Mucosal immunogenicity of genetically detoxified derivatives of heat labile toxin from Escherichia coli

Vaccine

Volumn 16, Issue 11-12, 1998, Pages 1065-1073

  Douce, Gill ^a   Giuliani, Marzia Monica ^b   Giannelli, Valentina ^b   Pizza, Maria Grazia ^b   Rappuoli, Rino ^b   Dougan, Gordon ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b CHIRON VACCINES   (Italy)

Author keywords

Detoxified derivatives; LT K63; Mucosal immunisation

Indexed keywords

ESCHERICHIA ENTEROTOXIN; HEAT LABILE TOXIN; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN SUBCLASS; NEUTRALIZING ANTIBODY; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; TOXOID; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; IMMUNE RESPONSE; IMMUNIZATION; IMMUNOBLOTTING; IMMUNOGENICITY; IMMUNOGLOBULIN PRODUCTION; INTRANASAL DRUG ADMINISTRATION; LUNG LAVAGE; MOUSE; NONHUMAN; ORAL DRUG ADMINISTRATION; PRIORITY JOURNAL; SUBCUTANEOUS DRUG ADMINISTRATION;

EID: 0031802314     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0264-410X(98)80100-X     Document Type: Article

References (25)

1. Spangler, B.D. Structure and function of cholera toxin and related Escherichia coli heat-labile enterotoxin. Microbiol Rev. 1992, 56, 622-647.
2. Spicer, E.K., Kavanaugh, W.M., Dallas, W.S., Falkow, S., Konigsberg, W.H. and Schafer, D.E. Sequence homolgies between A subunits of Escherichia coli and Vibrio cholerae enterotoxins. Proc. Natl. Acad. Sci. USA 1981, 78, 50-54.
3. Sixma, T.K., Pronk, S.E., Kalk, K.H., Wartna, E.S., Van Zanten, B.A., Witholt, B. and Hol, W.G. Crystal structure of cholera related heat labile toxin from E. coli. Nature 1991, 351, 371-377.
4. Sixma, T.K., Kalk, K.H., Vanzanten, B.A.M., Dauter, Z., Kingma, J., Witholt, B. and Hol, W.G.J. Refined structure of Escherchia coli heat-labile enterotoxin, a close relative of cholera toxin. J. Mol. Biol. 1993, 230, 890-918.
5. Levine, M.M., Kaper, J.B., Black, R.E. and Clements, M.L. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiol. Rev. 1983, 47, 510-550.
6. Elson, C.J. and Ealding, W. Generalised systemic and mucosal immunity in mice after mucosal stimulation with cholera toxin. J. Immunol. 1984, 132, 2736-2743.
7. Clements, J.D., Hartzog, N.M. and Lyon, F.L. Adjuvant activity of Escherichia coli heat-labile enterotoxin and effect on the induction of oral tolerance in mice to unrelated protein antigens. Vaccine 1988, 6, 269-277.
8. Lycke, N. and Holmgren, J. Strong adjuvant properties of cholera toxin on gut mucosal immune responses to orally presented antigens. Immunol. 1986, 59, 301-308.
9. Debard, N., Buzoni Gatel, D. and Bout, D. Intranasal immunization with SAG1 protein of Toxoplasma gondii in association with cholera toxin dramatically reduces development of cerebral cysts after oral infection. Infect. Immun. 1996, 64, 2158-2166.
10. Holmes, R.K. and Twiddy, E.M. Characterisation of monoclonal antibodies that react with unique and cross-reacting determinants of cholera toxin and its subunits. Infect. Immun. 1983, 42, 914-923.
11. Peterson, A., Hetjmancik, K.E., Markel, D.E., Craig, J.P. and Kurosky, A. Antigenic specificity of neutralizing antibody to cholera toxin. Infect. Immun. 1979, 24, 774-779.
12. Pierce, N.F., Cray, W.C. and Sacci, J.B. Oral immunisation of dogs with purified cholera toxin, crude cholera toxin, or B-subunit: evidence for synergistic protection by anti-toxic and antibacterial mechanisms. Infect. Immun. 1982, 37, 687-694.
