Purification and genetic characterization of enterocin I from Enterococcus faecium 6T1a, a novel antilisterial plasmid-encoded bacteriocin which does not belong to the pediocin family of bacteriocins

Applied and Environmental Microbiology

Volumn 64, Issue 12, 1998, Pages 4883-4890

  Floriano, Belén ^a   Ruiz Barba, José L ^a   Jiménez Díaz, Rufino ^a  

^a INSTITUTO DE LA GRASA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOCIN; ENTEROCIN 1; MESSENGER RNA; PEDIOCIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; ENTEROCOCCUS FAECIUM; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN FAMILY; PROTEIN PURIFICATION;

BACTERIA (MICROORGANISMS); CLOSTRIDIA; ENTEROCOCCUS FAECIUM; LISTERIA MONOCYTOGENES; POSIBACTERIA; PROKARYOTA;

EID: 0031767745     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.12.4883-4890.1998     Document Type: Article

References (53)

1. Anba, J., E. Bidnenko, A. Hillier, D. Ehrlich, and M. C. Chopin. 1995. Characterization of the lactococcal abiD1 gene coding for phage abortive infection. J. Bacteriol. 177:3818-3823.
2. Anderson, D. G., and L. L. McKay. 1983. Simple and rapid method for isolating large-plasmid DNA from lactic streptococci. Appl. Environ. Microbiol. 46:548-552.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1992. Current protocols in molecular biology. Greene Publishing and Wiley-Interscience, New York, N. Y.
4. Aymerich, T., H. Holo, L. S. Håvarstein, M. Hugas, M. Garriga, and I. F. Nes. 1996. Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins. Appl. Environ. Microbiol. 62:1676-1682.
5. Barefoot, S. F., and T. R. Klaenhammer. 1984. Detection and activity of lactacin B, a bactericin produced by Lactobacillus acidophilus. Antimicrob. Agents Chemother. 26:328-334.
6. Bhunia, A. K., M. C. Johnson, and B. Ray. 1987. Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulphate-polyacrylamide gel electrophoresis. J. Ind. Microbiol. 2:319-322.
7. Booth, M. C., C. P. Bogie, H.-G. Sahl, R. J. Siezen, K. L. Hatter, and M. S. Gilmore. 1996. Structural analysis and proteolytic activation of Enterococcus faecalis cytolysin, a novel lantibiotic. Mol. Microbiol. 21:1175-1184.
8. Casaus, P., T. Nilsen, L. M. Cintas, I. F. Nes, P. E. Hernández, and H. Holo. 1997. Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A. Microbiology 143:2287-2294.
9. Cathcart, D. P. 1995. Purification, characterization and molecular analysis of plantaricin S, a two peptide bacteriocin from olive fermenting Lactobacillus plantarum strains. Ph.D. thesis. Cranfield University, Cranfield, Bedford, United Kingdom.
10. Cintas, L. M., P. Casaus, L. V. Håvarstein, P. E. Hernández, and I. F. Nes. 1997. Biochemical and genetic characterization of enterocin P, a novel secdependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Appl. Environ. Microbiol. 63:4321-4330.
11. 10a. Cintas, L. M., P. Casaus, H. Holo, P. E. Hernandez, I. F. Nes, and L. S. Havarstein. 1998. Enterocins L50A and L50B, two novel bacteriocins from Enterococcus faecium L50, are related to staphylococcal hemolysins. J. Bacteriol. 180:1988-1994.
12. Davidson, B. E., N. Kordias, M. Dobos, and A. J. Hillier. 1996. Genomic organization of lactic acid bacteria. Antoine Leeuwenhoek 70:161-183.
13. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
14. de Vuyst, L., and E. J. Vandamme. 1994. Bacteriocins of lactic acid bacteria. Blackie Academie & Professional, London, England.
15. Geis, A., J. Singh, and M. Teuber. 1993. Potential of lactic streptococci to produce bacteriocin. Appl. Environ. Microbiol. 45:205-211.
16. Gerdes, K., and S. Molin. 1986. Partitioning of plasmid R1. Structural and functional analysis of the parA locus. J. Mol. Biol. 190:269-279.
17. Gierasch, L. M. 1989. Signal sequences. Biochemistry 28:923-930.
18. Hastings, J. W., M. Sailer, K. Johnson, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1991. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostocgelidum. J. Bacteriol. 173:7491-7500.
19. Håverstein, L. S., D. B. Diep, and I. F. Nes. 1995. A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export. Mol. Microbiol. 16:229-240.
20. Hechard, Y., D. B. Derijard, F. Letellier, and Y. Cenatiempo. 1992. Characterization and purification of mesentericin Y105, an anti-Listeria bacteriocin from Leuconostoc mesenteroides. J. Gen. Microbiol. 138:2725-2731.
21. Henderson, J. T., A. L. Chopko, and P. D. van Wasserman. 1992. Characterization and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC1.0. Arch. Biochem. Biophys. 295:5-12.
22. Holck, A., L. Axelsson, S. E. Birkeland, T. Aukrust, and H. Blom. 1992. Purification and amino acid sequence of sakacin A, a bacteriocin from Lactobacillus sake Lb 706. J. Gen. Microbiol. 138:2715-2720.
23. Hoover, G. D., and L. R. Steenson. 1993. Bacteriocins of lactic acid bacteria. Academic Press, Inc., San Diego, Calif.
24. Izard, J. W., and D. A. Kendall. 1994. Signal peptides: exquisitely designed transport promoters. Mol. Microbiol. 13:765-773.
25. Jack R. W., J. R. Tagg, and B. Ray. 1995. Bacteriocins of gram-positive bacteria. Microbiol. Rev. 59:171-200.
