logsdon, C.D., J. Mocssncr, J.A. Williams, and I D. Goldfine. 1985. Glucocorticoid increase amylase mRNA level, secretory organelles and secretion in pancreatic acinar AR42J cells. J. Cell Biol. 100:1200-1208.
18. Vale, W., J. Rivier, J. Vaugham, R. McClintock, A. Corrigan, W. Woo, D. Karr, and J. Spiess. 1986. Purification and characterization of FSH-releasing protein from porcine ovarian follicular fluid Nature (Lond.) 321:776-779.
19. Ohnishi, H., N Ohgushi, S. Tanaka, H. Mogami, R. Nobusawa, H. Mashima, M Furukawa, T. Mine, O. Shimada, H. Ishikawa, and I. Kojima 1995. Conversion of amylase-containing rat pancreatic AR42J cells to neuronlike cells by activin A. J. Clin. Invest. 95:2304-2314.
20. Murata, N , K Onomachi, Y. Eto, H. Shibai, and M. Muramatsu. 1988. Expression of erythroid differentiation factor in Chinese hamster ovary cells. Biochem. Biophys Res. Commun. 151:230-235.
21. 20a. Seno, M., H. Tada, M. Kosaka, R. Sasada, K. Igarashi, Y. Shing, J. Folkman, M. Ueda, and H. Yamada. 1996, Human betacellulin, a member of the EGF family dominantly expressed in pancreas and small intestine, is fully active in a monomeric form. Growth Factors. In press.
22. Miyazaki, J., K. Araki, E. Yamato, H. lkegami, T. Asano, Y. Shibasaki, Y. Oka, and K. Yamamura. 1990. Establishment of a pancreatic β-cell line that retains glucose-inducible insulin secretion Endocrinology. 127:126-132
23. Iwashima, Y., A.K. Abiko, S. Seino, J. Takeda, M. Eto, K.S. Polonsky, and I. Makino. 1994. Reduced levels of messenger RNA for calcium channel, glucose transporter-2 and glucokinase are associated with alterations in insulin secretion in fasted rats. Endocrinology 135:1010-1017.
24. 2+ indicators with greatly improved fluorescence properties. J. Biol Chem. 260:3440-3450.
25. McNiel, P.L., M P. McKenna, and D.L. Taylor. 1985. A transient rise in cytosolic calcium follows stimulation of quiescent cells with growth factors J. Cell Biol. 101:372-379.
26. Kanzaki, M., H. Mogami, H. Shibata, and I. Kojima. 1995. Expression of calcium-permeable cation channel CD20 accelerates progression through G, phase in Balb/c 3T3 cells. J. Biol Chem. 270 13099-13100.
27. Yasuda, H., S. Tanaka, H. Onishi, H. Mashima, N. Ogushi, and I. Kojima. 1994. Activin A. a negative regulator of amylase secretion and cell proliferation in rat pancreatic acinar AR42J cells. Am. J Physiol. 267:G220-G226
28. Bradford, M.M 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding Anal. Biochem. 72:248-254.
29. Lovgren, T., I. Hemmia, K. Pettersson, and P. Halonen. 1985. Time-resolved fluorometry in immunoassay In Alternative Immunoassays. W.P. Collins, editor. John Wiley & Sons Ltd., New York. 203-217.
30. Shing, Y., G. Christofori, D. Hanahan, Y. Ono, R. Sasada, K. Igarashi, and J. Folkman. 1993. Betacellulin: a mitogen from pancreatic beta cell tumors. Science (Wash. DC). 259:1604-1607.
31. Wang, T.C., S. Bonner-Weir, P.S. Oates, M.B. Chulak, B. Simon, G T. Merlino, E.V. Schmidt, and S.J. Brand 1993. Pancreatic gastrin stimulates islet differentiation of transforming growth factor-α-induced ductular precursor cells. J Clin. Invest. 92:1349-1356.
32. Watanabe, T., A. Shintani, M. Nakata, Y. Shieng, J. Folkman, K Igarashi, and R. Sasada. 1994. Recombinant human betacellulin. J. Biol. Chem. 269:9966-9973
33. Gu, D., M.-S. Lee, T. Krahl, and N. Sarvetnick. 1994. Transitional cells in the regenerating pancreas. Development. 120:1873-1881.
34. Yasuda. H , K. Inoue, H. Shibata, Y. Takeuchi, Y. Eto, Y. Hasegawa, N. Sekine, Y. Totsuka, T. Mine, E. Ogata, and I. Kojima. 1993. Existence of activin A in A- and D-cells of rat pancreatic islet Endocrinology. 133:624-630.
35. Ogawa, K., K. Ono, M. Kuromatsu, and Y. Hayashi. 1995. Effect of streptozotocin injection on expression of immunoreactive follistatin and βA and βB subunits of inhibin/activin in rat pancreatic islets. Eur. J. Endocrinol. 132:363-369.
36. Furukawa, M., Y. Eto, and I. Kojima. 1995. Expression of activin A in fetal rat pancreas. Endocrine J. 42:63-68
37. Ritvos, O., T. Tuuri, M. Eramaa, K. Sainio, K, Hilen, L. Saxen, and S F Gilbert. 1995. Activin A disrupts epithelial branching morphogenesis in developing glandular organs of the mouse. Mech. Devel. 50:229-245.