13. Holmgren, J., Svennerholm, A.-M., Jertborn, M., Clemens, J., Sack, D.A., Salenstedt, R. and Wigzel, H. An oral B subunit: whole cell vaccine against cholera. Vaccine 1992, 10, 911-914.
14. Holmgren, J., Svennerholm, A.-M. Sack, D. and Clemens, J. New and Improved Vaccines Against Cholera: The Oral B Subunit/inactivated Whole Cell Vaccine. (Eds Woodrow, G.G. and Levine, M.M.). Marcel Dekker Inc., New York, 1990.
15. Clemens, J., Sack, D.A., Harris, J.R., Chakraborty, J., Neogy, P.K., Stanton, B., Huda, N., Khan, M.U., Kay, B.A., Khan, M.R., Ansaruzzaman, M., Yunus, M., Raghava Rao, M., Svennerholm, A.-M. and Holmgren, J. Cross-protection by B-subunit-whole cell cholera vaccine against diarrhea associated with heat-labile toxin-producing enterotoxigenic Escherichia coli: results of a large scale field trial. J. Infect. Dis. 1988, 158, 372-377.
16. Pizza, M., Fontana, M.R., Giuliani, M.M., Domenighini, M., Magagnoli, C., Giannelli, V., Nucci, D., Hol, W., Manetti, R. and Rappuoli, R. A genetically detoxified derivative of heat-labile Escherichia coli enterotoxin induces neutralizing antibodies against the A subunit. J. Exp. Med. 1994, 180, 2147-2153.
17. Fontana, M.R., Manetti, R., Giannelli, V., Magagnoli, C., Marchini, A., Olivieri, R., Domenighini, M., Rappuoli, R. and Pizza, M. Construction of nontoxic derivatives of cholera toxin and characterization of the immunological response against the A subunit. Infect. Immun. 1995, 63, 2356-2360.
18. De Haan, L., Verweij, W.R., Feil, I.K., Lijnema, T.H., Hol, W.G., Agsteribbe, E. and Wilschut, J. Mutants of the Escherichia coli heat-labile enterotoxin with reduced ADP-ribosylation activity or no activity retain the immunogenic properties of the native holotoxin. Infect. Immun. 1996, 64, 5413-5416.
19. Douce, G., Turcotte, C., Cropley, I., Roberts, M., Pizza, M., Domenghini, M., Rappuoli, R. and Dougan, G. Mutants of Escherichia coli heat-labile toxin lacking ADP-ribosyltransferase activity act as nontoxic, mucosal adjuvants. Proc. Natl. Acad. Sci. USA 1995, 92, 1644-1648.
20. Di Tommaso, A., Saletti, G., Pizza, M., Rappuoli, R., Dougan, G., Abrignani, S., Douce, G. and De Magistris, M.T. Induction of antigen-specific antibodies in vaginal secretions by using a nontoxic mutant of heat-labile enterotoxin as a mucosal adjuvant. Infect. Immun. 1996, 64, 974-979.
21. Giannelli, V., Fontana, M.-R., Giuliani, M.M., Guangcal, D., Rappuoli, R. and Pizza, M. Protease susceptibility and toxicity of heat labile enterotoxins with a mutation in the active site or in the protease sensitive loop. Infect. Immun. 1997, 65, 331-334.
22. Czerskinsky, C., Nilsson, L.-A., Nygren, H., Ouchterlony, O. and Tarkowski, A. A solid phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody secreting cells. J. Immunol. 1983, 65, 109-121.
23. Magagnoli, C., Manetti, R., Fontana, M.R., Giannelli, V., Giuliani, M.M., Rappuoli, R. and Pizza, M. Mutations in the A subunit affect yield, stability, and protease sensitivity of nontoxic derivatives of heat-labile enterotoxin. Infect. Immun. 1996, 64, 5434-5438.
24. Nakagawa, I., Takahashi, I., Kiyono, H., McGhee, J.R. and Hamada, S. Oral immunization with the B subunit of the heat-labile enterotoxin of Escherichia coli induces early Th1 and late Th2 cytokine expression in Peyer's patches. J. Infect. Dis. 1996, 173, 1428-1436.
25. Takahashi, I., Marinaro, M., Kiyono, H., Jackson, R.J., Nakagawa, I., Fujihashi, K., Hamada, S., Clements, J.D., Bost, K.L. and McGhee, J.R. Mechanisms for mucosal immunogenicity and adjuvancy of Escherichia coli labile enterotoxin. J. Infect. Dis. 1996, 173, 627-635.