26. Jiménez-Díaz, R., R. M. Rios-Sánchez, M. Desmazeau, J. L. Ruiz-Barba, and J. C. Piard. 1993. Plantaricins S and T, two new bacteriocins produced by Lactobacillus plantarum LPCO10 isolated from a green olive fermentation. Appl. Environ. Microbiol. 59:1416-1424.
27. Jiménez-Díaz, R., J. L. Ruiz-Barba, D. P. Cathcart, H. Holo, I. F. Nes, K. H. Sletten, and P. J. Warner. 1995. Purification and partial amino acid sequence of plantaricin S, a bacteriocin produced by Lactobacillus plantarum LPCO10, the activity of which depends on the complementary action of two peptides. Appl. Environ. Microbiol. 61:4459-4463.
28. Joeger, M., and T. R. Klaenhammer. 1986. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J. Bacteriol. 167:439-446.
29. Joosten, H. M. L., M. Núñez, B. Devreese, J. van Beeumen, and J. D. Marugg. 1996. Purification and characterization of enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4. Appl. Environ. Microbiol. 62: 4220-4223.
30. Kersters, K., and J. de Ley. 1975. Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. J. Gen. Microbiol. 87:333-342.
31. Klaenhammer, T. R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12:39-86.
32. Lipman, D. J., and W. R. Pearson. 1985. Rapid and sensitive protein similarity searches. Science 227:1435-1441.
33. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
34. Martínez-Bueno, M., M. Maqueda, A. Gálvez, B. Samyn, J. Van Beeumen, J. Coyette, and E. Valdivia. 1994. Determination of the gene sequence and the molecular structure of the enterococcal peptide antibiotic AS-48. J. Bacteriol. 176:6334-6339.
35. Merril, C. R., D. Goldman, S. A. Sedman, and M. H. Ebert. 1981. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437-1438.
36. Mortvedt, C. I., J. Nissen-Meyer, K. Sletten, and I. F. Nes. 1991. Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45. Appl. Environ. Microbiol. 57:1829-1834.
37. Nes, I. F., D. B. Diep, L. S. Håvarstein, M. B. Brurberg, V. Eijsink, and H. Holo. 1996. Biosynthesis of bacteriocins in lactic acid bacteria. Antonie Leeuwenhoek 70:113-128.
38. Piard, J.-C., F. Delorme, G. Giraffa, J. Commisaire, and M. Desmazeaud. 1990. Evidence for a bacteriocin produced by Lactococcus lactis CNRZ 481. Neth. Milk Diary J. 44:143-158.
39. Piard, J.-C., P. M. Muriana, M. J. Desmazeaud, and T. R. Klaenhammer. 1992. Purification and partial characterization of lactacin 481, a lanthionine-containing bacteriocin produced by Lactococcus lactis subsp. lactis CNRZ 481. Appl. Environ. Microbiol. 58:279-284.
40. Pot, B., C. Hertel, W. Ludwig, P. Descheemaeker, K. Kersters, and K. H. Schleifer. 1993. Identification and classification of Lactobacillus acidophilus, L. gasseri and L. johnsonii strains by SDS-PAGE and rRNA-targeted oligo-nucleotide probe hybridization. J. Gen. Microbiol. 139:513-517.
41. Pot, B., P. Vandamme, and K. Kersters. 1994. Analysis of electrophoretic whole-organism protein fingerprints, p. 493-521. In M. Goodfellow and A. G. O'Donnel (ed.), Chemical methods in prokaryotic systematics. John Wiley & Sons, Inc., Chichester, England.
42. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
43. Quadri, L. E. N., M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1994. Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV17B. J. Biol. Chem. 269:12204-12211.
44. Rauch, P. J. G., M. M. Beerthuyzen, and W. M. De Vos. 1990. Nucleotide sequence of IS904 Lactococcus lactis subsp. lactis strain NIZO RS. Nucleic Acids Res. 18:4253-4254.
45. Ruiz-Barba, J. L., J. C. Piard, and R. Jiménez-Díaz. 1991. Plasmid profile and curing of plasmids in Lactobacillus plantarum strains isolated from green olive fermentations. J. Appl. Bacteriol. 71:417-421.
46. Samyn, B., M. Martínez-Bueno, B. Devreese, M. Maqueda, A. Gálvez, E. Valdivia, J. Coyette, and J. van Beeumen. 1994. The cyclic structure of the enterococcal peptide antibiotic AS-48. FEBS Lett. 352:87-90.
47. Schägger, H., and G. Von Jagow. 1987. Tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
48. Tagg, J. R., A. S. Dajani, and L. W. Wannamaker. 1976. Bacteriocins of gram-positive bacteria. Bacteriol. Rev. 40:722-756.
49. Tichaczek, P. S., R. F. Vogel, and W. P. Hammes. 1994. Cloning and sequencing of sakP encoding sakacin P, the bacteriocin produced by Lactobacillus sake LTH 673. Microbiology 140:361-367.
50. Tomita, H., S. Fujimoto, K. Tanimoto, and V. Ike. 1996. Cloning and genetic organization of the bacteriocin 31 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pYI17. J. Bacteriol. 178: 3585-3593.
51. van Belkum, M. J., R. W. Worobo, and M. E. Stiles. 1997. Double-glycine-type leader peptides direct secretion of bacteriocins by ABC transporters: colicin V secretion in Lactococcus lactis. Mol. Microbiol. 23: 1293-1301.
52. von Heijne, G. 1986. Net N-C charge imbalance may be important for signal sequence function in bacteria. J. Mol. Biol. 192:287-290.
53. Worobo, R. W., T. Henkel, M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. 1994. Characteristics and genetic determinant of a hydrophobic peptide bacteriocin, carnobacteriocin A, produced by Carnobacterium piscicola LV17A. Microbiology 140:517-526.